Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): A549 and 231 cell Amount of lysate: 40ug per lane Primary antibody: 1/1000 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Rabbit anti-Human NEK11 Polyclonal Antibody | anti-NEK11 antibody
NEK11 Antibody
Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): A549 and 231 cell Amount of lysate: 40ug per lane Primary antibody: 1/1000 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): A549 and 231 cell Amount of lysate: 40ug per lane Primary antibody: 1/1000 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]
Uniprot Description
NEK11: Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation. Interacts with isoform 1 of NEK2. Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. Autorepressed by intramolecular binding of the C-terminus which dissociates following phosphorylation by NEK2 isoform 1 in G1/S-arrested cells. NEK2 isoform 2 is largely not present in the nucleolus, and does not appear to phosphorylate NEK11. Activated in response to DNA damage. Inhibited by zinc. Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Nucleolus; Kinase, protein; EC 2.7.11.1; Other group; NEK family
Chromosomal Location of Human Ortholog: 3q22.1
Cellular Component: nucleoplasm; nucleolus
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding
Biological Process: intra-S DNA damage checkpoint; G2/M transition DNA damage checkpoint; protein amino acid phosphorylation
Research Articles on NEK11
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Product Notes
The NEK11 nek11 (Catalog #AAA9402069) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK11 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500-1:2000. Researchers should empirically determine the suitability of the NEK11 nek11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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