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6202 results for " All Proteins" - showing 1400-1450


SDS-Page (SDS-PAGE Analysis Purified Coagulation Factor VII Mouse Monoclonal Antibody (F7/3511). Confirmation of Integrity and Purity of Antibody.)

VII/F7, Monoclonal Antibody (Cat# AAA4381658)

Full Name
Coagulation Factor VII/F7
Gene Names
F7; SPCA
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
SDS-Page (SDS-PAGE Analysis Purified CD31 Mouse Monoclonal Antibody (PECAM1/3525). Confirmation of Integrity and Purity of Antibody.)

CD31/PECAM-1, Monoclonal Antibody (Cat# AAA4381574)

Full Name
CD31/PECAM-1 (Endothelial Cell Marker)
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Fig.1. WB experiment of beta-Actin expression, the samples are Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3) each 10 ug, the primary antibody is beta-Actin mouse monoclonal antibody ( 1C7) (1:5000), the secondary antibody is goat anti-mouse IgG (1: 10000). )

Universal Loading Control Antibody Cocktail, Loading Control Kit (Cat# AAA9719226)

Full Name
Universal Loading Control Antibody Cocktail
Applications
WB
Pricing
$160/1 Kit | $720/5x1 Kit

Saccharomyces cerevisiae, Polyclonal Antibody (Cat# AAA315823)

Full Name
Rabbit anti S. cerevisiae
Applications
Western Blot
Purity
>95% pure. Protein A chromatography
Pricing
$530/1 mL | $2,235/5x1 mL

Gram Positive Bacteria, Monoclonal Antibody (Cat# AAA310234)

Full Name
MAb to Gram Positive Bacteria
Applications
EIA/ELISA, Immunofluorescence Assay and Colloidal Gold Conjugate
Purity
>90% pure. Protein A chromatography
Pricing
$870/1 mg | $3,760/5x1 mg

Gram Positive Bacteria, Monoclonal Antibody (Cat# AAA310317)

Full Name
MAb to Gram Positive Bacteria
Applications
ELISA, IFA and Colloidal Gold Conjugates
Purity
>90% pure. Protein A chromatography
Pricing
$870/1 mg | $3,760/5x1 mg

Treponema pallidum (Syphilis) (all antigens), Polyclonal Antibody (Cat# AAA315089)

Full Name
Rabbit anti Treponema pallidum
Applications
Immunofluorescence Assay, Immunohistochemistry - Paraffin-embedded sections
Purity
>95% pure. Sodium sulfate precipitation and DEAE chromatography
Pricing
$530/1 mL | $2,235/5x1 mL

PHAP (NT), Polyclonal Antibody (Cat# AAA194326)

Full Name
PHAP (NT) Polyclonal Antibody
Applications
Western Blot
Pricing
$475/0.1 mg | $2,090/5x0.1 mg

E. coli (O & K antigens), Polyclonal Antibody (Cat# AAA315044)

Full Name
Goat A' E. coli (all antigens)
Applications
EIA/ELISA, Immunofluorescence Assay
Purity
>95% pure. Sodium sulfate precipitation and ion-exchange chromatography
Pricing
$505/1 mL | $2,130/5x1 mL
Western Blot (WB) (MBS421389 (1ug/ml) staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

RARA, Antibody (Cat# AAA421389)

Full Name
Goat anti-RARA Antibody
Gene Names
RARA; RAR; NR1B1
Reactivity
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
EIA, WB, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Immunohistochemistry (IHC) ((4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

PIWI/HIWI, Polyclonal Antibody (Cat# AAA420816)

Full Name
Goat anti-PIWI/HIWI Antibody
Gene Names
PIWIL1; HIWI; MIWI; PIWI; CT80.1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Applications
EIA, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg

Mycobacterium tuberculosis (all antigens), Polyclonal Antibody (Cat# AAA315163)

Full Name
Rabbit A' M. tuberculosis
Applications
Immunohistochemistry - Paraffin; IFA
Purity
>95% pure. Protein A chromatography
Pricing
$530/1 mL | $2,235/5x1 mL

E. coli (O & K antigens), Polyclonal Antibody (Cat# AAA315027)

Full Name
Rabbit anti E. coli all ag's
Applications
EIA/ELISA, Immunofluorescence Assay
Purity
>95% pure. Protein A chromatography
Pricing
$530/1 mL | $2,235/5x1 mL
Western Blot (WB) ()

14-3-3 beta, Monoclonal Antibody (Cat# AAA834603)

Full Name
14-3-3 beta antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Applications
WB
Purity
14-3-3 beta antibody was purified by ammonium sulfate precipitation
Western Blot (WB) (All lanes : Anti-14-3-3 gamma Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

14-3-3 gamma, Polyclonal Antibody (Cat# AAA9211774)

Full Name
14-3-3 gamma Antibody
Gene Names
YWHAG; PPP1R170; 14-3-3GAMMA
Reactivity
Human, mouse, rat
Applications
WB, EIA, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.1 mL | $1,585/5x0.1 mL

S. cerevisiae, Antibody (Cat# AAA324221)

Full Name
Saccharomyces cerevisiae
Applications
WB, IHC
Purity
> 95%
Pricing
$310/1 mL | $1,345/5x1 mL

Artemin, Active Protein (Cat# AAA539358)

Full Name
Artemin protein
Gene Names
ARTN; EVN; NBN; ENOVIN
Applications
User optimized
Purity
> 98% pure
Pricing
$425/0.02 mg | $1,905/5x0.02 mg

Salmonella species (A,B,C,D,E,F & G Groups), Monoclonal Antibody (Cat# AAA312398)

Full Name
MAb to Salmonella species
Applications
EIA/ELISA, Immunofluorescence Assay, Immunoassay Antibody Pairs
Purity
>90% pure. Protein A chromatography
Pricing
$945/1 mg | $4,095/5x1 mg

Neisseria gonorrhoeae (all antigens), Polyclonal Antibody (Cat# AAA315050)

Full Name
Rabbit A' Neisseria gonorrhoeae
Applications
EIA/ELISA, Immunofluorescence Assay
Purity
>95% pure. Protein A chromatography.
Pricing
$530/1 mL | $2,235/5x1 mL

Serum, Secondary Antibody (Cat# AAA539256)

Full Name
Rabbit anti Pig Serum
Reactivity
Pig
Applications
User optimized
Pricing
$380/2 mL | $1,710/5x2 mL

Rotavirus NCDV (all antigens), Polyclonal Antibody (Cat# AAA315090)

Full Name
Goat anti Rotavirus NCDV
Applications
Immunofluorescence Assay
Purity
IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of Biotin under mild conditions to give a high degree of substitution.
Pricing
$540/1 mL | $2,275/5x1 mL

Serum, Animal Serum (Cat# AAA539340)

Full Name
Calf Serum (Sterile Filtered)
Pricing
$185/100 mL | $815/5x100 mL
Western Blot (WB) (Western blot All lanes: Casein kinase II subunit beta antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Gamma-aminobutyric acid receptor-associated protein, Polyclonal Antibody (Cat# AAA715566)

Full Name
Rabbit anti-human Gamma-aminobutyric acid receptor-associated protein polyclonal Antibody, Biotin conjugated
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg

Agarose Immobilized Goat anti-6-his, Antibody (Cat# AAA194005)

Full Name
6-His Affinity Purified Antibody, Agarose Immobilized
Applications
Immunoprecipitation
Pricing
$405/0.1 mg | $1,760/5x0.1 mg
Western Blot (WB) (Western blot All lanes: 60S ribosomal protein L11 antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 118kDa Additional bands at: 25,30,40?0 kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

60S ribosomal protein L11, Polyclonal Antibody (Cat# AAA715217)

Full Name
Rabbit anti-human 60S ribosomal protein L11 polyclonal Antibody, HRP conjugated
Gene Names
RPL11; L11; DBA7; GIG34
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Anti-LAMC2 antibody, MBS175527, Western blottingAll lanes: Anti LAMC2 (MBS175527) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: SW620 Whole Cell Lysate at 40ugPredicted bind size: 131KDObserved bind size: 131KD )

LAMC2, Polyclonal Antibody (Cat# AAA175527)

Full Name
Anti-LAMC2 Antibody
Gene Names
LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
Reactivity
Cross-Reactivity: No cross reactivity with other proteins
Reacts with: Human, Rat
Predict to work with: Mouse
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western Blot analysis of mouse brain tissue lysates (35ug/lane) using MBS6002753. This demonstrates that MBS6002753 detected the Irgm1 protein (arrow).)

Irgm1, Monoclonal Antibody (Cat# AAA6002753)

Full Name
Mouse anti-mouse Irgm1, CT
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Mouse
Applications
EL/EIA, WB
Purity
Purified by ammonium sulfate precipitation.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Immunohistochemistry (IHC) (Anti-Fibrinogen antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Fibrinogen, Polyclonal Antibody (Cat# AAA242115)

Full Name
Sheep Polyclonal (IgG) to Human Fibrinogen
Reactivity
Human
Applications
IHC - Paraffin, IHC - Frozen, IF, EIA
Purity
Protein G Purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL

Goat anti Human J chain of dimeric IgA, Polyclonal Secondary Antibody (Cat# AAA571594)

Full Name
Goat anti Human J chain of dimeric IgA, conjugated with Horseradish peroxidase
Reactivity
Human
Applications
EIA, IHC, paraffin, IHC-P, DB
Pricing
$660/1 mL | $2,810/5x1 mL

Complement C1, Active Protein (Cat# AAA537087)

Full Name
Complement C1 protein
Pricing
$610/0.2 mg | $2,550/5x0.2 mg

AKT1, Blocking Peptide (Cat# AAA542342)

Full Name
AKT1 Peptide
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Applications
Immmunodepletion, Competition Assay
Pricing
$280/0.25 mg | $1,110/5x0.25 mg

Dengue Virus NS1, Monoclonal Antibody (Cat# AAA313033)

Full Name
MAb to Dengue Virus NS1
Applications
EIA, IF
Purity
> 90% pure. Protein A Chromatography
Pricing
$870/1 mg | $3,760/5x1 mg
SDS-PAGE

Kappa (?) Light Chain, Secondary Antibody (Cat# AAA688333)

Full Name
Goat anti-Human Kappa (?) Light Chain - Affinity Pure, DyLight650 Conjugate, min x w/mouse serum
Reactivity
Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum immunoglobulins
heavy chains on all human immunoglobulins
lambda light chains on all human immunoglobulins mouse serum proteins
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Human Kappa Chain.
Pricing
$400/0.5 mg | $1,645/5x0.5 mg

Tumor Type M2 Pyruvate Kinase (M2PK), Polyclonal Antibody (Cat# AAA340499)

Full Name
Tumor Type M2 Pyruvate Kinase (M2PK)
Applications
EIA, WB, DB, IHC
Pricing
$2,680/0.5 mg | $690/10 mg | $11,900/5x0.5 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human fetus stained with Collagen II antibody using peroxidase-conjugate and AEC chromogen. Note staining of matrix in the cartilaginous plates.)

Collagen Type II, Monoclonal Antibody (Cat# AAA530216)

Full Name
Collagen Type II antibody
Gene Names
COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
Reactivity
Chicken, Human, Mouse, Salamander
Applications
IF, IHC, WB
Purity
Collagen Type II antibody was purified by Protein G chromatography.
Pricing
$930/0.5 mL | $4,130/5x0.5 mL
SDS-PAGE (SDS-PAGE analysis (12%) of 1 ul of recombinant LipL32. Purity is >95% as determined by gel electrophoresis.)

lipl32, Recombinant Protein (Cat# AAA505042)

Full Name
lipl32 recombinant
Applications
EIA
Pricing
$335/0.1 mg | $945/1 mg | $1,755/2 mg | $3,760/5 mg

Dengue Virus NS1, Monoclonal Antibody (Cat# AAA313088)

Full Name
MAb to Dengue Virus NS1
Applications
EIA, IF
Purity
> 90% pure. Protein A Chromatography
Pricing
$870/1 mg | $3,760/5x1 mg

Brain Tissue Lysate, Native Protein (Cat# AAA537313)

Full Name
Human Brain Tissue Lysate
Applications
WB
Purity
ELISA grade
Pricing
$380/0.15 mg | $1,710/5x0.15 mg

Goat anti Human IgG IgA IgM (Fc specific), Polyclonal Secondary Antibody (Cat# AAA571179)

Full Name
Goat anti Human IgG IgA IgM (Fc specific), conjugated with Horseradish peroxidase
Reactivity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum with other species has not been tested in detail.
Applications
paraffin
Purity
The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodi
Pricing
$505/1 mL | $2,125/5x1 mL
Western Blot (WB) ()

14-3-3 alpha/beta, Polyclonal Antibody (Cat# AAA5302129)

Full Name
14-3-3 alpha/beta antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Applications
EIA, IHC, WB
Purity
14-3-3 alpha/beta antibody was purified by Ammonium Sulfate precipitation
Pricing
$415/0.1 mg | $1,850/5x0.1 mg
Western Blot (WB) (Western blot of 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with anti-Bsd (lanes 1 - 3). Molecular weight (MW) standards show that Bsd is expressed as a 13kDa band in only the transfected cells.)

Blasticidin S Deaminase, Polyclonal Antibody (Cat# AAA472010)

Full Name
Blasticidin S Deaminase
Applications
WB, ICC
Purity
Affinity Purified
Pricing
$415/0.1 mL | $1,720/5x0.1 mL

Influenza A (virions) H1N1, Polyclonal Antibody (Cat# AAA315102)

Full Name
Goat anti Influenza A-Virions
Purity
Protein A chromatography followed by covalent coupling to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated molar HRP:Ig
Pricing
$580/1 mL | $2,470/5x1 mL

Dengue Virus NS1, Monoclonal Antibody (Cat# AAA313065)

Full Name
MAb to Dengue Virus NS1
Applications
EIA, IF, LF
Purity
> 90% pure. Protein A Chromatography
Pricing
$870/1 mg | $3,760/5x1 mg

Influenza A (virions) H3N2, Polyclonal Antibody (Cat# AAA315128)

Full Name
Goat anti Influenza A
Purity
Sodium sulfate precipitation and ion-exchange chromatography purified IgG fraction coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzym
Pricing
$580/1 mL | $2,470/5x1 mL

Goat anti Human IgG IgA IgM (Fc specific), Polyclonal Secondary Antibody (Cat# AAA571123)

Full Name
Goat anti Human IgG IgA IgM (Fc specific), conjugated with FITC
Reactivity
Human
Applications
EIA, IHC, IHC, In, IF
Purity
The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodi
Pricing
$505/1 mL | $2,125/5x1 mL

HIV-1 (purified virions), Polyclonal Antibody (Cat# AAA315213)

Full Name
Goat anti HIV-1
Purity
>95% pure. Sodium sulfate precipitation and ion-exchange chromatography
Pricing
$505/1 mL | $2,130/5x1 mL
Western Blot (WB) (The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Hexokinase 1 recombinant protein Lane 2.: Hexokinase 2 recombinant protein Lane 3.: Hexokinase 3 recombinant protein Lane 4.: Hexokinase 4 recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: K562 cell lysate The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Brain tissue lysate)

Hexokinase, Monoclonal Antibody (Cat# AAA200057)

Full Name
Hexokinase antibody
Gene Names
HK1; HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase
Reactivity
Human
Applications
EIA, WB, ICC, IF
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL

Haptoglobin, Protein (Cat# AAA173105)

Full Name
Haptoglobin, Bovine Serum
Gene Names
HPR; HP; A-259H10.2
Purity
> or = 90% (SDS-PAGE, bands at 10 and 40kDa indicative of alpha and beta subunits)

Dengue Virus NS1, Monoclonal Antibody (Cat# AAA313077)

Full Name
MAb to Dengue Virus NS1
Applications
EIA, LF, IF
Purity
> 90% pure. Protein A Chromatography
Pricing
$870/1 mg | $3,760/5x1 mg
Western Blot (WB) (Western blot All lanes: Tax1-binding protein 3 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13kDa Observed band size: 70 kDa Additional bands at: 36 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Tax1-binding protein 3, Polyclonal Antibody (Cat# AAA715901)

Full Name
Rabbit anti-human Tax1-binding protein 3 polyclonal Antibody, FITC
Gene Names
TAX1BP3; TIP-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
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