Western Blot (WB)
(Western blot V-type proton ATPase subunit G 1 antibody at at 2 /ml+ 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rabbit V-type proton ATPase subunit G 1 Polyclonal Antibody | anti-ATP6V1G1 antibody
Rabbit anti-human V-type proton ATPase subunit G 1 polyclonal Antibody, FITC
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(Western blot V-type proton ATPase subunit G 1 antibody at at 2 /ml+ 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Western Blot (WB)
(Western blot V-type proton ATPase subunit G 1 antibody at at 2 /ml+ 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
ATP6V1G1: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase G subunit family.
Protein type: Hydrolase; Vesicle
Chromosomal Location of Human Ortholog: 9q32
Cellular Component: cytosol; lysosomal membrane; plasma membrane; vacuolar proton-transporting V-type ATPase complex
Molecular Function: ATPase binding; hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding
Biological Process: cell redox homeostasis; cellular iron ion homeostasis; insulin receptor signaling pathway; metabolic process; transferrin transport; transmembrane transport
Research Articles on ATP6V1G1
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Product Notes
The ATP6V1G1 atp6v1g1 (Catalog #AAA715298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human V-type proton ATPase subunit G 1 polyclonal Antibody, FITC reacts with Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's V-type proton ATPase subunit G 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the ATP6V1G1 atp6v1g1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V-type proton ATPase subunit G 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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