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3143 results for " Kinase Assays" - showing 150-200
Typical Testing Data/Standard Curve (for reference only)
PRKAalpha1, ELISA Kit (Cat# AAA2502316)
Full Name
Human PRKAalpha1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Myeloperoxidase (MPO), ELISA Kit (Cat# AAA2022673)
Full Name
Mouse Myeloperoxidase (MPO) ELISA Kit
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
Arginase (ARG), ELISA Kit (Cat# AAA2024616)
Full Name
Human Arginase (ARG) ELISA Kit
Reactivity
Human
Activity Data
(Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. Although gelatin zymography is mainly used for the detection of the MMP2 and MMP9, it also can be used for MMP13 dection. Briefly, various concentrations of MMP13 (1000ng, 500ng, 250ng, 125ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze gelatin nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant rat MMP13 was shown in figure 1.)
Matrix Metalloproteinase 13 (MMP13), Active Protein (Cat# AAA2097264)
Full Name
Active Matrix Metalloproteinase 13 (MMP13)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Typical Testing Data/Standard Curve (for reference only)
PKIA, ELISA Kit (Cat# AAA2515374)
Full Name
Rat PKIA (Protein Kinase Inhibitor Alpha) ELISA Kit
Gene Names
PKIA; PRKACN1
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
EFNA1, ELISA Kit (Cat# AAA2506414)
Full Name
Human EFNA1 (Ephrin A1) ELISA Kit
Gene Names
EFNA1; B61; EFL1; ECKLG; EPLG1; LERK1; LERK-1; TNFAIP4
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
creatine kinase, muscle, ELISA Kit (Cat# AAA930166)
Full Name
Human Creatine kinase M-type, CKM ELISA Kit
Gene Names
CKM; CKMM; M-CK
Reactivity
Human
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-EGFR antibody 528 HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 528 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analysed on a FACSCanto flow-cytometer.)
EGFR, Recombinant Antibody (Cat# AAA488090)
Full Name
Anti-EGFR [528]
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
IF, IHC, IP, WB, FC/FACS
Purity
Protein A Affinity Purified
Typical Testing Data/Standard Curve (for reference only)
PDXK, ELISA Kit (Cat# AAA2515526)
Full Name
Human PDXK (Pyridoxal Kinase) ELISA Kit
Gene Names
PDXK; PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124
Reactivity
Human
Activity Data
(ARSF (Arylsulfatase F) is a member of the sulfatase gene family, and more specifically, the arylsulfatase subfamily. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Besides, EP300 (E1A binding protein p300) has been identified as an interactor of ARSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSF and recombinant human EP300. Briefly, ARSF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ARSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSF and EP300 was shown in Figure 1, and this effect was in a dose dependent manner.)
Arylsulfatase F (ARSF), Active Protein (Cat# AAA2097319)
Full Name
Active Arylsulfatase F (ARSF)
Gene Names
ARSF; ASF
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Typical Testing Data/Standard Curve (for reference only)
PKB, ELISA Kit (Cat# AAA2512912)
Full Name
Rat PKB (Protein Kinase B) ELISA Kit
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Rat
Activity Data
(Figure. The binding activity of AHCY with MHCB.Adenosylhomocysteinase (S-adenosylhomocysteine synthase, S-adenosylhomocysteine hydrolase, adenosylhomocysteine hydrolase, S-adenosylhomocysteinase, SAHase, AdoHcyase, AHCY) is an enzyme that converts S-adenosylhomocysteine to homocysteine and adenosine. The enzyme contains one tightly bound NAD per subunit. Besides, Major Histocompatibility Complex Class I B (MHCB) has been identified as an interactor of AHCY, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AHCY and recombinant human MHCB. Briefly, AHCY were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCB-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AHCY pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of AHCY and MHCB was shown in Figure 1, and this effect was in a dose dependent manner.)
Adenosylhomocysteinase (AHCY), Active Protein (Cat# AAA2105187)
Full Name
Active Adenosylhomocysteinase (AHCY)
Gene Names
AHCY; SAHH; adoHcyase
Applications
Cell Culture, Activity Assays
Purity
> 95%
Typical Testing Data/Standard Curve (for reference only)
BMPR2, ELISA Kit (Cat# AAA2505787)
Full Name
Rat BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit
Gene Names
BMPR2; BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
CKM, ELISA Kit (Cat# AAA2508529)
Full Name
Mouse CKM (Creatine Kinase, Muscle) ELISA Kit
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
Tyk-2, ELISA Kit (Cat# AAA2509641)
Full Name
Human Tyk-2 (Tyrosine Kinase 2) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
VEGFR3/Flt4, ELISA Kit (Cat# AAA2509710)
Full Name
Mouse VEGFR3/Flt4 (Vascular Endothelial Cell Growth Factor Receptor 3) ELISA Kit
Gene Names
FLT4; PCL; FLT41; LMPH1A; VEGFR3
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
RIPK2, ELISA Kit (Cat# AAA2510137)
Full Name
Human RIPK2 (Receptor TNFRSF Interacting Serine Threonine Kinase 2) ELISA Kit
Gene Names
RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
Reactivity
Human
Activity Data
(Deoxyribonuclease I (usually called DNase I) is a nonspecific endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. DNase I can be activated by bivalent metals such as Mg2 and Ca2 . This endonuclease enzyme is common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. For example, DNase I is frequently used to remove template DNA following in vitro transcription, and to remove contaminating DNA in total RNA preparations (especially those from transfected cells that may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR. Besides, Actin Beta (ACTb) has been identified as an interactor of DNase I, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DNase I and recombinant human ACTb. Briefly, DNase I were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-DNase I pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of DNase I and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Deoxyribonuclease I (DNASE1), Active Protein (Cat# AAA2097290)
Full Name
Active Deoxyribonuclease I (DNASE1)
Gene Names
DNASE1; DNL1; DRNI
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Typical Testing Data/Standard Curve (for reference only)
CDKN2A, ELISA Kit (Cat# AAA2507495)
Full Name
Rat CDKN2A (Cyclin Dependent Kinase Inhibitor 2A) ELISA Kit
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
NTRK2, ELISA Kit (Cat# AAA2501604)
Full Name
Rat NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit
Gene Names
NTRK2; TRKB; trk-B; GP145-TrkB
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
ERK1, ELISA Kit (Cat# AAA2503277)
Full Name
Rat ERK1(Extracellular Signal Regulated Kinase 1) ELISA Kit
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
Western Blot (WB)
(Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody.)
PKM2, Polyclonal Antibody (Cat# AAA9405683)
Full Name
PKM2(phospho-Ser37) Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human
Applications
WB, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Typical Testing Data/Standard Curve (for reference only)
EGFR, ELISA Kit (Cat# AAA2513791)
Full Name
Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Sequence
Lemur Tyrosine Kinase 3 (LMTK3), Active Protein (Cat# AAA2086088)
Full Name
Active Lemur Tyrosine Kinase 3 (LMTK3)
Gene Names
LMTK3; LMR3; TYKLM3; PPP1R101
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>95%
Typical Testing Data/Standard Curve (for reference only)
Aprotinin (AP), ELISA Kit (Cat# AAA2021478)
Full Name
Bovine Aprotinin (AP) ELISA Kit
Reactivity
Bovine
Typical Testing Data/Standard Curve (for reference only)
PKD2, ELISA Kit (Cat# AAA2508703)
Full Name
Mouse PKD2 (Protein Kinase D2) ELISA Kit
Gene Names
PKD2; PC2; PKD4; Pc-2; APKD2; TRPP2
Reactivity
Mouse
Sequence Information
Tyrosine Protein Kinase 7 (PTK7), Recombinant Protein (Cat# AAA2086828)
Full Name
Recombinant Tyrosine Protein Kinase 7 (PTK7)
Gene Names
PTK7; CCK4; CCK-4
Applications
WB, May be suitable for use in other assays to be determined by the end user.
Purity
>= 90%
Sequence
Glutathione S Transferase Pi (GSTp), Active Protein (Cat# AAA2097250)
Full Name
Active Glutathione S Transferase Pi (GSTp)
Applications
Cell culture; Activity Assays; In vivo assays
Purity
>95%
Activity Data
(Figure. The binding activity of ODC with TK1.Ornithine decarboxylase (ODC) is an enzyme can catalyze the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. The ornithine decarboxylation reaction catalyzed by ornithine decarboxylase is the first and committed step in the synthesis of polyamines, particularly putrescine, spermidine and spermine. Polyamines are important for stabilizing DNA structure, the DNA double strand-break repair pathway and as antioxidants. Therefore, ornithine decarboxylase is an essential enzyme for cell growth, producing the polyamines necessary to stabilize newly synthesized DNA. Lack of ODC causes cell apoptosis in embryonic mice, induced by DNA damage. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ODC and recombinant human TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105137)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
ODC1; ODC
Applications
Cell Culture, Activity Assays
Purity
> 90%
Sequence
Peptidylglycine Alpha Amidating Monooxygenase (PAM), Active Protein (Cat# AAA2105174)
Full Name
Active Peptidylglycine Alpha Amidating Monooxygenase (PAM)
Gene Names
Pam; PHM
Reactivity
Rattus norvegicus (Rat)
Applications
Cell culture; Activity Assays
Purity
>96%
Activity Data
(Figure. The binding activity of CAPN3 with TTN.Calpain 3 is a calcium-dependent cysteine protease mainly expressed in skeletal muscle. In humans, calpain 3 is encoded by the CAPN3 gene. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. Besides, Titin (TTN) has been identified as an interactor of CAPN3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAPN3 and recombinant human TTN. Briefly, CAPN3 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TTN-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CAPN3 and TTN was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 3 (CAPN3), Active Protein (Cat# AAA2105179)
Full Name
Active Calpain 3 (CAPN3)
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Activity Data
(Figure 1. The binding activity of CKB with CKM.Creatine Kinase, Brain (CKB) also known as CK-BB is a creatine kinase. CKB, consists of a homodimer of two identical brain-type CK-B subunits, is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. Besides, Creatine Kinase, Muscle (CKM) has been identified as an interactor of CKB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CKB and recombinant human CKM. Briefly, CKB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CKM-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CKB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CKB and CKM was shown in Figure 1, and this effect was in a dose dependent manner.)
Creatine Kinase, Brain (CKB), Active Protein (Cat# AAA2105161)
Full Name
Active Creatine Kinase, Brain (CKB)
Gene Names
CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
Applications
Cell Culture, Activity Assays
Purity
> 95%
Activity Data
(Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse Arg and recombinant mouse UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Arginase (ARG), Active Protein (Cat# AAA2097288)
Full Name
Active Arginase (ARG)
Gene Names
Arg1; AI; PGIF; Arg-1; AI256583
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Sequence
Phospholipase A2, Lipoprotein Associated (LpPLA2), Active Protein (Cat# AAA2105125)
Full Name
Active Phospholipase A2, Lipoprotein Associated (LpPLA2)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays
Purity
>95%
Activity Data
(Figure. The binding activity of TK1 with GAPDH.Thymidine kinase 1 (TK1), is a human thymidine kinase. Thymidine kinase has been making a growing impact in the cancer research community. It has been found that elevated blood serum levels of TK-1 correlates with metastatic capabilities of the cancer and thereby can be used to detect malignant types of cancer, furthermore TK-1 has been found to show up in blood serum even before clinical symptoms even start to show. Besides, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) has been identified as an interactor of TK1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TK1 and recombinant human GAPDH. Briefly, TK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAPDH-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TK1 and GAPDH was shown in Figure 1, and this effect was in a dose dependent manner.)
Thymidine Kinase 1, Soluble (TK1), Active Protein (Cat# AAA2105175)
Full Name
Active Thymidine Kinase 1, Soluble (TK1)
Gene Names
TK1; TK2
Applications
Cell Culture, Activity Assays
Purity
> 95%
Thymidylate Synthetase (TYMS), Active Protein (Cat# AAA2105189)
Full Name
Active Thymidylate Synthetase (TYMS)
Gene Names
TYMS; TS; TMS; HST422
Applications
Cell Culture, Activity Assays
Purity
> 90%
Testing Data
(Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure 1.The binding activity of CTSV with RB1.)
Cathepsin V (CTSV), Active Protein (Cat# AAA2097309)
Full Name
Active Cathepsin V (CTSV)
Gene Names
CTSV; CTSU; CATL2; CTSL2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Activity Data
(Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant human MMP9 (1000ng, 500ng, 100ng, 10ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant human MMP9 (10-70kd) was shown in figure 1.)
Matrix Metalloproteinase 9 (MMP9), Active Protein (Cat# AAA2105102)
Full Name
Active Matrix Metalloproteinase 9 (MMP9)
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Applications
Cell Culture, Activity Assays
Purity
> 95%
Immunofluorescence (IF)
( Immunofluoresence staining of fixed HeLa cells with anti-TrkA antibody MNAC13 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of MNAC13 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
TrkA, Monoclonal Recombinant Antibody (Cat# AAA488320)
Full Name
Anti-TrkA [MNAC13]
Gene Names
NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
Reactivity
Human
Applications
EIA, SPR, BL
Purity
Protein A Affinity Purified
Sequence
Dipeptidyl Peptidase IV (DPP4), Active Protein (Cat# AAA2105126)
Full Name
Active Dipeptidyl Peptidase IV (DPP4)
Gene Names
DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Activity Data
(Figure. The binding activity of NOS2 with UCHL5.Nitric oxide synthase 2, inducible (NOS2) is a member of Nitric oxide synthases (NOSs) family. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of NOS2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NOS2 and recombinant mouse UCHL5. Briefly, NOS2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NOS2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NOS2 and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Nitric Oxide Synthase 2, Inducible (NOS2), Active Protein (Cat# AAA2105120)
Full Name
Active Nitric Oxide Synthase 2, Inducible (NOS2)
Gene Names
Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
Applications
Cell Culture, Activity Assays
Purity
> 97%
Sequence Information
Kinase R, Recombinant Protein (Cat# AAA2030210)
Full Name
Recombinant Protein Kinase R (PKR)
Reactivity
Human
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>80%
Typical Testing Data/Standard Curve (for reference only)
()
Phosphorylated adenosine monophosphate activated protein kinase, AMPK, ELISA Kit (Cat# AAA163800)
Full Name
Human Phosphorylated adenosine monophosphate activated protein kinase, AMPK ELISA Kit
Reactivity
Human
Activity Data
(Elastase 4 (ELA4) is a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. It regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ELA4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ELA4 and recombinant rat uPAR. Briefly, ELA4 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ELA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ELA4 and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.)
Elastase 4 (ELA4), Active Protein (Cat# AAA2097293)
Full Name
Active Elastase 4 (ELA4)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Sequence
Janus Kinase 3 (JAK3), Recombinant Protein (Cat# AAA2009482)
Full Name
Recombinant Janus Kinase 3 (JAK3)
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Applications
WB
Purity
>97%
Activity Data
(Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Estrogen Receptor Alpha (ERa) has been identified as an interactor of, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Arginase( Arg) and recombinant rat ERa. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ERa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and ERa was shown in Figure 1, and this effect was in a dose dependent manner.)
Arginase (ARG), Active Protein (Cat# AAA2097289)
Full Name
Active Arginase (ARG)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Sequence
Histone Deacetylase 1 (HDAC1), Active Protein (Cat# AAA2105171)
Full Name
Active Histone Deacetylase 1 (HDAC1)
Gene Names
HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays
Purity
>95%
Activity Data
(Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097301)
Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Immunohistochemistry (IHC)
( Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.)
erbB-2 (Her-2/neu), Recombinant Antibody (Cat# AAA488050)
Full Name
Anti-erbB-2 (Her-2/neu) [4D5-8 (trastuzumab)]
Reactivity
Human
Applications
EIA, FC/FACS, IHC
Purity
Protein A Affinity Purified
