Rat anti-Mouse IL2R gamma C Monoclonal Antibody | anti-IL2RgammaC antibody

Anti-IL2R gamma C [3E12]

Cat. Num. AAA488671

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: IL2R gamma C; Monoclonal Antibody; Anti-IL2R gamma C [3E12]; CD132; common gamma chain; common y chain; gamma chain; IL-2 receptor gamma chain; Interleukin-2 receptor subunit gamma; Cytokine receptor common subunit gamma; Il2rg; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; gammaC; p64; anti-IL2RgammaC antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b, kappa
• Clone Number: 3000000000000
• Specificity: This conformation-dependent antibody binds to the murine common gamma-chain, shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptor complexes. It recognizes an epitope located in a membrane-proximal portion of the common gamma-chain, near Arg-197 of domain 2. This antibody does not cross-react with COS7 cells transfected with rat common gamma-chain cDNA (Olosz et al, 2002).
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IL2RgammaC antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Blocking (BL)
• Application Notes: This antibody has been used in flow cytometric analysis of common gamma-chain surface expression by IL-7-stimulated T cells (Goh et al, 2017) and COS7 monkey kidney cells (Olosz et al, 2002). This antibody has also been used to immunoprecipiate murine common gamma-chain-containing complexes from COS7 cell extracts (Olosz et al, 2002). Additionally, as this antibody is conformationally sensitive, it has been used to confirm the correct folding of soluble murine common gamma chain mutants (Meibetaner et al, 2001). This antagonistic antibody is most effective at inhibiting IL-4 bioactivity (Malek et al, 1998). Treatment of recipient mice with this antibody, along with another common gamma-chain-blocking MAb (4G3), significantly prolongs islet allograft survival (Li et al, 2000). Indeed, blocking the common gamma chain with this antibody has been shown to induce apoptotic cell death of BAF-B03 cells and primary anti-CD3 activated T lymphoblasts in vitro, and of alloreactive T cells in vivo (Li et al, 2000).
• PMID: 7530740
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed mouse splenocytes with anti-IL2R gamma C antibody 3E12 (MBS488670) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3E12 (MBS488670) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488670, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-IL2RgammaC antibody

Recombinant monoclonal antibody to IL2R gamma C. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3E12.
This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing murine cytoplasmic-tailless IL-2R common gamma-chain (YB2/0-myt).

Product Categories/Family for anti-IL2RgammaC antibody

interleukins; cytokine receptor

References

He et al. Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor. J Immunol. 1995 Feb 15;154(4):1596-605.
Note on publication: Describes the original generation of this antibody, and its use in determining murine common gamma-chain lymphoid cell distribution.

NCBI and Uniprot Product Information

• NCBI GI #: 7305181
• NCBI GeneID: 16186
• NCBI Accession #: NP_038591.1
• NCBI GenBank Nucleotide #: NM_013563.3
• UniProt Accession #: P34902
• Molecular Weight: 42,241 Da
• NCBI Official Full Name: cytokine receptor common subunit gamma
• UniProt Protein Name: Cytokine receptor common subunit gamma
• UniProt Gene Name: Il2rg
• UniProt Synonym Gene Names: IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG
• UniProt Entry Name: IL2RG_MOUSE

NCBI Description

This gene encodes a transmembrane protein that is a common subunit of several interleukin receptor complexes. These receptors are comprised of alpha and beta subunits in addition to this gamma subunit. Signalling through this pathway in important in immune cell differentiation and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

IL2RG: Common subunit for the receptors for a variety of interleukins. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell- negative (XSCID); also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell- mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID). XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID. Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-7 binding; interleukin-2 binding

Biological Process: positive regulation of T cell differentiation in the thymus; positive regulation of lymphocyte differentiation; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; regulation of gene expression; positive regulation of B cell differentiation