Rabbit PHAP (NT) Polyclonal Antibody | anti-phaP antibody
PHAP (NT) Polyclonal Antibody
Applications
Western Blot
Synonyms
PHAP (NT); Polyclonal Antibody; PHAP (NT) Polyclonal Antibody; anti-phaP antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
PHAP (NT) [Rabbit polyclonal antibody to PHAP (NT)]
This antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins (tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.
This antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins (tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.
Form/Format
100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Sequence Length
174
Applicable Applications for anti-phaP antibody
Western Blot
Application Notes
Western Blot: Use at 0.5-2 ug/ml. Using Raji cell lysate as a positive control, a band of approximately 35 kD is detected corresponding to PHAP III, with a doublet below PHAP III corresponding to PHAP I (upper) and PHAP I2a (lower).
Antigen
A synthetic peptide close to the amino terminus of human PHAP.
Stabilizer
None
Preservatives
0.02% sodium azide
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
Store at 4 degree C; stable for one year
Product Categories/Family for anti-phaP antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PHAP (NT) phap (Catalog #AAA194326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PHAP (NT) can be used in a range of immunoassay formats including, but not limited to, Western Blot. Western Blot: Use at 0.5-2 ug/ml. Using Raji cell lysate as a positive control, a band of approximately 35 kD is detected corresponding to PHAP III, with a doublet below PHAP III corresponding to PHAP I (upper) and PHAP I2a (lower). Researchers should empirically determine the suitability of the PHAP (NT) phap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHAP (NT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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