Human creatine kinase, muscle ELISA Kit | CKM elisa kit

Human Creatine kinase M-type, CKM ELISA Kit

Cat. Num. AAA930166

• Gene Names: CKM; CKMM; M-CK
• Reactivity: Human
• Synonyms: creatine kinase; muscle; Human Creatine kinase M-type; CKM ELISA Kit; Human Creatine kinase M-type (CKM) ELISA kit; CKMM; M-CK; creatine kinase M chain; creatine kinase-M; muscle creatine kinase; CKM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CKM. No significant cross-reactivity or interference between Human CKM and analogues was observed.
• Sequence Length: 381

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 3.12uU/ml-200 uU/ml
• Sensitivity: 1.56 uU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CKM elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CKM. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for CKM. The competitive inhibition reaction is launched between with pre-coated CKM and CKM in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of CKM in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21536288
• NCBI GeneID: 1158
• NCBI Accession #: NP_001815.2
• NCBI GenBank Nucleotide #: NM_001824.4
• UniProt Accession #: P06732; Q96QL9
• Molecular Weight: 43,101 Da
• NCBI Official Full Name: creatine kinase M-type
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: CKM
• NCBI Official Synonym Symbols: CKMM; M-CK
• NCBI Protein Information: creatine kinase M-type; creatine kinase M chain
• UniProt Protein Name: Creatine kinase M-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKM
• UniProt Synonym Gene Names: CKMM
• UniProt Entry Name: KCRM_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Uniprot Description

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Kinase, other; Amino Acid Metabolism - arginine and proline; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation