Human ARG1 ELISA Kit | ARG1 elisa kit

Human ARG1 (Arginase I) ELISA Kit

Cat. Num. AAA2509985

• Gene Names: ABL2; ARG; ABLL
• Reactivity: Human
• Synonyms: ARG1; Human ARG1 (Arginase I) ELISA Kit; ARG1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human ARG1 in samples. No significant cross-reactivity or interference between Human ARG1 and analogues was observed.
• Sequence Length: 1043

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.13-200ng/mL
• Sensitivity: 1.88ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ARG1 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ARG1 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARG1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ARG1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ARG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ARG1. You can calculate the concentration of Human ARG1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 269847793
• NCBI GeneID: 27
• NCBI Accession #: NP_001161711.1
• NCBI GenBank Nucleotide #: NM_001168239.1
• UniProt Accession #: P42684; Q5T0X6; Q5W0C5; Q6NZY6; Q7Z301; A0M8X0; B7UEF2; B7UEF3; B7UEF4; B7UEF5
• Molecular Weight: 60,864 Da
• NCBI Official Full Name: Abelson tyrosine-protein kinase 2 isoform i
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: Abelson tyrosine-protein kinase 2; abelson-related gene protein; c-abl oncogene 2, non-receptor tyrosine kinase; tyrosine-protein kinase ARG; v-abl Abelson murine leukemia viral oncogene homolog 2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Arginase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Oncoprotein; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extrinsic to internal side of plasma membrane; lamellipodium; dendritic spine; cytosol; actin cytoskeleton; phagocytic cup

Molecular Function: actin monomer binding; actin filament binding; protein binding; manganese ion binding; protein-tyrosine kinase activity; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; positive regulation of protein binding; cerebellum morphogenesis; alpha-beta T cell differentiation; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of apoptosis; Bergmann glial cell differentiation; elevation of cytosolic calcium ion concentration; regulation of endocytosis; visual learning; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; actin filament bundle formation; cell migration; positive regulation of I-kappaB kinase/NF-kappaB cascade; dendrite morphogenesis; protein modification process; substrate-bound cell migration, cell extension; phagocytosis; regulation of cell proliferation; negative regulation of Rho protein signal transduction; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; neuron remodeling