CD4 Monoclonal Antibody | anti-CD4 antibody

RAT ANTI DOG CD4:RPE

Cat. Num. AAA214751

• Applications: Flow Cytometry
• Synonyms: CD4; Monoclonal Antibody; RAT ANTI DOG CD4:RPE; anti-CD4 antibody

• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: YKIX302.9
• Specificity: CD4
• Form/Format: RPE; Purified IgG conjugate ro R. Phycoerythrin (RPE)- lyophilized

• Applicable Applications for anti-CD4 antibody: Flow Cytometry (FC)
• Application Notes: FC: Neat-1/10; Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It isrecommended that the user titrates the antibody for use in their own system using appropriatenegative/positive controls; ; Flow Cytometry||Use 10ul of the suggested working dilution to label 10^6 cells or 100ul whole blood.
• Target Species: Dog
• Reconstitution: Reconstitute with 1.0ml distilled
• Max Ex/Em: Fluorophore: RPE 488nm laser; Excitation Max (nm): 496; Emission Max (nm): 578
• Immunogen: Canine concanavilin A activated T cell blast.
• Preparation: Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
• Buffer Solution: Phosphate buffered saline
• Preservative Stabilizer: 0.09% Sodium Azide; 1% Bovine Serum Albumin; 5% Sucrose
• Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
• Preparation and Storage: Prior to reconstitution store at 4°C. Following reconstitution store at 4°C. Do not freeze. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommended microcentrifugation before use.

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: APC )

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: FITC (MBS214750))

Testing Data

(Published customer image: Survival rates of animals with canine mammary carcinoma. Kaplan-Meier survival curves for animals for All (MC-BMT and MC) animals categorized according to the relative percentage of CD4+ T-cells (33.3%) (A). Animals were submitted to quarterly follow-ups during twelve months and survival rates expressed in days between the surgical excisions of the end of follow-up as described in Methods. The survival curves were estimated with the Kaplan-Meier method followed by Log-rank test. Correlation analysis highlighted the significant association between the percentages of CD4+ T-cells and CD8+ T-cells with the animal survival in days (B).From: Estrela-Lima et al. BMC Cancer 2010 10:256.)

Testing Data

(Published customer image: Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: Estrela-Lima et al. BMC Cancer 2010 10:256.)

Testing Data

(Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: Alexa Fluor 488 )

Related Product Information for anti-CD4 antibody

Rat anti Dog CD4 antibody (YKIX302.9) is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4. Rat anti Dog CD4 (YKIX302.9) has been demonstrated to partially deplete circulating T lymphocytes when administered in vivo, but alone was not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993). Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocytes subsets Moore et al. 1992. Rat anti Canine CD4 (YKIX302.9) forms part of a panel of anti canine monoclonal antibodies used extensively in the evaluation of leukemic status in dogs (Villiers 2002).

NCBI and Uniprot Product Information

• NCBI GI #: 55742794
• NCBI GeneID: 403931
• NCBI Accession #: NP_001003252.1
• NCBI GenBank Nucleotide #: NM_001003252.1
• UniProt Accession #: P33705
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Symbol: CD4
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_CANFA

Uniprot Description

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.