Agarose Immobilized Goat anti-6-his Antibody
6-His Affinity Purified Antibody, Agarose Immobilized
Applications
Immunoprecipitation
Synonyms
Agarose Immobilized Goat anti-6-his; 6-His Affinity Purified Antibody; Agarose Immobilized; anti-Agarose Immobilized Goat anti-6-his antibody
Host
Goat
Specificity
Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Form/Format
0.1mg/0.4ml 50% gel slurry in Phosphate-Buffered Saline (PBS), pH 7.2, 0.09% sodium azide. Antibody coupled to agarose beads using cyanogen bromide.
Concentration
100 ug antibody / 200 ul gel (varies by lot)
Applicable Applications for anti-Agarose Immobilized Goat anti-6-his antibody
Immunoprecipitation
Application Notes
Immunoprecipitation: 15 to 25 ul of gell slurry per 0.1 to 1 mg of protein lysate or extract.
Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system.
Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system.
Antigen
6-His (HHHHHH) coupled to KLH. Antibody coupled to agarose beads using a cyanogen bromide method.
Buffer
0.01M Phosphate, pH 7.2, 0.1M NaCl
Stabilizer
None
Preservatives
0.1% sodium azide
Preparation and Storage
This antibody is stable for at least one year at 2-8 degree C.
Related Product Information for anti-Agarose Immobilized Goat anti-6-his antibody
A polystidine-tag consists of at least six histidine (His) residues, often at the N-or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Product Categories/Family for anti-Agarose Immobilized Goat anti-6-his antibody
Similar Products
Product Notes
The Agarose Immobilized Goat anti-6-his (Catalog #AAA194005) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Agarose Immobilized Goat anti-6-his can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation. Immunoprecipitation: 15 to 25 ul of gell slurry per 0.1 to 1 mg of protein lysate or extract. Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system. Researchers should empirically determine the suitability of the Agarose Immobilized Goat anti-6-his for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Agarose Immobilized Goat anti-6-his, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
