Rat anti-Mouse LAG3 Monoclonal Antibody | anti-LAG3 antibody

LAG3 (Lymphocyte Activation Gene 3 Protein, LAG-3, Protein FDC, CD223, FDC)

Cat. Num. AAA642763

• Gene Names: LAG3; CD223
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Synonyms: LAG3; Monoclonal Antibody; LAG3 (Lymphocyte Activation Gene 3 Protein; LAG-3; Protein FDC; CD223; FDC); Anti -LAG3 (Lymphocyte Activation Gene 3 Protein; anti-LAG3 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 12K124
• Specificity: Recognizes mouse LAG-3. Does not react with recombinant human LAG-3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Form/Format: Supplied as a lyophilized powder from PBS pH7.4, 5% trehalose. Reconstitute with 200ul sterile PBS
• Concentration: ~0.5mg/ml after reconstitution (varies by lot)

• Applicable Applications for anti-LAG3 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 2.5ug labels10e6 cells
• Immunogen: Recombinant preotein corresponding to Ser23-Leu442 from mouse LAG-3 expressed in NS0 cell line.
• Accession No: Q61790
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Flow Cytometry (FC/FACS)

(Detection of LAG-3 in mouse pplenocytes by Flow Cytometry. Mouse splenocytes were treated for 72h with 50ng/ml PMA and 500ng/ml Ca2+ Ionomycin then stained with MBS642763 or isotype control antibody, followed by Allophycocyanin- conjugated Rat IgG Secondary Antibody.)

Related Product Information for anti-LAG3 antibody

LAG-3 is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496aa membrane protein with a 421aa extracellular region which contains four IgSF domains, a 21aa transmembrane region and a 54aa cytoplasmic region. LAG-3 shares <20% aa sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.

Product Categories/Family for anti-LAG3 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 167614500
• NCBI GeneID: 3902
• NCBI Accession #: NP_002277.4
• NCBI GenBank Nucleotide #: NM_002286.5
• UniProt Accession #: P18627; A8K7T9
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte-activation gene 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• Protein Family: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3
• UniProt Entry Name: LAG3_HUMAN

NCBI Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; antigen binding; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation