Testing Data #1
(Western blot analysis of WNV transfected (B) and untransfected (A) Hela cell lysates probed with Rabbit anti WNV core protein (MBS223747))
Rabbit WNV Polyclonal Antibody | anti-WNV antibody
RABBIT ANTI WNV CORE PROTEIN (C-TERMINAL)
Applications
ELISA, Immunofluorescence, Western Blot
Synonyms
WNV; Polyclonal Antibody; RABBIT ANTI WNV CORE PROTEIN (C-TERMINAL); anti-WNV antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises the 14kDa core protein of West Nile Virus (WNV). WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis.
West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway.
West Nile Virus is spread by mosquitos and is believed to have originated in the West Nile region of Africa but has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.
West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway.
West Nile Virus is spread by mosquitos and is believed to have originated in the West Nile region of Africa but has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
3430
Applicable Applications for anti-WNV antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Western Blot: This item detects a band of approximately 14kDa in Transfected HeLa cell lysate
ELISA: Maximum Dilution: 1ug/ml
Immunofluorescence: Maximum Dilution: 20ug/ml
Western Blotting: Maximum Dilution: 1ug/ml
ELISA: Maximum Dilution: 1ug/ml
Immunofluorescence: Maximum Dilution: 20ug/ml
Western Blotting: Maximum Dilution: 1ug/ml
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to an 15 amino acid sequence from near the carboxy terminus of West Nile Virus core protein.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antiserum to human West Nile Virus core protein was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Viral
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Western blot analysis of WNV transfected (B) and untransfected (A) Hela cell lysates probed with Rabbit anti WNV core protein (MBS223747))
Testing Data #1
(Western blot analysis of WNV transfected (B) and untransfected (A) Hela cell lysates probed with Rabbit anti WNV core protein (MBS223747))
Testing Data #2
(Immunofluorescent staining of WNV transfected Vero cells with Rabbit anti WNV core protein (MBS223747))
Testing Data #2
(Immunofluorescent staining of WNV transfected Vero cells with Rabbit anti WNV core protein (MBS223747))
Related Product Information for anti-WNV antibody
MBS223747 recognises the 14kDa core protein of West Nile Virus (WNV). WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis. West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway. West Nile Virus is spread by mosquitos and is believed to have originated in the West Nile region of Africa but has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
380,110 Da
NCBI Official Full Name
unnamed protein product
NCBI Official Symbol
WNVgp1
NCBI Protein Information
polyprotein precursor; capsid protein C; anchored capsid protein C; membrane glycoprotein precursor prM; membrane glycoprotein M; envelope protein E; nonstructural protein NS1; nonstructural protein NS2A; nonstructural protein NS2B; nonstructural protein NS3; nonstructural protein NS4A; 2K protein; nonstructural protein NS4B; nonstructural protein NS5
UniProt Protein Name
Genome polyprotein
UniProt Gene Name
NS1
UniProt Synonym Gene Names
NS2A; NS4A; NS4B
UniProt Entry Name
POLG_WNV
Uniprot Description
Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA.
Research Articles on WNV
Similar Products
Product Notes
The WNV ns1 (Catalog #AAA223747) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's WNV can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Western Blot: This item detects a band of approximately 14kDa in Transfected HeLa cell lysate ELISA: Maximum Dilution: 1ug/ml Immunofluorescence: Maximum Dilution: 20ug/ml Western Blotting: Maximum Dilution: 1ug/ml. Researchers should empirically determine the suitability of the WNV ns1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
