Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE )
Mouse anti-Pig CD8 BETA CHAIN Monoclonal Antibody | anti-CD8 antibody
MOUSE ANTI PIG CD8 BETA CHAIN: FITC
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Where the product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their system using appropriate negative/positive controls.
Excitation (nm): 490
Emission Max (nm): 525
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.
Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE )
Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE )
Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:FITC (This product))
Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:FITC (This product))
Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain followed by Goat anti Mouse IgG (H/L):FITC )
Flow Cytometry (FC)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain followed by Goat anti Mouse IgG (H/L):FITC )
Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine alpha/beta T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.
NCBI and Uniprot Product Information
NCBI Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
Uniprot Description
CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2p12
Cellular Component: early endosome membrane; external side of plasma membrane; extracellular region; integral to plasma membrane; plasma membrane; T cell receptor complex
Molecular Function: coreceptor activity; MHC class I protein binding; protein binding
Biological Process: adaptive immune response; immune response; regulation of defense response to virus by virus; regulation of immune response; T cell activation; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction
Research Articles on CD8
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Product Notes
The CD8 cd8b (Catalog #AAA224819) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI PIG CD8 BETA CHAIN: FITC reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 BETA CHAIN can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). Flow Cytometry: Neat- 1/10 Use 10ul of the suggested working dilution to label 1x106 cells in 100ul Where the product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD8 cd8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 BETA CHAIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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