Sequence
Carboxylesterase 1 (CES1) Active Protein | CES1 active protein
Active Carboxylesterase 1 (CES1)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
SDS-PAGE
(SDS-PAGESample: Active recombinant human, CES1 )
SDS-PAGE
(SDS-PAGESample: Active recombinant human, CES1 )
Western Blot (WB)
(Western BlotSample: Recombinant human, CES1Antibody: Rabbit Anti-CES1 human Ab (MBS2032568))
Western Blot (WB)
(Western BlotSample: Recombinant human, CES1Antibody: Rabbit Anti-CES1 human Ab (MBS2032568))
Activity Data
(The standard curve of 4-Nitrophenol.Carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50mM Tris, pH 7.5 (Assay Buffer), initiated by addition 50uL of various concentrations of CES1 (dilute by assay buffer) to 50uL of 2mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400nm.One unit of enzyme activity is defined as the 1ug of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant human CES1 is 1396 pmol/min/ug.Specific Activity (pmol/min/ug) = ΔOD*F/T*NΔOD=Adjusted for Substrate BlankF=Conversion Factor(convert from standard curve of 4-Nitrophenol)T=TimeN=Amount of enzyme)
Activity Data
(The standard curve of 4-Nitrophenol.Carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50mM Tris, pH 7.5 (Assay Buffer), initiated by addition 50uL of various concentrations of CES1 (dilute by assay buffer) to 50uL of 2mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400nm.One unit of enzyme activity is defined as the 1ug of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant human CES1 is 1396 pmol/min/ug.Specific Activity (pmol/min/ug) = ΔOD*F/T*NΔOD=Adjusted for Substrate BlankF=Conversion Factor(convert from standard curve of 4-Nitrophenol)T=TimeN=Amount of enzyme)
NCBI and Uniprot Product Information
Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Uniprot Description
CES1: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.1.1.56; EC 3.1.1.1
Chromosomal Location of Human Ortholog: 16q22.2
Cellular Component: extracellular space; endoplasmic reticulum lumen
Molecular Function: methylumbelliferyl-acetate deacetylase activity
Biological Process: epithelial cell differentiation; metabolic process; response to toxin
Research Articles on CES1
Similar Products
Product Notes
The CES1 ces1 (Catalog #AAA2105173) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Carboxylesterase 1 (CES1) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Carboxylesterase 1 (CES1) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the CES1 ces1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carboxylesterase 1 (CES1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
