Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12 A431 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of F12 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Desmosome/Hemidesmosome Recombinant Antibody
Anti-Desmosome/Hemidesmosome [F12]
Reactivity
Human
Applications
Immunofluorescence
Purity
Affinity Purified, Using A Recombinant Lectin Column
Synonyms
Desmosome/Hemidesmosome; Monoclonal Recombinant Antibody; Anti-Desmosome/Hemidesmosome [F12]; Desmosome/Hemidesmosome recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Human IgM kappa
Clone Number
F12
Specificity
F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a MW of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate), and by IEM (immunoelectron microscopy) it was determined that it is the intracellular part of the desmosome that is bound (Gilbert, 1997). F12 shares some immunochemical properties with autoantibodies present in paraneoplastic pemphigus sera. Pemphigus diseases are those resulting in blistering of the skin due to the production of autoantibodies against keratinocyte surface proteins - autoantibodies of patients with pemphius vulgaris recognize desmoglein 1 (Dsg1), a major glycoprotein of the desmosome complex.
Purity/Purification
Affinity Purified, Using A Recombinant Lectin Column
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for Desmosome/Hemidesmosome recombinant antibody
Immunofluorescence (IF), Immunoelectron Microscopy (IEM)
Antibody Type
Recombinant Antibody
Antibody Species
Human
Original Species Name
Human
Immunogen
F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.
Clone Note
F12 labels the keratinocyte membrane and the basement membrane zone of rat tongue sections by indirect immunofluorescence (IIF). The antibody also stains both the cell membrane and the basement membrane zone of stratified squamous epithelia, and other epithelial tissues such as urinary bladder, small bowel, thymus, and liver. Non-epithelial tissues, such as myocardium are additionally stained. Sera from patients with pemphigus vulgaris can be incubated with human skin sections to determine their capacity to block the binding of F12 in an IIF blocking assay. IEM (immunoelectron microscopy) can be used to determine the ultralocalization of the antibody.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12 A431 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of F12 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12 A431 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of F12 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for Desmosome/Hemidesmosome recombinant antibody
Recombinant monoclonal antibody to desmosome/hemidesmosome. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma F12.
Product Categories/Family for Desmosome/Hemidesmosome recombinant antibody
References
Gilbert et al. Production of a Human Monoclonal Anti-epithelial Cell Surface Antibody Derived from a Patient with Pemphigus Vulgaris Journal of Autoimmunity (1992) 5,173-182 PMID:1627232
Original Published Journal: Journal of Autoimmunity (1992) 5,173-182
Original Published Journal: Journal of Autoimmunity (1992) 5,173-182
Original Published PMID: 9174592
Publication Note: Describes the generation of an anti-desmosome IgM antibody which recognises a 185 kDa protein which was also recognized by the sera of two patients with pemphigus vulgaris. Its binding properties were studied.
Original Published Journal: Journal of Autoimmunity (1992) 5,173-182
Original Published Journal: Journal of Autoimmunity (1992) 5,173-182
Original Published PMID: 9174592
Publication Note: Describes the generation of an anti-desmosome IgM antibody which recognises a 185 kDa protein which was also recognized by the sera of two patients with pemphigus vulgaris. Its binding properties were studied.
Similar Products
Product Notes
The Desmosome/Hemidesmosome (Catalog #AAA488464) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Desmosome/Hemidesmosome [F12] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Desmosome/Hemidesmosome can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunoelectron Microscopy (IEM). Researchers should empirically determine the suitability of the Desmosome/Hemidesmosome for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Desmosome/Hemidesmosome, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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