CASPASE-10 Assay Kit | CASP10 assay kit

FAM FLICA CASPASE-10 KIT

Cat. Num. AAA239207

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CASPASE-10; FAM FLICA CASPASE-10 KIT; CASP10 assay kit

• Form/Format: Kit
• Sequence Length: 521

• Applicable Applications for CASP10 assay kit: Flow cytometry (FC/FACS), Immunofluorescence (IF)
• Reagents In The Kits: 4 vials of FAM-AEVD-FMK FLICA Reagent - lyophilized ; 10X Wash Buffer, 60 mL ; Fixative, 6 mL ; Propidium Iodide, 1 mL ; Hoechst Stain, 1 mL
• Preparation and Storage: Store the unopened kit (and each unopened component) at 4 degree C until the expiration date. ; Protect the FLICA reagent from light at all times. ; Once reconstituted, the 150X FLICA stock should be stored at -20 degree C protected from light.; Shelf Life: The 150X FLICA reconstituted stock is stable for up to 6 months and may be thawed twice during that time.

Testing Data #1

(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)

Related Product Information for CASP10 assay kit

Description: The FAM FLICA Caspase 10 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
Test Principle: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

NCBI and Uniprot Product Information

• NCBI GI #: 47078269
• NCBI GeneID: 843
• NCBI Accession #: NP_116756.2
• NCBI GenBank Nucleotide #: NM_032974.4
• Molecular Weight: 28,364 Da
• NCBI Official Full Name: caspase-10 isoform 2 preproprotein
• NCBI Official Synonym Full Names: caspase 10, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10; CASP-10; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; ICE-like apoptotic protease 4; apoptotic protease MCH-4; caspase 10, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2
• UniProt Entry Name: CASPA_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.63

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; plasma membrane; CD95 death-inducing signaling complex; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding

Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; innate immune response; proteolysis

Disease: Lymphoma, Non-hodgkin, Familial; Gastric Cancer; Autoimmune Lymphoproliferative Syndrome, Type Iia