Rabbit CD97 Polyclonal Antibody | anti-CD97 antibody

Rabbit Polyclonal to Human CD97

Cat. Num. AAA244923

• Gene Names: CD97; TM7LN1
• Reactivity: Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Immunocytochemistry
• Purity: Immunoaffinity Purified
• Synonyms: CD97; Polyclonal Antibody; Rabbit Polyclonal to Human CD97; Anti-CD97 Antibody (N-Terminus) IHC-plus; CD97 molecule; Leukocyte antigen CD97; TM7LN1; CD97 antigen; Human CD97; anti-CD97 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CD97. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAP3K12 (58%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 835

• Applicable Applications for anti-CD97 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)
• Application Notes: ICC, IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 19 amino acid peptide from N-terminal extracellular domain of human CD97. Percent identity with other species by BLAST analysis: Human (100%); Monkey (95%).
• Immunogen Type: Synthetic peptide
• Immunogen: CD97 antibody was raised against synthetic 19 amino acid peptide from N-terminal extracellular domain of human CD97. Percent identity with other species by BLAST analysis: Human (100%); Monkey (95%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CD97 antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Transfection

(Anti-CD97 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with CD97.)

Transfection Control

(Anti-CD97 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Related Product Information for anti-CD97 antibody

CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform.

Product Categories/Family for anti-CD97 antibody

Family: GPCR; Subfamily: CD55

NCBI and Uniprot Product Information

• NCBI GI #: 17978491
• NCBI GeneID: 976
• NCBI Accession #: NP_510966.1
• NCBI GenBank Nucleotide #: NM_078481.3
• UniProt Accession #: P48960; O00718; O76101; Q8NG72; Q8TBQ7; A8K7Z4; B2RBJ9
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: CD97 antigen isoform 1 preproprotein
• NCBI Official Synonym Full Names: CD97 molecule
• NCBI Official Symbol: CD97
• NCBI Official Synonym Symbols: TM7LN1
• NCBI Protein Information: CD97 antigen; leukocyte antigen CD97; seven-span transmembrane protein; seven transmembrane helix receptor; seven-transmembrane, heterodimeric receptor associated with inflammation
• UniProt Protein Name: CD97 antigen
• Protein Family: CD97 antigen
• UniProt Gene Name: CD97
• UniProt Entry Name: CD97_HUMAN

NCBI Description

This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]

Uniprot Description

CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: extracellular space; focal adhesion; membrane; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; cell-cell signaling; immune response; cell motility; inflammatory response; cell adhesion