RANKL recombinant antibody

Anti-RANKL [IK22-5]

Cat. Num. AAA488483

• Gene Names: Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
• Reactivity: Mouse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Neutralization Assay
• Purity: Protein A Affinity Purified
• Synonyms: RANKL; Monoclonal Recombinant Antibody; Anti-RANKL [IK22-5]; CD254; IK22/5; RANK Ligand; Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; TNF-related activation-induced cytokine; TRANCE; RANKL recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: IK22-5
• Specificity: This antibody is specific for murine RANKL.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 316

• Applicable Applications for RANKL recombinant antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Neutralising
• Antibody Type: Recombinant Antibody
• Antibody Species: Rabbit
• Original Species Name: Rat
• Immunogen: Thus antibody was raised by immunising an SD rat with murine RANKL-transfected baby hamster kidney cells.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Clone Note: This antibody has been used in immunohistochemical analysis of frozen Peyer's patch tissue sections (Kobayashi et al, 2013). It has also been used in flow cytometric analysis of RANKL cell surface expression by Th1 and Th17 cells (Nakae et al, 2007), RANKL-transfected human embryonic kidney cells (Miyahira et al, 2003) and murine T hybridoma 2B4.11 cells (Fionda et al, 2007). Additionally, this RANKL-neutralising antibody has been used to assess the effects of RANKL inhibition on myoepithelial cell proliferation in mammary organoid culture (Haslam et al, 2008). Treatment of collagen-induced arthritis (CIA) mice with this antibody has been shown to reduce bone loss at the site of inflammation (Kamijo et al, 2006), while administration to C57BL/6J mice induced transient tibia and skull bone growth arrest and irreversible blockage of tooth eruption (Lezot et al, 2015).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Related Product Information for RANKL recombinant antibody

Recombinant monoclonal antibody to RANKL. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma IK22-5.

Product Categories/Family for RANKL recombinant antibody

Autoimmunity; Bone Growth

References

Kamijo et al. Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody. Biochem Biophys Res Commun. 2006 Aug 18;347(1):124-32. PMID:16815304
Original Published Journal: Biochem Biophys Res Commun. 2006 Aug 18;347(1):124-32.
Publication Note: Describes the original generation of this antibody, and the characterisation of its effects when administered to collagen-induced arthritis mice.

NCBI and Uniprot Product Information

• NCBI GI #: 114842415
• NCBI GeneID: 21943
• NCBI Accession #: NP_035743.2
• NCBI GenBank Nucleotide #: NP_035743.2
• UniProt Accession #: O35235
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: ODF; OPGL; RANKL; Ly109l; Trance
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_MOUSE

Uniprot Description

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; integral to membrane; plasma membrane; intracellular

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoclast differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of JNK cascade; mammary gland epithelial cell proliferation; osteoclast differentiation; lymph node development; activation of JNK activity; positive regulation of corticotropin-releasing hormone secretion; activation of NF-kappaB transcription factor; positive regulation of homotypic cell-cell adhesion; calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; monocyte chemotaxis; positive regulation of MAP kinase activity; organ morphogenesis; regulation of osteoclast differentiation; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; positive regulation of T cell activation; cell differentiation; positive regulation of phosphorylation; protein homooligomerization; bone resorption