Western Blot (WB)
(Western blot analysis of p47 phox in lysates of COS7 , using p47 phox Antibody.)
Rabbit p47 phox Polyclonal Antibody | anti-p47phox antibody
p47 phox Antibody
Predicted: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken
Predicted: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of p47 phox in lysates of COS7 , using p47 phox Antibody.)
Western Blot (WB)
(Western blot analysis of p47 phox in lysates of COS7 , using p47 phox Antibody.)
Post Translational Modifications: Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.
Subunit Structure: Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain). Interacts with NCF4. Interacts with CYBB. Interacts (via the second SH3 domain) with CYBA. Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG. Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (By similarity).
Similarity: The PX domain mediates interaction with phosphatidylinositol 3,4-bisphosphate and other anionic phospholipids. In the autoinhibited, unphosphorylated state an intramolecular interaction with the C-terminal SH3 domain precludes phospholipid binding and interaction with CYBA. Phosphorylation disrupts the autoinhibited state.
NCBI and Uniprot Product Information
Predicted: 45 kDa
NCBI Description
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]
Uniprot Description
p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.
Protein type: EC 1.-.-.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: Golgi apparatus; extrinsic to membrane; rough endoplasmic reticulum; cell soma; dendrite; cytosol; NADPH oxidase complex
Molecular Function: protein binding; electron carrier activity; superoxide-generating NADPH oxidase activity; phosphatidylinositol-3,4-bisphosphate binding; SH3 domain binding; phosphoinositide binding
Biological Process: respiratory burst during defense response; respiratory burst; interaction with host; apoptosis; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; leukotriene metabolic process; cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of smooth muscle contraction; antigen processing and presentation of exogenous peptide antigen via MHC class I; cellular defense response; innate immune response; response to yeast; vascular endothelial growth factor receptor signaling pathway; protein targeting to membrane; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process
Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I
Research Articles on p47phox
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Product Notes
The p47phox ncf1 (Catalog #AAA9611015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p47 phox Antibody reacts with Human Predicted: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's p47 phox can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the p47phox ncf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p47 phox, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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