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3143 results for " Kinase Assays" - showing 100-150
Glycosaminoglycan xylosylkinase, ELISA Kit (Cat# AAA282231)
Full Name
Human Glycosaminoglycan xylosylkinase (FAM20B) ELISA Kit
Gene Names
FAM20B; gxk1
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor), ELISA Kit (Cat# AAA703785)
Full Name
Human vascular endothelial cell growth factor receptor 1, VEGFR-1/Flt1 ELISA Kit
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Mannose Associated Serine Protease 1 (MASP1), ELISA Kit (Cat# AAA2018944)
Full Name
Human Mannose Associated Serine Protease 1 (MASP1) ELISA Kit
Gene Names
MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
growth arrest-specific 6, ELISA Kit (Cat# AAA906622)
Full Name
Human Growth arrest-specific protein 6, GAS6 ELISA Kit
Gene Names
GAS6; AXSF; AXLLG
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Matrix Metalloproteinase 14 (MMP14), ELISA Kit (Cat# AAA2022640)
Full Name
Human Matrix Metalloproteinase 14 (MMP14) ELISA Kit
Gene Names
MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Ephrin B1 (EFNB1), ELISA Kit (Cat# AAA2020110)
Full Name
Human Ephrin B1 (EFNB1) ELISA Kit
Gene Names
EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
Reactivity
Human
Activity Data
(Figure. The binding activity of SPC with EIF2aK3.Surfactant associated proteins (SPC), is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SPC and recombinant mouse EIF2aK3. Briefly, SPC were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EIF2aK3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SPC and EIF2aK3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Surfactant Associated Protein C (SPC), Active Protein (Cat# AAA2105144)
Full Name
Active Surfactant Associated Protein C (SPC)
Gene Names
Sftpc; SP5; SPC; SP-C; Sftp2; Bricd6; Sftp-2; pro-SpC
Applications
Cell Culture, Activity Assays
Purity
> 97%
Activity Data
(Anti-Müllerian hormone (AMH), also named Müllerian inhibiting substance (MIS) belongs to a tissue-specific TGF-beta superfamily growth factor. It can be expressed by male sertoli cells and postnatal testis, and ovarian granulosa cells of females postpartum. AMH expression is critical to sex differentiation at a specific time during fetal development, it appears to be tightly regulated by SF1, GATA factors, DAX1 and FSH. AMH signals through a characteristic receptor consisting of a type I and a type II receptor serine/threonine kinase. Especially the type II receptor is unique and specific receptor for AMH. Besides, Mothers Against Decapentaplegic Homolog 9 (Smad9) has been identified as an interactor of AMH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AMH and recombinant human (Smad9) Briefly, AMH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Smad9-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AMH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of AMH and Smad9 was shown in Figure 1, and this effect was in a dose dependent manner.)
Anti-Mullerian Hormone (AMH), Active Protein (Cat# AAA2097270)
Full Name
Active Anti-Mullerian Hormone (AMH)
Gene Names
AMH; MIF; MIS
Reactivity
Homo sapiens (Human)
Applications
Cell culture, Activity Assays.
Purity
>95%
Typical Testing Data/Standard Curve (for reference only)
fms-related tyrosine kinase 4, ELISA Kit (Cat# AAA703153)
Full Name
Human Vascuar endothelial cell growth factor receptor 3, VEGFR-3/Flt-4 ELISA Kit
Gene Names
FLT4; PCL; FLT41; LMPH1A; VEGFR3
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
kinase insert domain receptor (a type III receptor tyrosine kinase), ELISA Kit (Cat# AAA702919)
Full Name
Mouse Vascular endothelial cell growth factor receptor 2, VEGFR-2/Flk-1 ELISA Kit
Gene Names
Kdr; Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
Carbonic Anhydrase IX (CA9), ELISA Kit (Cat# AAA2019423)
Full Name
Human Carbonic Anhydrase IX (CA9) ELISA Kit
Gene Names
CA9; MN; CAIX
Reactivity
Human
Typical Standard Curve/Testing Data
Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz), ELISA Kit (Cat# AAA2025590)
Full Name
Human Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Amylase Alpha 1, Salivary (AMY1), ELISA Kit (Cat# AAA2021183)
Full Name
Human Amylase Alpha 1, Salivary (AMY1) ELISA Kit
Reactivity
Human
Sequence
Matrix Metalloproteinase 9 (MMP9), Active Protein (Cat# AAA2105103)
Full Name
Active Matrix Metalloproteinase 9 (MMP9)
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Reactivity
Sus scrofa; Porcine (Pig)
Applications
Cell culture; Activity Assays.
Purity
>94%
Typical Testing Data/Standard Curve (for reference only)
Phosphorylated adenosine monophosphate activated protein kinase, AMPK, ELISA Kit (Cat# AAA164089)
Full Name
Rat Phosphorylated adenosine monophosphate activated protein kinase, AMPK ELISA Kit
Reactivity
Rat
Typical Standard Curve/Testing Data
Angiotensin I Converting Enzyme (ACE), ELISA Kit (Cat# AAA2021446)
Full Name
Human Angiotensin I Converting Enzyme (ACE) ELISA Kit
Gene Names
ACE; DCP; ICH; ACE1; DCP1; CD143; MVCD3
Reactivity
Human
growth arrest-specific 6, ELISA Kit (Cat# AAA939560)
Full Name
Mouse growth arrest-specific gene-6, gas-6 ELISA Kit
Gene Names
Gas6; Gas-6
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
PDK4, ELISA Kit (Cat# AAA2512160)
Full Name
Human PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4)ELISA Kit
Reactivity
Human
Activity Data
(Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.)
Arylsulfatase B (ARSB), Active Protein (Cat# AAA2097318)
Full Name
Active Arylsulfatase B (ARSB)
Gene Names
ARSB; ASB; G4S; MPS6
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 80%
Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha, ELISA Kit (Cat# AAA282248)
Full Name
Human Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha (PIP4K2A) ELISA Kit
Gene Names
PIP4K2A; PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
FLT1/VEGFR1, ELISA Kit (Cat# AAA2511664)
Full Name
Rat FLT1/VEGFR1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
CDK7, ELISA Kit (Cat# AAA2504448)
Full Name
Human CDK7 (Cyclin Dependent Kinase 7) ELISA Kit
Gene Names
CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
Reactivity
Human
Activity Data
(Figure 1. Activity DataActivity: Hexosaminidase A Alpha (HEXa) is a lysosomal enzyme. There are three predominant isoenzymes: hexosaminidase A, B and S. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines. The enzymes are composed of two alpha and/or beta subunits, which are coded by HEXA and HEXB genes, respectively. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit which contains a key residue, Arg-424 is able to hydrolyze GM2 gangliosides. Hexosaminidase A (alpha polypeptide) plays a critical role in the brain and spinal cord (central nervous system). Besides, Hexosaminidase B Beta (HEXB) has been identified as an interactor of HEXA, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse HEXA and recombinant mouse HEXB. Briefly, HEXA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HEXB-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-HEXA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50DL stop solution to the wells and read at 450nm immediately. The binding activity of of HEXA and HEXB was shown in Figure 1, and this effect was in a dose dependent manner.)
Hexosaminidase A Alpha (HEXa), Active Protein (Cat# AAA2097268)
Full Name
Active Hexosaminidase A Alpha (HEXa)
Gene Names
Hexa; Hex-1
Applications
Cell culture, Activity Assays
Purity
>98%
Typical Testing Data/Standard Curve (for reference only)
Protein Kinase C Epsilon (PKCe), ELISA Kit (Cat# AAA2701804)
Full Name
Human Protein Kinase C Epsilon (PKCe) ELISA Kit
Gene Names
PRKCE; PKCE; nPKC-epsilon
Reactivity
Human
Sequence
Carboxylesterase 1 (CES1), Active Protein (Cat# AAA2105173)
Full Name
Active Carboxylesterase 1 (CES1)
Gene Names
CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays
Purity
>90%
Typical Testing Data/Standard Curve (for reference only)
Matrix Metalloproteinase 9 (MMP9), ELISA Kit (Cat# AAA2018844)
Full Name
Rat Matrix Metalloproteinase 9 (MMP9) ELISA Kit
Reactivity
Rat
Typical Testing Data/Standard Curve (for reference only)
Pim-1 Oncogene (PIM1), ELISA Kit (Cat# AAA2704541)
Full Name
Human Pim-1 Oncogene (PIM1) ELISA Kit
Gene Names
PIM1; PIM
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
STK, ELISA Kit (Cat# AAA2510204)
Full Name
Mouse STK (serine/threonine protein kinase) ELISA Kit
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
Nitric Oxide Synthase 2, Inducible (NOS2), ELISA Kit (Cat# AAA2024333)
Full Name
Rat Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit
Reactivity
Rat
Calmodulin, ELISA Kit (Cat# AAA280185)
Full Name
Plant Calmodulin (CAM) ELISA Kit
Gene Names
CALM1; caM; CAMI; PHKD; CPVT4; DD132; CALML2
Reactivity
Plant
Typical Testing Data/Standard Curve (for reference only)
SYK, ELISA Kit (Cat# AAA2506217)
Full Name
Human SYK (Spleen Tyrosine Kinase) ELISA Kit
Gene Names
Syk; Sykb
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Superoxide Dismutase 1, Soluble (SOD1), ELISA Kit (Cat# AAA2019556)
Full Name
Human Superoxide Dismutase 1, Soluble (SOD1) ELISA Kit
Reactivity
Human
Activity Data
(ENDOG (Endonuclease G) is a mitochondrial enzyme that cleaves DNA at double-stranded (DG)n. (DC)n and at single-stranded (DC)n tracts. This protein also has ribonuclease (RNase) and RNase H activities. It has been reported that EndoG forms complexes with some other proteins, including HSP70 (Heat shock 70 kDa protein). Besides, mouse HSPA1A (Heat shock 70 kDa protein 1A) shares similarities with rat HSP1A1 in amino acid sequence with the identity of 98.44%. Thus, a binding ELISA assay was conducted to detect the association of recombinant rat ENDOG with recombinant mouse HSPA1A. Briefly, ENDOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP1A1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ENDOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ENDOG with HSP1A1 was shown in Figure 1 and this effect was in a dose dependent manner.)
Endonuclease G, Mitochondrial (ENDOG), Active Protein (Cat# AAA2097242)
Full Name
Active Endonuclease G, Mitochondrial (ENDOG)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Activity Data
(Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Adenine Phosphoribosyltransferase (APRT), Active Protein (Cat# AAA2097303)
Full Name
Active Adenine Phosphoribosyltransferase (APRT)
Gene Names
APRT; AMP; APRTD
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Typical Testing Data/Standard Curve (for reference only)
Pyruvate Kinase, Muscle (PKM2), ELISA Kit (Cat# AAA2702096)
Full Name
Human Pyruvate Kinase, Muscle (PKM2) ELISA Kit
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
FGFR3, ELISA Kit (Cat# AAA2514844)
Full Name
Human FGFR3 (Fibroblast Growth Factor Receptor 3) ELISA Kit
Gene Names
FGFR3; ACH; CEK2; JTK4; CD333; HSFGFR3EX
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Lipase, Lipoprotein (LIPD), ELISA Kit (Cat# AAA2019970)
Full Name
Human Lipase, Lipoprotein (LIPD) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
VEGFR3/Flt4, ELISA Kit (Cat# AAA2513533)
Full Name
Human VEGFR3/Flt4 (Vascular Endothelial Cell Growth Factor Receptor 3) ELISA Kit
Gene Names
FLT4; PCL; FLT41; LMPH1A; VEGFR3
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
MLCK/MYLK, ELISA Kit (Cat# AAA2515928)
Full Name
Mouse MLCK/MYLK(Myosin light chain kinase)ELISA Kit
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
CDK9, ELISA Kit (Cat# AAA2501429)
Full Name
Mouse CDK9 (Cyclin Dependent Kinase 9) ELISA Kit
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Mouse
Typical Testing Data/Standard Curve (for reference only)
ARG1, ELISA Kit (Cat# AAA2509985)
Full Name
Human ARG1 (Arginase I) ELISA Kit
Gene Names
ABL2; ARG; ABLL
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
CSNK2A2, ELISA Kit (Cat# AAA2516130)
Full Name
Human CSNK2A2 (Casein Kinase 2, alpha 2 Polypeptide) ELISA Kit
Gene Names
CSNK2A2; CK2A2; CSNK2A1
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
EphA2, ELISA Kit (Cat# AAA2511581)
Full Name
Human EphA2 (Tyrosine protein kinase receptor A2) ELISA Kit
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Enolase, Neuron Specific (NSE), ELISA Kit (Cat# AAA2022532)
Full Name
Human Enolase, Neuron Specific (NSE) ELISA Kit
Reactivity
Human
Activity Data
(Figure. The binding activity of ACE with ACTb.Angiotensin-converting enzyme ACE, is a central component of the renin-angiotensin system (RAS), which controls blood pressure by regulating the volume of fluids in the body. It converts the hormone angiotensin I to the active vasoconstrictor angiotensin II. Therefore, ACE indirectly increases blood pressure by causing blood vessels to constrict. ACE inhibitors are widely used as pharmaceutical drugs for treatment of cardiovascular diseases. Besides, Actin Beta (ACTb) has been identified as an interactor of ACE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ACE and recombinant human ACTb. Briefly, ACE were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ACE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ACE and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Angiotensin I Converting Enzyme (ACE), Active Protein (Cat# AAA2105044)
Full Name
Active Angiotensin I Converting Enzyme (ACE)
Gene Names
ACE; DCP; ACE1; DCP1; CD143
Applications
Cell Culture, Activity Assays
Purity
> 90%
Activity Data
(JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.)
Janus Kinase 2 (JAK2), Active Protein (Cat# AAA2097244)
Full Name
Active Janus Kinase 2 (JAK2)
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Activity Data
(Figure. The binding activity of ODC with TK1.Ornithine decarboxylase (ODC) is an enzyme can catalyze the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. The ornithine decarboxylation reaction catalyzed by ornithine decarboxylase is the first and committed step in the synthesis of polyamines, particularly putrescine, spermidine and spermine. Polyamines are important for stabilizing DNA structure, the DNA double strand-break repair pathway and as antioxidants. Therefore, ornithine decarboxylase is an essential enzyme for cell growth, producing the polyamines necessary to stabilize newly synthesized DNA. Lack of ODC causes cell apoptosis in embryonic mice, induced by DNA damage. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ODC and recombinant human TK1. Briefly, ODC were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105136)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
ODC1; ODC
Applications
Cell Culture, Activity Assays
Purity
> 90%
Typical Testing Data/Standard Curve (for reference only)
EFNA5, ELISA Kit (Cat# AAA2500291)
Full Name
Human EFNA5 (Ephrin A5) ELISA Kit
Gene Names
EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
Aldose Reductase Like Protein 1 (ARL1), ELISA Kit (Cat# AAA2023383)
Full Name
Human Aldose Reductase Like Protein 1 (ARL1) ELISA Kit
Reactivity
Human
Typical Testing Data/Standard Curve (for reference only)
CKM, ELISA Kit (Cat# AAA2503308)
Full Name
Human CKM (Creatine Kinase, Muscle) ELISA Kit
Reactivity
Human
