Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
2050 results for "Controls" - showing 150-200
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Adrenal Gland stained with StAR Mouse Monoclonal Antibody (STAR/2140).)
StAR (Steroidogenic Acute Regulator), Monoclonal Antibody (Cat# AAA4380494)
Full Name
StAR (Steroidogenic Acute Regulator) (Leydig Cell Marker)
Gene Names
STAR; STARD1
Reactivity
Human. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE )
CD8 BETA CHAIN, Monoclonal Antibody (Cat# AAA224819)
Full Name
MOUSE ANTI PIG CD8 BETA CHAIN: FITC
Gene Names
CD8B; LY3; P37; LEU2; LYT3; CD8B1
Reactivity
Pig
Applications
FC
Pricing
$560/0.1 mg | $2,365/5x0.1 mg
Testing Data
(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: APC )
CD4, Monoclonal Antibody (Cat# AAA214751)
Full Name
RAT ANTI DOG CD4:RPE
Applications
FC
Pricing
$695/100 Tests | $2,975/5x100 Tests
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488 (MBS212208A488))
CD105, Monoclonal Antibody (Cat# AAA215082)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
FC/FACS
Pricing
$195/0.025 mg | $730/5x0.025 mg
Hepatitis Virus (MHV), Positive Control (Cat# AAA412554)
Full Name
Mouse Hepatitis Virus (MHV) PCR Control
Pricing
$1,260/0.25 mL | $5,470/5x0.25 mL
Testing Data
(25 ug of HeLa cell lysate spiked with 3.5 ug of mouse IgG was run in lanes 2, 3, 5 and 6 under reducing conditions on SDS PAGE using the V3 system and transferred to PVDF membrane. Precision Plus MW marker was run in lanes 1 and 4. Mouse anti actin gamma (VMA00049) was used to detect actin gamma (approximate MW of 46 kDa) in lanes 2 and 5 only. Lanes 3 and 6 were used as controls to detect the background signaling of the secondary detection agent (no primary antibody control).. TidyBlot Western Blot Detection Reagent:HRP was used as the secondary reagent in lanes 2 and 3 and an HRP conjugated anti-mouse IgG heavy and light chain antibody was used as the secondary in lanes 5 and 6. The Blot was visualized with the Chemidoc. The TidyBlot Western Blot Detection Reagent:HRP only bound to the native actin gamma antibody whereas the standard anti-Mouse IgG heavy and light recognized both the native and denatured antibodies)
TidyBlot WESTERN BLOT DETECTION REAGENT, Accessory Reagent (Cat# AAA224768)
Full Name
TidyBlot WESTERN BLOT DETECTION REAGENT: HRP
Applications
WB
Pricing
$170/0.05 mL | $220/0.5 mL | $325/1 mL | $845/5x0.5 mL | $1,305/5x1 mL | $625/5x0.05 mL
Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
CD335, Monoclonal Antibody (Cat# AAA211240)
Full Name
MOUSE ANTI BOVINE CD335:Low Endotoxin
Gene Names
NCR1; NKP46
Applications
FC/FACS, FN, IF, WB
Pricing
$755/0.5 mg | $3,255/5x0.5 mg
Western Blot (WB)
(Western blot detection of Tubulin beta in Hela, 3T3, C6, CHO-K1 and COS7 cell lysates using Tubulin beta mouse mAb conjugated with HRP (1:10000 diluted), Predicted band size:55KDa.Observed band size:55KDa.)
beta-Tubulin, Monoclonal Antibody (Cat# AAA475342)
Full Name
Anti-beta-Tubulin-HRP (HRP-Conjugate) Mouse mAb
Gene Names
TUBB; M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Reactivity
Human, Mouse, Monkey, Hamster, Rat
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Adrenal Gland stained with StAR Mouse Monoclonal Antibody (STAR/2154).)
StAR (Steroidogenic Acute Regulator), Monoclonal Antibody (Cat# AAA4380495)
Full Name
StAR (Steroidogenic Acute Regulator) (Leydig Cell Marker)
Gene Names
STAR; STARD1
Reactivity
Human. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF)
(MBS9600576 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
APC, Polyclonal Antibody (Cat# AAA9600576)
Full Name
Phospho-APC(Ser2054) Antibody
Gene Names
APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
Reactivity
Human
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of mouse liver tissue lysate using Rabbit anti MMP-2 antibody at a 1/10000 dilution. 3% non-fat dry milk in TBST was used for blocking)
MMP-2, Polyclonal Antibody (Cat# AAA225599)
Full Name
Rabbit Anti MMP-2
Gene Names
MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Reactivity
Human
Applications
IHC, WB
Purity
Purified IgG was prepared from whole serum by affinity chromatography.
Pricing
$430/0.05 mL | $1,780/5x0.05 mL
Western Blot (WB)
(Anti-SCNN1A Antibody (Center) at 1:2000 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
SCNN1A, Polyclonal Antibody (Cat# AAA9214278)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RB:FITC (MBS210617))
CD45RB, Monoclonal Antibody (Cat# AAA212028)
Full Name
MOUSE ANTI HUMAN CD45RB
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Reacts with: Rhesus Monkey, Monkey; Does not react with: Horse, Goat, Llama
N.B. Antibody reactivity and working conditions may vary between species.
N.B. Antibody reactivity and working conditions may vary between species.
Applications
FC/FACS, IP
Purity
Purified IgG prepared by affinity chromatography on Protein A.
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
Testing Data
(Immunofluorescence staining of a human tonsil cryostetion with Mouse anti Human CD40 antibody, clone LOB7/6 (MBS212239), red in A and Mouse anti Human CD21 antibody, clone LB21 (MBS212051), green in B. The merged image is C with nuclei counterstained blue using DAPI. Medium power)
CD40, Monoclonal Antibody (Cat# AAA215112)
Full Name
MOUSE ANTI HUMAN CD40:RPE
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
FC/FACS
Pricing
$240/25 Tests | $930/5x25 Tests
Immunohistochemistry (IHC)
(MBS9609341 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MYST3, Polyclonal Antibody (Cat# AAA9609341)
Full Name
MYST3 Antibody
Gene Names
KAT6A; MOZ; MRD32; MYST3; MYST-3; ZNF220; RUNXBP2; ZC2HC6A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
hapten 4-hydroxy-3-nitrophenyl acetyl (NP), Recombinant Antibody (Cat# AAA488057)
Full Name
Anti-hapten 4-hydroxy-3-nitrophenyl acetyl (NP) [B1-8]
Applications
WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
CD335, Monoclonal Antibody (Cat# AAA213799)
Full Name
MOUSE ANTI BOVINE CD335:RPE
Gene Names
NCR1; NKP46
Applications
FC/FACS
Pricing
$640/100 Tests | $2,735/5x100 Tests
Activity Data
(Figure. The binding activity of TXLNa with PDIA3.Taxilin Alpha (TXLNa) also known as interleukin-14 (IL-14) is a cytokine that controls the growth and proliferation of both normal and cancerous B cells. TXLNa induces B-cell proliferation, inhibits antibody secretion, and expands selected B-cell subgroups. This interleukin is produced mainly by T cells and certain malignant B cells. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of TXLNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TXLNa and recombinant human PDIA3. Briefly, TXLNa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TXLNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TXLNa and PDIA3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Taxilin Alpha (TXLNa), Active Protein (Cat# AAA2105130)
Full Name
Active Taxilin Alpha (TXLNa)
Gene Names
TXLNA; IL14; TXLN
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$225/0.01 mg | $480/0.05 mg | $740/0.1 mg | $915/0.2 mg | $1,790/0.5 mg | $2,645/1 mg | $6,465/5 mg
Testing Data
(Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC (MBS219809))
CD63, Monoclonal Antibody (Cat# AAA219672)
Full Name
MOUSE ANTI RAT CD63
Applications
FC/FACS, IP, WB
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Western Blot (WB)
ATPB, Monoclonal Antibody (Cat# AAA435190)
Full Name
ATPB Mouse mAb
Gene Names
ATP5F1B; ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human, Cotton, Honeybee, Peach, Hedge bamboo, Azumapecten farreri, Wheat, Rice, Tomato, Zebrafish, Silk moth, Tobacoo, Mouse-ear cressn
Applications
WB, IF, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD299: Azide Free (MBS210211))
CD229, Monoclonal Antibody (Cat# AAA212512)
Full Name
MOUSE ANTI HUMAN CD229
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Applications
FC/FACS, IP
Pricing
$525/0.2 mg | $2,210/5x0.2 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213058)
Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213845)
Full Name
RAT ANTI HUMAN CD18:RPE
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)
(Western blot analysis of lysate from EPHB2 protein, using EphB2 Antibody (Cat. #MBS9200496). AM7623b was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35?g.)
EPHB2, Monoclonal Antibody (Cat# AAA9200496)
Full Name
EPHB2 Antibody
Gene Names
EPHB2; DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5
Reactivity
Human
Applications
FC/FACS, EIA, IHC-P, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216587)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
FC/FACS
Pricing
$280/0.05 mg | $1,115/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3beta mouse mAb (1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)
GSK3 beta, Monoclonal Antibody (Cat# AAA475481)
Full Name
Anti-GSK3 beta Mouse mAb
Reactivity
Human, Mouse
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
ATP synthase subunit gamma, Monoclonal Antibody (Cat# AAA435251)
Full Name
ATP synthase subunit gamma Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216585)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Low Endotoxin
Applications
FC/FACS, IF, WB
Pricing
$830/0.5 mg | $3,580/5x0.5 mg
Immunohistochemistry (IHC)
(Sample Type: human optic nerves and spinal cordA/[IHC-PZ] (optimal processing)human optic nerve (short post mortem interval) fixed by immersion in zinc-based fixative (BD Pharmingen 552658), processed to minimize antigen loss (shortened protocol, reduced exposure to high temperature); embedded in paraffin wax; sectioned at 3 micronsA2/[IHC-PZ + FF]As above for initial fixation then post-fixed in 10% buffered formalin for 5 daysB/ [IHC-P]human optic nerve and spinal cord fixed in 10% buffered formalin (relatively short post mortem interval and fixation duration); standard processing; embedded in paraffin wax; sectioned at 3 micronsControlsNegative: omission of primary.Positive: Olig2 reactivity was in comparison with antibody from Prof Charles Stiles/ Dr John Alberta (DF308; N terminal Olig2) used at 1:10000 (IHC-PZ); 1:4000 (IHC-P))
SOX10, Polyclonal Antibody (Cat# AAA3201449)
Full Name
SOX10 Antibody - middle region
Gene Names
SOX10; DOM; WS4; PCWH; WS2E; WS4C
Reactivity
Reactivity: Human
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells using 14-3-3 theta antibody.)
14-3-3 theta, Polyclonal Antibody (Cat# AAA9604951)
Full Name
14-3-3 theta Antibody
Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (TD)
CD15, Monoclonal Antibody (Cat# AAA370019)
Full Name
CD15
Reactivity
Paraffin, Frozen
Pricing
$335/7 mL (RTU) | $225/0.1 mL (Concentrate) | $395/0.5 mL (Concentrate) | $585/1 mL (Concentrate) | $515/15 mL (RTU) | $245/3 mL (RTU) | $2,630/5x1 mL (Concentrate)
Western Blot (WB)
(Figure 1. Western blot analysis of Cyclophilin B using anti-Cyclophilin B antibody (MBS1750821).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human U-87MG whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: mouse HEPA1-6 whole cell lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse NIH3T3 whole cell lysates,Lane 8: mouse SP20 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin B antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin B at approximately 19KD. The expected band size for Cyclophilin B is at 24KD. )
Cyclophilin B, Polyclonal Antibody (Cat# AAA1750821)
Full Name
Anti-Cyclophilin B Picoband Antibody
Gene Names
PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(MBS9600408 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
APC, Polyclonal Antibody (Cat# AAA9600408)
Full Name
APC Antibody
Gene Names
APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD16: Biotin (MBS210312))
CD16, Monoclonal Antibody (Cat# AAA212047)
Full Name
MOUSE ANTI HUMAN CD16
Gene Names
FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
Applications
FC/FACS, IP
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
SDS-Page
(SDS-PAGE Analysis Purified ACE/CD143 Mouse Monoclonal Antibody (9B9). Confirmation of Integrity and Purity of Antibody.)
Angiotensin I Converting Enzyme (ACE)/CD143, Monoclonal Antibody (Cat# AAA4380833)
Full Name
Angiotensin I Converting Enzyme (ACE)/CD143
Gene Names
ACE; DCP; ACE1; DCP1; CD143
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Very Low Density Lipoprotein, Protein (Cat# AAA173148)
Full Name
Very Low Density Lipoprotein (VLDL), Human Plasma
Gene Names
VLDLR; CARMQ1; CHRMQ1; VLDLRCH
Pricing
$225/10 mg | $405/100 mg | $1,395/1 g | $6,095/5x1 g
Testing Data
(Published customer image: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERbeta1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERbeta1, ERbeta2 and ERbeta5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.)
ESTROGEN RECEPTOR BETA 2, Monoclonal Antibody (Cat# AAA219425)
Full Name
MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 2
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Applications
Immunohistology Paraffin*
Pricing
$265/0.05 mg | $1,035/5x0.05 mg
Prealbumin, Protein (Cat# AAA173178)
Full Name
Prealbumin/Transthyretin, Human Serum
Gene Names
LCN1; TP; TLC; PMFA; VEGP
Purity
>= 70% (SDS-PAGE)
Pricing
$165/0.1 mg | $205/1 mg | $660/10 mg | $3,865/100 mg | $17,220/5x100 mg
Testing Data
(Immunofluorescence staining of a human tonsil cryostetion with Mouse anti Human CD40 antibody, clone LOB7/6 (MBS212239), red in A and Mouse anti Human CD21 antibody, clone LB21 (MBS212051), green in B. The merged image is C with nuclei counterstained blue using DAPI. Medium power)
CD40, Monoclonal Antibody (Cat# AAA210742)
Full Name
MOUSE ANTI HUMAN CD40:FITC
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data #1
(Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (P-Selectin) (MBS224591))
CD62P, Monoclonal Antibody (Cat# AAA224591)
Full Name
MOUSE ANTI HUMAN CD62P
Gene Names
SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
Applications
FC/FACS, IF, WB
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of LOVO, using GSK3B antibody. The lane on the left is treated with the antigen-specific peptide.)
GSK3B, Polyclonal Antibody (Cat# AAA9607870)
Full Name
GSK3B Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(TTBK2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TTBK2 antibody detected the TTBK2 protein (arrow).)
TTBK2, Polyclonal Antibody (Cat# AAA9206888)
Full Name
TTBK2 Antibody (N-term)
Gene Names
TTBK2; TTBK; SCA11
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Staining of human peripheral blood platelets with Mouse anti Human CD42b:FITC (MBS211082))
CD42b, Monoclonal Antibody (Cat# AAA213138)
Full Name
MOUSE ANTI HUMAN CD42b
Gene Names
GP1BA; BSS; GP1B; VWDP; CD42B; GPIbA; BDPLT1; BDPLT3; DBPLT3; CD42b-alpha
Applications
EIA, FC/FACS, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Activity Data
(Figure1. TRAP levels in the cell of RAW264.7 cells induced by RANKL.Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a member of the tumor necrosis factor (TNF) superfamily. RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is an apoptosis regulator gene, a binding partner of osteoprotegerin (OPG), a ligand for the receptor RANK and controls cell proliferation by modifying protein levels of Id4, Id2 and cyclin D1. The protein can bind to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. Thus, we use recombinant RANKL to induce RAW264.7 mouse monocyte/macrophage cells to differentiate to osteoclast cell. RAW264.7 mouse monocyte/macrophage cells were seeded into 24-well plates at a density of 2×105 and allowed to attach overnight, then treated with RANKL (1ng/mL, 10ng/mL) and incubated for 72h. Then we assay TRAP (a kind of enzyme expressed by osteoclast cell) by ELASA. Result: TRAP levels in the cell supernatant of RAW264.7 cells increased significantly after stimulated with RANK, the data was shown in Table 1 and Figure1.)
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), Active Protein (Cat# AAA2105123)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
hapten 4-hydroxy-3-nitrophenyl acetyl (NP), Recombinant Antibody (Cat# AAA488059)
Full Name
Anti-hapten 4-hydroxy-3-nitrophenyl acetyl (NP) [B1-8]
Applications
WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane.Antibodies: 1012 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
CXCR4, Polyclonal Antibody (Cat# AAA151582)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse
Applications
EIA, WB, IF
Purity
CXCR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA219819)
Full Name
RAT ANTI HUMAN CD18:Low Endotoxin
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, FN, IP
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216583)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using RFXANK antibody (Red), compared to a nonspecific negative control antibody (Blue).)
RFXANK, Monoclonal Antibody (Cat# AAA832055)
Full Name
RFXANK antibody
Gene Names
RFXANK; BLS; RFX-B; ANKRA1; F14150_1
Applications
IF, FC/FACS, IHC, WB
Purity
RFXANK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Leptin, Active Protein (Cat# AAA691546)
Full Name
Mouse Leptin
Gene Names
Lep; ob; obese
Reactivity
Mouse
Purity
> 95% (SDS-PAGE; HPLC)
Pricing
$250/0.2 mg | $395/1 mg | $1,565/5x1 mg
