Immunohistochemistry (IHC)
( Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.)
erbB-2 (Her-2/neu) Recombinant Antibody
Anti-erbB-2 (Her-2/neu) [4D5-8 (trastuzumab)]
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
erbB-2 (Her-2/neu); Anti-erbB-2 (Her-2/neu) [4D5-8 (trastuzumab)]; CD340; Metastatic lymph node gene 19 protein (MLN 19); Protooncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; p185HER2; EGFR; erbB-2 (Her-2/neu) recombinant antibody
Reactivity
Human
Isotype
Rabbit IgG, kappa
Clone Number
4D5-8 (trastuzumab)
Specificity
Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30% of invasive breast cancers and 70% of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS only with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for erbB-2 (Her-2/neu) recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007).
Immunogen
A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunohistochemistry (IHC)
( Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.)
Related Product Information for erbB-2 (Her-2/neu) recombinant antibody
Recombinant monoclonal antibody to erbB-2 (Her-2/neu). Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody 4D5-8 (trastuzumab). This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for erbB-2 (Her-2/neu) recombinant antibody
References
Carter P, Presta L, Gorman CM, Ridgway JB, Henner D, Wong WL, Rowland AM, Kotts C, Carver ME, Shepard HM. Humanization of an anti-p185HER2 antibody for human cancer therapy. Proc Natl Acad Sci U S A. 1992 May 15; 89(10):4285-9. PMID:1350088
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The erbB-2 (Her-2/neu) (Catalog #AAA488050) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-erbB-2 (Her-2/neu) [4D5-8 (trastuzumab)] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's erbB-2 (Her-2/neu) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007). Researchers should empirically determine the suitability of the erbB-2 (Her-2/neu) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "erbB-2 (Her-2/neu), Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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