Western Blot (WB)
(Fig.1. WB experiment of beta-Actin expression, the samples are Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3) each 10 ug, the primary antibody is beta-Actin mouse monoclonal antibody ( 1C7) (1:5000), the secondary antibody is goat anti-mouse IgG (1: 10000). )
Universal Loading Control Antibody Cocktail Loading Control Kit
Universal Loading Control Antibody Cocktail
Applications
Western Blot
Synonyms
Universal Loading Control Antibody Cocktail; Universal Loading Control Antibody Cocktail loading control kit
Applicable Applications for Universal Loading Control Antibody Cocktail loading control kit
Western Blot (WB)
Tag
Loading Control
Features & Benefits
The universal internal reference antibody set is based on the innovation and optimization of the classic WB combination kit, which increases the storage time of the diluted antibody and can be used repeatedly.
Universal Loading Control Antibody Cocktail provides a comprehensive and detailed method to quickly and conveniently detect the content of load control proteins in a variety of samples.
Universal Loading Control Antibody Cocktail provides a comprehensive and detailed method to quickly and conveniently detect the content of load control proteins in a variety of samples.
Kit Components
Anti-beta-Actin Mouse Monoclonal Antibody (1C7)
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6)
SuperKine Enhanced Antibody Diluention Buffer
WB Positive Control
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6)
SuperKine Enhanced Antibody Diluention Buffer
WB Positive Control
Usage Notes
Do not mix components from different Lot and manufacturers; Otherwise, the result may be abnormal.
To obtain the best experimental results, you need to optimize all of your experimental elements, including sample number, antibody concentration, membrane and sealant use.
Please select appropriate and stable reference antibodies according to the sample and target protein.As in adipose tissue, beta-tubulin may be unstable.
Please follow the detailed instructions of each component.
To obtain the best experimental results, you need to optimize all of your experimental elements, including sample number, antibody concentration, membrane and sealant use.
Please select appropriate and stable reference antibodies according to the sample and target protein.As in adipose tissue, beta-tubulin may be unstable.
Please follow the detailed instructions of each component.
Preparation and Storage
Store according to the recommended storage conditions of each component, stable for 12 months from date of shipment.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Western Blot (WB)
(Fig.1. WB experiment of beta-Actin expression, the samples are Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3) each 10 ug, the primary antibody is beta-Actin mouse monoclonal antibody ( 1C7) (1:5000), the secondary antibody is goat anti-mouse IgG (1: 10000). )
Western Blot (WB)
(Fig.1. WB experiment of beta-Actin expression, the samples are Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3) each 10 ug, the primary antibody is beta-Actin mouse monoclonal antibody ( 1C7) (1:5000), the secondary antibody is goat anti-mouse IgG (1: 10000). )
Western Blot (WB)
(Fig.2. WB experiment of GAPDH, the samples are Hela (lane 1), rat brain (lane 2), Mouse brain (lane 5) each 10 ug, the primary antibody is GAPDH mouse monoclonal antibody (2B5) (1: 10000), the secondary antibody is goat anti-mouse IgG (1: 10000). )
Western Blot (WB)
(Fig.2. WB experiment of GAPDH, the samples are Hela (lane 1), rat brain (lane 2), Mouse brain (lane 5) each 10 ug, the primary antibody is GAPDH mouse monoclonal antibody (2B5) (1: 10000), the secondary antibody is goat anti-mouse IgG (1: 10000). )
Western Blot (WB)
(Fig.3. WB experiment of beta-Tubulin, the samples are Rat brain (lane 1), A549 cell (lane 2), Mouse brain (lane 3) each 10 ug, the primary antibody is beta-Tubulin mouse monoclonal antibody (2B5) (1:10000), the secondary antibody is goat anti-mouse IgG (1:10000).)
Western Blot (WB)
(Fig.3. WB experiment of beta-Tubulin, the samples are Rat brain (lane 1), A549 cell (lane 2), Mouse brain (lane 3) each 10 ug, the primary antibody is beta-Tubulin mouse monoclonal antibody (2B5) (1:10000), the secondary antibody is goat anti-mouse IgG (1:10000).)
Related Product Information for Universal Loading Control Antibody Cocktail loading control kit
Description: Loading Control
Background: The Universal Internal Reference Antibody Cocktail is a set containing the antibodies beta-actin, GAPDH and beta-tubulin commonly used in immunoassay (WB, IHC-P, IF) as well as the antibody diluent.There are many kinds of internal reference antibodies. Therefore, the following three principles should be followed in choosing an appropriate internal reference antibody: 1.Considering the sample species, for mammalian tissues and cells, the commonly used reference proteins are beta-actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+-ATPase, etc.For Plant samples, Plant Actin and RuBisCo are commonly used.Consider the molecular weight of the target protein. 2. The selected reference protein should have a different molecular weight from the target protein (>5kDa) to distinguish the target protein bands. For high molecular weight proteins (over 100kDa), we recommend that our clients use Vinculin as an internal reference; 2. Considering the expression and localization of target protein, select the corresponding internal reference, such as whole cell, nucleus, mitochondria, cell membrane and so on.
Background: The Universal Internal Reference Antibody Cocktail is a set containing the antibodies beta-actin, GAPDH and beta-tubulin commonly used in immunoassay (WB, IHC-P, IF) as well as the antibody diluent.There are many kinds of internal reference antibodies. Therefore, the following three principles should be followed in choosing an appropriate internal reference antibody: 1.Considering the sample species, for mammalian tissues and cells, the commonly used reference proteins are beta-actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+-ATPase, etc.For Plant samples, Plant Actin and RuBisCo are commonly used.Consider the molecular weight of the target protein. 2. The selected reference protein should have a different molecular weight from the target protein (>5kDa) to distinguish the target protein bands. For high molecular weight proteins (over 100kDa), we recommend that our clients use Vinculin as an internal reference; 2. Considering the expression and localization of target protein, select the corresponding internal reference, such as whole cell, nucleus, mitochondria, cell membrane and so on.
Product Categories/Family for Universal Loading Control Antibody Cocktail loading control kit
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Product Notes
The Universal Loading Control Antibody Cocktail (Catalog #AAA9719226) is a Loading Control Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Universal Loading Control Antibody Cocktail can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Universal Loading Control Antibody Cocktail for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Universal Loading Control Antibody Cocktail, Loading Control Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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