Rat anti-Mouse CD80 Monoclonal Antibody | anti-CD80 antibody

Anti-CD80 [RM80]

Cat. Num. AAA488603

• Gene Names: Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, Functional Assay
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: CD80; Monoclonal Antibody; Anti-CD80 [RM80]; B7-1; Activation B7-1 antigen; B7; B7 protein; B7-1 protein; B7.1; Cd28l; CD28LG; CD28LG1; LAB7; Ly-53; Ly53; MIC17; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; anti-CD80 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a, kappa
• Clone Number: RM80
• Specificity: This antibody is specific for murine CD80, a ~55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to the activation of T and B cells, respectively.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD80 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC), Blocking (BL), Functional Assay (FA)
• Application Notes: This antibody has been used in multiple FACS experiments for various immunological applications, such as to highlight the robustness of the T cell priming process by activated Nlrc5-deficient dendritic cells (Rota et al, 2016), to demonstrate that allogeneic IgG combined with dendritic cell stimuli could induce anti-tumor T cell immunity (Carmi et al, 2015), to confirm the role of aquaporin-9-expressing neutrophils for the development of contact hypersensitivity (Moniaga et al, 2015), and to support the therapeutic potential of targeting B-cell Syk signaling in chronic graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015), as well as in in vitro blocking experiments to confirm the role of CD28 signals for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) gammadelta T cell subsets (Ribot et al, 2012). Furthermore, this antibody has been used, together with the anti-CD86 antibody, in numerous in vivo studies, such as in a NZB/W F1 mouse lupus model to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al, 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al, 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al, 2005).
• PMID: 7489744
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD80 antibody RM80 (B467) (MBS488602) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RM80 (MBS488602) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488602, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-CD80 antibody

Recombinant monoclonal antibody to CD80. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RM80.
This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.

Product Categories/Family for anti-CD80 antibody

immunology; costimulation; immune-checkpoint; T cells; B cells

References

Atsuo Nakajima et al. Preferential dependence of autoantibody production in murine lupus on CD86 costimulatory molecule. Eur J Immunol. 1995 Nov;25(11):3060-9.
Note on publication: Describe the use of this antibody, together with the anti-CD86 antibody, to confirm the preferential dependence of murine Ab-mediated autoimmunity on the CD86 costimulatory molecule.

NCBI and Uniprot Product Information

• NCBI GI #: 112983684
• NCBI GeneID: 12519
• NCBI Accession #: NP_033985.3
• NCBI GenBank Nucleotide #: NM_009855.2
• UniProt Accession #: Q00609; Q61332
• Molecular Weight: 24,051 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 antigen
• NCBI Official Symbol: Cd80
• NCBI Official Synonym Symbols: B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: Cd80
• UniProt Synonym Gene Names: B7; B7

Uniprot Description

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16 B4|16 26.86 cM

Cellular Component: cell surface; external side of plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of alpha-beta T cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell proliferation; T cell costimulation