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Gene Name
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3143 results for " Kinase Assays" - showing 200-250
Activity Data
(CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.)
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA2097353)
Full Name
Active Carbonic Anhydrase II (CA2)
Gene Names
Car2; Ca2
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Discoidin Domain Receptor 1, Monoclonal Recombinant Antibody (Cat# AAA488317)
Full Name
Anti-Discoidin Domain Receptor 1 [3E3]
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human
Applications
EIA, WB, BL
Purity
Protein A Affinity Purified
Activity Data
(Suppressor of cytokine signaling 3 (SOCS3) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family that negatively regulates cytokine signal transduction. SOCS3 is feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. SOCS3 also can bind to JAK2 kinase inhibits its kinase activity and nhibits insulin signaling in adipose tissue and the liver. Besides, Glycoprotein 130 (gp130) has been identified as an interactor of SOCS3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SOCS3 and recombinant mouse gp130. Briefly, SOCS3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SOCS3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SOCS3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of SOCS3 and gp130 was shown in Figure 1, and this effect was in a dose dependent manner.)
Suppressors Of Cytokine Signaling 3 (SOCS3), Active Protein (Cat# AAA2097298)
Full Name
Active Suppressors Of Cytokine Signaling 3 (SOCS3)
Gene Names
Socs3; Cis3; Ef10; Ssi3; Cish3; EF-10; SSI-3
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
Hsp90, Monoclonal Antibody (Cat# AAA800582)
Full Name
Hsp90 Antibody
Reactivity
Bovine, Chicken, Human, Mouse, Porcine (Pig), Rat, Rabbit
Applications
IP, IHC, WB, EIA, AM
Purity
Protein G Purified
Activity Data
(NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner.)
Neuraminidase (NEU), Active Protein (Cat# AAA2097248)
Full Name
Active Neuraminidase (NEU)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Activity Data
(JAK3 (Tyrosine-protein kinase JAK3) is a non-receptor tyrosine kinase that involved in various processes such as cell growth, development, or differentiation. Besides, STAM2 has been identified as an interactor of JAK3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK3 and recombinant human STAM2. Briefly, JAK3 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAM2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of JAK3 and STAM2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Janus Kinase 3 (JAK3), Active Protein (Cat# AAA2097317)
Full Name
Active Janus Kinase 3 (JAK3)
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Activity Data
(Figure. The binding activity of ODC with TK1.The enzyme ornithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. This reaction is the committed step in polyamine synthesis. Lysine 69 on ornithine decarboxylase (ODC) binds the cofactor pyridoxal phosphate to form a Schiff base. Ornithine displaces the lysine to form a Schiff base attached to ODC, which decarboxylates to form a quinoid intermediate. This intermediate rearranges to form a Schiff base attached to putrescine, which is attacked by lysine to release putrescine product and reform PLP-bound ODC. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ODC and recombinant rat TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105138)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
Odc1; Odc
Applications
Cell Culture, Activity Assays
Purity
> 97%
Activity Data
(Figure. The binding activity of TAT with GS.Tyrosine aminotransferase (TAT) is an enzyme present in the liver and catalyzes the conversion of tyrosine to 4-hydroxyphenylpyruvate. In humans, the tyrosine aminotransferase protein is encoded by the TAT gene. A deficiency of the enzyme in humans can result in what is known as Type II Tyrosinemia, wherein there is an abundance of tyrosine as a result of tyrosine failing to undergo an aminotransferase reaction to form 4-hydroxyphenylpyruvate. Besides, Glutamine synthetase (GS) has been identified as an interactor of TAT, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TAT and recombinant mouse GS. Briefly, TAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GS-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TATpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TAT and GS was shown in Figure 1, and this effect was in a dose dependent manner.)
Tyrosine Aminotransferase (TAT), Active Protein (Cat# AAA2105184)
Full Name
Active Tyrosine Aminotransferase (TAT)
Applications
Cell Culture, Activity Assays
Purity
> 95%
Typical Testing Data/Standard Curve (for reference only)
Arg, ELISA Kit (Cat# AAA2507239)
Full Name
Mouse Arg (Arginase) ELISA Kit
Gene Names
ABL2; ARG; ABLL
Reactivity
Mouse
Activity Data
(Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097302)
Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Activity Data
(NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner.)
Neuraminidase (NEU), Active Protein (Cat# AAA2097247)
Full Name
Active Neuraminidase (NEU)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Activity Data
(Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. Thus we have chosed casein-zymography to measure the activity of MMP13. Briefly, various concentrations of MMP13 (10ug, 5ug, 1ug, 0.1ug, 0.01ug) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat-polyacrylamide gel (SDS-PAGE; 15% gels) containing casein (1 mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze casein nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and trypsase (1ug/mL) as positive control.Result : Casein hydrolysis by recombinant human MMP13 was shown in figure 1.)
Matrix Metalloproteinase 13 (MMP13), Active Protein (Cat# AAA2105075)
Full Name
Active Matrix Metalloproteinase 13 (MMP13)
Gene Names
MMP13; CLG3; MDST; MANDP1; MMP-13
Applications
Cell Culture, Activity Assays
Purity
> 95%
Activity Data
(DGKe (Diacylglycerol kinase epsilon) is an enzyme that mainly involved in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) has been proven to be an interactor of DGKe through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human DGKe and recombinant human PDHA. Briefly, DGKe were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL DGKe were then transferred to PDHA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-DGKe pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of DGKe and PDHA was shown in Figure 1, and this effect was in a dose dependent manner.)
Diacylglycerol Kinase Epsilon (DGKe), Active Protein (Cat# AAA2097245)
Full Name
Active Diacylglycerol Kinase Epsilon (DGKe)
Gene Names
DGKE; DGK; AHUS7; DAGK5; DAGK6; NPHS7
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Activity Data
(Glutathione S-transferase Mu 1 (GSTM1) belongs to the mu class. GSTM1 can detoxifying carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Besides, uncoupling Protein 1 (UCP1) has been identified as an interactor of GSTM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GSTM1 and recombinant rat UCP1. Briefly, GSTM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCP1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GSTM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTM1 and UCP1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Glutathione S Transferase Mu 1 (GSTm1), Active Protein (Cat# AAA2097278)
Full Name
Active Glutathione S Transferase Mu 1 (GSTm1)
Gene Names
Gstm1; GSTA3
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Chromatin Immunoprecipitation (ChIP)
(Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.)
TAF9, Polyclonal Antibody (Cat# AAA3202129)
Full Name
TAF9 antibody - N-terminal region
Gene Names
AK6; CIP; CINAP; AD-004; hCINAP; CGI-137
Reactivity
Cow, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
IHC, WB, ChIP
Purity
Affinity Purified
Activity Data
(Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Arg and recombinant human UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Arginase (ARG), Active Protein (Cat# AAA2097287)
Full Name
Active Arginase (ARG)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Ephrin Type A receptor 2, Recombinant Antibody (Cat# AAA488192)
Full Name
Anti-Human Ephrin Type A receptor 2 [1C1]
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human
Applications
EIA, FC/FACS, WB, IF
Purity
Protein A Affinity Purified
Sequence
Elastase 2, Neutrophil, Active Protein (Cat# AAA2105086)
Full Name
Active Elastase 2, Neutrophil (ELA2)
Applications
Cell culture, Activity Assays
Purity
> 98%
Testing Data
ATM, Monoclonal Antibody (Cat# AAA4157759)
Full Name
Anti-ATM Protein Kinase Phospho-Ser1981 for WB, IF and IP Antibody
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Protein A Purified
Discoidin Domain Receptor 1, Monoclonal Recombinant Antibody (Cat# AAA488318)
Full Name
Anti-Discoidin Domain Receptor 1 [3E3]
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human
Applications
EIA, WB, BL
Purity
Protein A Affinity Purified
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-EGFR S3 12D Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of S3 12D at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
EGFR, Monoclonal Recombinant Antibody (Cat# AAA488322)
Full Name
Anti-EGFR [S3 12D]
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
EIA, IHC, WB, ICC, IF
Purity
Protein A Affinity Purified
Activity Data
(Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was shown in Figure 1, and this effect was in a dose dependent manner.)
Caspase 1 (CASP1), Active Protein (Cat# AAA2097294)
Full Name
Active Caspase 1 (CASP1)
Gene Names
Casp1; Ice; Il1bc
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Activity Data
(Figure 1. The binding activity of ANGPT2 withTie2.Angiopoietin is part of a family of vascular growth factors that play a role in embryonic and postnatal angiogenesis. Angiopoietin cytokines are involved with controlling microvascular permeability, vasodilation, and vasoconstriction by signaling smooth muscle cells surrounding vessels. There are now four identified angiopoietins: ANGPT1, ANGPT2, ANGPT3, ANGPT4. Angiopoietin 2 (ANGPT2) promotes cell death and disrupts vascularization. Yet, when it is in conjunction with vascular endothelial growth factors, or VEGF, it can promote neo-vascularization. Besides, TEK Tyrosine Kinase (Tie2) has been identified as an interactor of ANGPT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ANGPT2 and recombinant humanTie2. Briefly, ANGPT2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Tie2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ANGPT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ANGPT2 and Tie2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Angiopoietin 2 (ANGPT2), Active Protein (Cat# AAA2105045)
Full Name
Active Angiopoietin 2 (ANGPT2)
Gene Names
ANGPT2; ANG2; AGPT2
Applications
Cell Culture, Activity Assays
Purity
> 95%
EGFR, Recombinant Antibody (Cat# AAA488181)
Full Name
Anti-EGFR [C225 (Cetuximab)]
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Activity Data
(Figure. Cell proliferation of ECV-304 cells inhibit by VEGFR2.Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) also known as kinase insert domain receptor acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. VEGFR2 functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. Regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. To test the effect on inhibit the VEGF-dependent proliferation of endothelium cells, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1mug/mL Vascular Endothelial Growth Factor C (VEGFC) and various concentrations of recombinant human VEGFR2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of ECV-304 cells after incubation with VEGFR2 for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant VEGFR2 for 96h. The result was shown in Figure 2. It was obvious that VEGFR2 significantly inhibit cell viability of ECV-304.(A) ECV-304 cells cultured in DMEM, stimulated with 10ng/mL VEGFR2 for 96h; (B) Unstimulated ECV-304 cells cultured in DMEM for 96h.)
Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), Active Protein (Cat# AAA2105139)
Full Name
Active Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Applications
Cell Culture, Activity Assays
Purity
> 97%
EGFR, Monoclonal Recombinant Antibody (Cat# AAA488321)
Full Name
Anti-EGFR [S3 12D]
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
EIA, IHC, WB, ICC, IF
Purity
Protein A Affinity Purified
Testing Data
ATM, Monoclonal Antibody (Cat# AAA4157012)
Full Name
Anti-ATM Protein Kinase Phospho-Ser1981 for WB, IF and IP Antibody
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Protein A Purified
Chromatin Immunoprecipitation (ChIP)
(Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.)
PRKCG, Polyclonal Antibody (Cat# AAA3212763)
Full Name
PRKCG antibody - N-terminal region
Gene Names
PRKCG; PKCC; PKCG; SCA14; PKCI(3); PKCgamma; PKC-gamma
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
ChIP, WB
Purity
Affinity Purified
Structure
Testing Data
ATM, Monoclonal Antibody (Cat# AAA4156960)
Full Name
Anti-ATM Protein Kinase Phospho-Ser1981 for WB, IF and IP Antibody
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Protein A Purified
Activity Data
(Figure. The binding activity of ERK2 with PTPRJ.)
Extracellular Signal Regulated Kinase 2 (ERK2), Active Protein (Cat# AAA2105127)
Full Name
Active Extracellular Signal Regulated Kinase 2 (ERK2)
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
Applications
Cell Culture, Activity Assays
Purity
> 95%
Typical Testing Data/Standard Curve (for reference only)
Creatine Kinase MM isoenzyme (CK-MM), ELISA Kit (Cat# AAA931554)
Full Name
Human Creatine Kinase MM isoenzyme, CK-MM ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
2',5'-Oligoadenylate Synthetase 1 (OAS1), ELISA Kit (Cat# AAA2022869)
Full Name
Human 2',5'-Oligoadenylate Synthetase 1 (OAS1) ELISA Kit
Gene Names
OAS1Z; OAS1
Reactivity
Human
IL-1 BETA, Polyclonal Antibody (Cat# AAA220072)
Full Name
RABBIT ANTI CHICKEN INTERLEUKIN-1 BETA:Preservative Free
Gene Names
IL1B; IL1beta; IL-1BETA
Applications
EIA, FN, WB
Typical Testing Data/Standard Curve (for reference only)
Caspase 1 (CASP1), ELISA Kit (Cat# AAA2024439)
Full Name
Mouse Caspase 1 (CASP1) ELISA Kit
Gene Names
Casp1; Ice; Il1bc
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4), ELISA Kit (Cat# AAA2022801)
Full Name
Human A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) ELISA Kit
Gene Names
ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1), ELISA Kit (Cat# AAA2025514)
Full Name
Mouse 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit
Gene Names
HSD11B1; HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
mammalian target of rapamyein, mTOR, ELISA Kit (Cat# AAA700406)
Full Name
Human mammalian target of rapamyein, mTOR ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Phosphotylinosital 3 kinase, PI3K, ELISA Kit (Cat# AAA703766)
Full Name
Human Phosphotylinosital 3 kinase, PI3K ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Proteasome Subunit Alpha Type 1 (PSMa1), ELISA Kit (Cat# AAA2019957)
Full Name
Human Proteasome Subunit Alpha Type 1 (PSMa1) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Phosphotylinosital 3 kinase, PI3K, ELISA Kit (Cat# AAA702819)
Full Name
Rat Phosphotylinosital 3 kinase, PI3K ELISA Kit
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
bone morphogenetic protein receptor, type IA, ELISA Kit (Cat# AAA700462)
Full Name
Human Bone morphogenetic protein receptor 1A, BMPR-1A ELISA Kit
Gene Names
BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3), ELISA Kit (Cat# AAA2022145)
Full Name
Human 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Phosphorylated Adenosine Monophosphate Activated Protein Kinase, pAMPK, ELISA Kit (Cat# AAA705414)
Full Name
Mouse Phosphorylated Adenosine Monophosphate Activated Protein Kinase, pAMPK ELISA Kit
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
A Disintegrin And Metalloprotease 15 (ADAM15), ELISA Kit (Cat# AAA2022132)
Full Name
Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
A Disintegrin And Metalloprotease 10 (ADAM10), ELISA Kit (Cat# AAA2019893)
Full Name
Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit
Gene Names
ADAMTS10; WMS; WMS1; ADAM-TS10; ADAMTS-10
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
A Disintegrin And Metalloprotease 12 (ADAM12), ELISA Kit (Cat# AAA2023202)
Full Name
Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit
Gene Names
ADAMTS12; PRO4389
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Isocitrate Dehydrogenase 1, Soluble (IDH1), ELISA Kit (Cat# AAA2022319)
Full Name
Human Isocitrate Dehydrogenase 1, Soluble (IDH1) ELISA Kit
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Human
Tumor Type M2 Pyruvate Kinase (M2PK), Polyclonal Antibody (Cat# AAA340499)
Full Name
Tumor Type M2 Pyruvate Kinase (M2PK)
Applications
EIA, WB, DB, IHC
Typical Testing Data/Standard Curve
A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1), ELISA Kit (Cat# AAA2021525)
Full Name
Human A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) ELISA Kit
Reactivity
Human
