Sheep anti-Human Fibrinogen Polyclonal Antibody | anti-FGG antibody

Sheep Polyclonal (IgG) to Human Fibrinogen

Cat. Num. AAA242115

• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Protein G Purified
• Synonyms: Fibrinogen; Polyclonal Antibody; Sheep Polyclonal (IgG) to Human Fibrinogen; Anti-Fibrinogen Antibody IHC-plus; Human Fibrinogen; anti-FGG antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human fibrinogen, a complex ~340kD hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of alpha, beta and gamma chains linked by a series of 29 disulphide bonds. The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the alpha chains during fibrin clot cross-linking. Shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse. Has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4, 0.09% sodium azide.
• Concentration: 5 mg/ml (varies by lot)
• Sequence Length: 444

• Applicable Applications for anti-FGG antibody: Immunohistochemistry (IHC - Paraffin, IHC - Frozen), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: ELISA (1:50 - 1:100), IF, IHC-Fr, IHC-P (5 ug/ml)
• Target: Fibrinogen
• Immunogen Description: Human Fibrinogen purified from plasma.
• Immunogen Type: Purified protein
• Immunogen: Fibrinogen antibody was raised against human Fibrinogen purified from plasma.
• Usage: Immunohistochemistry: MBS242115 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242115 was determined to be 5 ug/ml.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles.; Microcentrifugation recommended if solution contains precipitate.

Immunohistochemistry (IHC)

(Anti-Fibrinogen antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 350
• NCBI GeneID: 280792
• UniProt Accession #: P12799
• NCBI Official Full Name: fibrinogen
• NCBI Official Synonym Full Names: fibrinogen gamma chain
• NCBI Official Symbol: FGG
• NCBI Protein Information: fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide
• UniProt Protein Name: Fibrinogen gamma-B chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGG
• UniProt Synonym Gene Names: Gamma'
• UniProt Entry Name: FIBG_BOVIN

Uniprot Description

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.