Rabbit anti-Human HCLS1 Polyclonal Antibody | anti-HCLS1 antibody

HCLS1, CT (HCLS1, HS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75) (AP)

Cat. Num. AAA6305801

• Gene Names: HCLS1; HS1; CTTNL
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HCLS1; Polyclonal Antibody; CT (HCLS1; HS1; Hematopoietic lineage cell-specific protein; Hematopoietic cell-specific LYN substrate 1; LckBP1; p75) (AP); p75; anti-HCLS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HCLS1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HCLS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-391 amino acids from the C-terminal region of human HCLS1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HCLS1 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HCLS1 antibody detected the HCLS1 protein (arrow).)

Immunohistochemistry (IHC)

(HCLS1 Antibody (C-term) (AP14038b)immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HCLS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HCLS1 antibody

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Product Categories/Family for anti-HCLS1 antibody

Antibodies; Proteins

References

Muzio, M., et al. Leukemia 21(9):2067-2070(2007). Lim, J., et al. Cell 125(4):801-814(2006). Hao, J.J., et al. J. Biol. Chem. 280(45):37988-37994(2005). Tao, W.A., et al. Nat. Methods 2(8):591-598(2005). Brunati, A.M., et al. J. Biol. Chem. 280(22):21029-21035(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 167234422
• NCBI GeneID: 3059
• NCBI Accession #: NP_005326.2
• NCBI GenBank Nucleotide #: NM_005335.4
• UniProt Accession #: P14317; Q53Y93; Q6IBK9; Q9UDK0
• Molecular Weight: 54,014 Da
• NCBI Official Full Name: hematopoietic lineage cell-specific protein
• NCBI Official Synonym Full Names: hematopoietic cell-specific Lyn substrate 1
• NCBI Official Symbol: HCLS1
• NCBI Official Synonym Symbols: HS1; CTTNL
• NCBI Protein Information: hematopoietic lineage cell-specific protein; p75; lckBP1
• UniProt Protein Name: Hematopoietic lineage cell-specific protein
• Protein Family: HCLS1-binding protein
• UniProt Gene Name: HCLS1
• UniProt Synonym Gene Names: HS1
• UniProt Entry Name: HCLS1_HUMAN

Uniprot Description

HS1: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: transcription factor complex; membrane; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; protein complex binding; actin binding; protein kinase binding; SH3 domain binding

Biological Process: positive regulation of granulocyte differentiation; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of actin filament polymerization; regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of STAT protein; actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation