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Type
Reactivity
Gene Name
Isotype
Host
Application
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4087 results for "Inflammation" - showing 150-200
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-OX40L antibody R4930 (Oxelumab) (Ab00536) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R4930 (Ab00536-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
OX40L, Recombinant Antibody (Cat# AAA488213)
Full Name
Anti-OX40L [R4930 (Oxelumab)]
Gene Names
TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data #1
(Human peripheral blood lymphocytes stained with Mouse anti Human siglec-10 (MBS224495) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
SIGLEC-10, Monoclonal Antibody (Cat# AAA224376)
Full Name
MOUSE ANTI HUMAN SIGLEC-10:FITC
Gene Names
SIGLEC10; SLG2; PRO940; SIGLEC-10
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
SDS-Page
(SDS-PAGE Analysis Purified TIM3 Mouse Monoclonal Antibody (TIM3/2399). Confirmation of Purity and Integrity of Antibody.)
TIM3/HAVCR2/CD366, Monoclonal Antibody (Cat# AAA4381360)
Full Name
TIM3/HAVCR2/CD366 (Effector T-Cell Marker)
Gene Names
HAVCR2; TIM3; CD366; KIM-3; SPTCL; TIMD3; Tim-3; TIMD-3; HAVcr-2
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215793)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA211045)
Full Name
RAT ANTI HUMAN CD18:FITC
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$415/100 Tests | $1,710/5x100 Tests
Procalcitonin (PCT), Monoclonal Antibody (Cat# AAA7111074)
Full Name
Mouse Anti-Human Procalcitonin (PCT) Monoclonal Antibody
Applications
EIA, CLIA
Purity
>95%, Protein G purified
Pricing
$230/1 mg | $985/5x1 mg
Activity Data
(Macrophage migration inhibitory factor (MIF), also known as glycosylation- inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG- coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MIF with MHCDG.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105111)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Skin stained with Tryptase Mouse Monoclonal Antibody (TPSAB1/1963).)
Tryptase, Monoclonal Antibody (Cat# AAA4381312)
Full Name
Tryptase (Mast Cell Marker)
Gene Names
TPSAB1; TPS1; TPS2; TPSB1; TPSB2; Tryptase-2
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CD97 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CD97, Polyclonal Antibody (Cat# AAA129021)
Full Name
CD97 Polyclonal Antibody
Gene Names
CD97; TM7LN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD11a research biosimilar antibody Efalizumab (hu1124, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD11a, Recombinant Antibody (Cat# AAA488211)
Full Name
Anti-CD11a [hu1124 (Efalizumab)]
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Anti-CD97 antibody IHC of human Thyroid, Follicular Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
CD97, Polyclonal Antibody (Cat# AAA243436)
Full Name
Rabbit Polyclonal to Human CD97
Gene Names
CD97; TM7LN1
Reactivity
Gibbon, Dog, Gorilla, Human, Monkey
Predicted Reactivity: Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Predicted Reactivity: Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Applications
IHC - Paraffin, ICC
Purity
Immunoaffinity Purified
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215799)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216593)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
CD200R, Recombinant Antibody (Cat# AAA488073)
Full Name
Anti-CD200R [OX108]
Gene Names
CD200R1; OX2R; MOX2R; CD200R; HCRTR2
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD62L (L-selectin) antibody MEL-14 Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
CD62L, Monoclonal Recombinant Antibody (Cat# AAA488435)
Full Name
Anti-CD62L [MEL-14]
Gene Names
Sell; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
Reactivity
Mouse
Applications
BL, IP, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215790)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of AGER Antibody (N-term) in mouse lung tissue lysates (35ug/lane). AGER (arrow) was detected using the purified Pab.)
AGER, Polyclonal Antibody (Cat# AAA9210446)
Full Name
AGER Antibody (N-term)
Gene Names
AGER; RAGE
Reactivity
Human, Mouse
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
CD97, Polyclonal Antibody (Cat# AAA244923)
Full Name
Rabbit Polyclonal to Human CD97
Gene Names
CD97; TM7LN1
Reactivity
Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Applications
IHC - Paraffin, ICC
Purity
Immunoaffinity Purified
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Lymphotoxin alpha, Monoclonal Recombinant Antibody (Cat# AAA488356)
Full Name
Anti-Lymphotoxin alpha [3F12.2D3]
Gene Names
LTA; LT; TNFB; TNFSF1; TNLG1E
Reactivity
Human
Applications
EIA, FC/FACS, SPR
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
( Immunohistochemical staining of rat spleen tissue using anti-CD11a antibody hu1124 (Efalizumab). Anti-CD11a staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of hu1124 was used to stain samples at a concentration of 3 ug/ml.)
CD11a, Recombinant Antibody (Cat# AAA488210)
Full Name
Anti-CD11a [hu1124 (Efalizumab)]
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215796)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215794)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$280/0.05 mg | $1,115/5x0.05 mg
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216590)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
FC/FACS, IF, WB
Pricing
$525/0.25 mg | $2,210/5x0.25 mg
Testing Data
(Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 (MBS215395A647). following permeablisation with Leucoperm)
CD68, Monoclonal Antibody (Cat# AAA215404)
Full Name
RAT ANTI MOUSE CD68:RPE
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-alpha-4 integrin antibody 21.6 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
alpha-4 Integrin, Monoclonal Recombinant Antibody (Cat# AAA488448)
Full Name
Anti-alpha-4 Integrin [21.6]
Gene Names
ITGA4; IA4; CD49D
Reactivity
Human
Applications
EIA, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-alpha-4 integrin antibody 21.6 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 (MBS488449) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488449, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
alpha-4 Integrin, Monoclonal Recombinant Antibody (Cat# AAA488449)
Full Name
Anti-alpha-4 Integrin [21.6]
Gene Names
ITGA4; IA4; CD49D
Reactivity
Human
Applications
EIA, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Serum amyloid A Protein (SAA1), Monoclonal Antibody (Cat# AAA7111073)
Full Name
Mouse Anti-Human Serum amyloid A Protein (SAA1) Monoclonal Antibody
Applications
EIA, CLIA, LETIA
Purity
>95%, Protein G purified
Pricing
$215/1 mg | $925/5x1 mg
RANKL, Monoclonal Recombinant Antibody (Cat# AAA488483)
Full Name
Anti-RANKL [IK22-5]
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Reactivity
Mouse
Applications
IHC, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 (MBS215395A647). following permeablisation with Leucoperm)
CD68, Monoclonal Antibody (Cat# AAA215406)
Full Name
RAT ANTI MOUSE CD68:RPE
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216594)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
FC/FACS
Pricing
$980/500 Tests | $4,250/5x500 Tests
RANKL, Monoclonal Recombinant Antibody (Cat# AAA488484)
Full Name
Anti-RANKL [IK22-5]
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Reactivity
Mouse
Applications
IHC, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD62L (L-selectin) antibody MEL-14 Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (MBS488436, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
CD62L, Monoclonal Recombinant Antibody (Cat# AAA488436)
Full Name
Anti-CD62L [MEL-14]
Gene Names
Sell; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
Reactivity
Mouse
Applications
BL, IP, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescent staining of PFA-fixed HeLa cells with Endoglin / CD105 Mouse Monoclonal Antibody (ENG/1621) followed by goat anti-mouse IgG-CF488 (Green). )
Endoglin/CD105, Monoclonal Antibody (Cat# AAA4381689)
Full Name
Endoglin/CD105 (Angiogenesis Marker)
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Human, Monkey, Horse, Pig, Rat
Applications
EIA, FC/FACS, IF
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
CD162, Monoclonal Antibody (Cat# AAA4381244)
Full Name
CD162 (Selectin P Ligand)
Gene Names
SELPLG; CLA; CD162; PSGL1; PSGL-1
Reactivity
Human
Applications
FC/FACS, IF
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Immunohistochemistry (IHC)-Formallin
(Formalin-fixed, paraffin-embedded human tonsil stained with OX40 Mouse Monoclonal Antibody (OX40/3427).)
OX40/CD134/TNFRSF4, Monoclonal Antibody (Cat# AAA4381684)
Full Name
OX40/CD134/TNFRSF4 (Immuno-Oncology Target)
Gene Names
TNFRSF4; OX40; ACT35; CD134; IMD16; TXGP1L
Reactivity
Human, Chimpanzee
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)-Formallin
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Mouse Monoclonal Antibody (rECM1/792).)
Secretory Component/ECM1, Monoclonal Antibody (Cat# AAA4381537)
Full Name
Secretory Component/ECM1
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)-Formallin
(Formalin-fixed, paraffin-embedded human liver stained with ECM1 Recombinant Mouse Monoclonal Antibody (rSPM217). )
Secretory Component/ECM1, Monoclonal Antibody (Cat# AAA4381630)
Full Name
Secretory Component/ECM1
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
SDS-Page
(SDS-PAGE Analysis Purified CD162 Recombinant Mouse Monoclonal Antibody (rPSGL1/1601). Confirmation of Purity and Integrity of Antibody.)
CD162, Monoclonal Antibody (Cat# AAA4381560)
Full Name
CD162 (Selectin P Ligand)
Gene Names
SELPLG; CLA; CD162; PSGL1; PSGL-1
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using OX40 Mouse Monoclonal Antibody (OX40/3428). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. )
OX40/CD134/TNFRSF4, Monoclonal Antibody (Cat# AAA4381614)
Full Name
OX40/CD134/TNFRSF4 (Immuno-Oncology Target)
Gene Names
TNFRSF4; OX40; ACT35; CD134; IMD16; TXGP1L
Reactivity
Human, Chimpanzee
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
IL-1 BETA, Polyclonal Antibody (Cat# AAA220072)
Full Name
RABBIT ANTI CHICKEN INTERLEUKIN-1 BETA:Preservative Free
Gene Names
IL1B; IL1beta; IL-1BETA
Applications
EIA, FN, WB
Pricing
$610/0.5 mg | $2,585/5x0.5 mg
alpha 1 Acid Glycoprotein, Native Protein (Cat# AAA537846)
Full Name
alpha 1 Acid Glycoprotein protein
Gene Names
Orm1; AGP; OMD; Orm; Agpa1
Applications
User optimized
Purity
> 95% pure
Pricing
$480/10 mg | $2,110/5x10 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with a-1-Antitrypsin (AAT) antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of endothelial cells.)
alpha 1 Antitrypsin, Polyclonal Antibody (Cat# AAA534535)
Full Name
alpha 1 Antitrypsin antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Human
Applications
IHC
Pricing
$1,140/1 mL | $5,120/5x1 mL
Testing Data #1
(Western blot analysis of WNV transfected (B) and untransfected (A) Hela cell lysates probed with Rabbit anti WNV core protein (MBS223747))
WNV, Polyclonal Antibody (Cat# AAA223747)
Full Name
RABBIT ANTI WNV CORE PROTEIN (C-TERMINAL)
Applications
EIA, IF, WB
Pricing
$430/0.1 mg | $1,780/5x0.1 mg
Hepatitis B Virus preS1, Monoclonal Antibody (Cat# AAA532473)
Full Name
Hepatitis B Virus preS1 antibody
Reactivity
Specific for HBV preS1 , (subtype adw)
Applications
EIA
Pricing
$395/1 mg | $1,760/5x1 mg
HBsAg, Native Protein (Cat# AAA537021)
Full Name
HBsAg protein (Subtype ad)
Applications
User optimized
Purity
> 95% by SDS-PAGE
Pricing
$405/1 mg | $1,805/5x1 mg
Testing Data
(Release of monocytes from bone marrow during infection is driven by MCP1 (CCL2).)
MCP1, Monoclonal Antibody (Cat# AAA530038)
Full Name
MCP1 antibody
Reactivity
Human MCP1
Applications
EIA, WB
Pricing
$645/0.5 mg | $2,895/5x0.5 mg
Testing Data
(Peripheral human granulocytes stained with Mouse anti Human CD97:APC)
CD97, Monoclonal Antibody (Cat# AAA215311)
Full Name
MOUSE ANTI HUMAN CD97:APC
Gene Names
CD97; TM7LN1
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Published customer image: Assessment of dectin-2 function on inflammatory cells. A) Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/M˜ (Ly-6G-). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments. B) Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜ from dectin-1-deficient mice. All bars show mean+/-95% confidence intervals of dectin-2-blocked cells relative to isotype control treated cells (100%, dotted line) from 3 independent experiments (raw representative data, which includes the isotype control data, from one of the 3 independent experiments are shown in Figure S2). Data were analysed by two-way ANOVA, two assess the differences caused by complement opsonization (˜C') or the use of different cell lineages (˜L'). Samples in which the 95% confidence intervals do not overlap with the mean isotype control are specific indicated with a # symbol.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-2, Monoclonal Antibody (Cat# AAA211248)
Full Name
RAT ANTI MOUSE DECTIN-2:Low Endotoxin
Gene Names
Clec4n; Nkcl; Clec6a; Clecsf10
Applications
FC/FACS, IP
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
OPG, Polyclonal Antibody (Cat# AAA535773)
Full Name
OPG antibody
Gene Names
TNFRSF11B; OPG; TR1; OCIF; PDB5
Reactivity
human OPG
Applications
EIA, WB
Pricing
$425/0.05 mg | $1,905/5x0.05 mg
Rabies, Recombinant Protein (Cat# AAA538386)
Full Name
Rabies protein
Applications
EIA, WB
Purity
> 95% by SDS-PAGE
Pricing
$290/0.1 mg | $1,290/5x0.1 mg
