Activity Data
(Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.)
Arylsulfatase B (ARSB) Active Protein | ARSB active protein
Active Arylsulfatase B (ARSB)
Activity Data
(Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.)
SDS-Page
SDS-Page
Testing Data
Testing Data
NCBI and Uniprot Product Information
Accurate Molecular Weight: 55kDa
NCBI Description
Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]
Uniprot Description
ARSB: Defects in ARSB are the cause of mucopolysaccharidosis type 6 (MPS6); also known as Maroteaux-Lamy syndrome. MPS6 is an autosomal recessive lysosomal storage disease characterized by intracellular accumulation of dermatan sulfate. Clinical features can include abnormal growth, short stature, stiff joints, skeletal malformations, corneal clouding, hepatosplenomegaly, and cardiac abnormalities. A wide variation in clinical severity is observed. Arylsulfatase B activity is defective in multiple sulfatase deficiency (MSD). A clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Arylsulfatase B activity is impaired in multiple sulfatase deficiency due to mutations in SUMF1. SUMF1 mutations result in defective post-translational modification of ARSB at residue Cys- 91 that is not converted to 3-oxoalanine. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.6.12; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase
Chromosomal Location of Human Ortholog: 5q14.1
Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; Golgi apparatus; lysosomal lumen; lysosome; mitochondrion; rough endoplasmic reticulum
Molecular Function: arylsulfatase activity; metal ion binding; N-acetylgalactosamine-4-sulfatase activity
Biological Process: autophagy; central nervous system development; chondroitin sulfate catabolic process; glycosphingolipid metabolic process; lysosomal transport; lysosome organization and biogenesis; neutrophil degranulation; post-translational protein modification; response to estrogen; response to methylmercury; response to nutrient; response to pH
Disease: Mucopolysaccharidosis Type Vi
Research Articles on ARSB
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Product Notes
The ARSB arsb (Catalog #AAA2097318) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Active Arylsulfatase B (ARSB) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Arylsulfatase B (ARSB) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the ARSB arsb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arylsulfatase B (ARSB), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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