Rabbit TNFAIP1 Polyclonal Antibody | anti-TNFAIP1 antibody

TNFAIP1 Antibody

Cat. Num. AAA154370

• Gene Names: TNFAIP1; B12; B61; EDP1; BTBD34; hBACURD2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: TNFAIP1; Polyclonal Antibody; TNFAIP1 Antibody; Tumor necrosis factor; alpha-induced protein 1; BTB/POZ domain-containing protein TNFAIP1; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BACURD2; EDP1; anti-TNFAIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TNFAIP1 antibody is human, mouse and rat reactive. TNFAIP1 antibody is predicted to not cross-react with other TNFAIP proteins.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 316

• Applicable Applications for anti-TNFAIP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: TNFAIP1 antibody can be used for the detection of TNFAIP1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry at 10 mug/ml.
• Immunogen: TNFAIP1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TNFAIP1.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: Mouse Brain Tissue Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 10 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 20 μg/ml.Red: TNFAIP1 Antibody (8345)Blue: DAPI staining)

Related Product Information for anti-TNFAIP1 antibody

TNFAIP1 is a BTB/POZ domain-containing protein that belongs to the KCTD10/BACURD family which mediates POZ-POZ interactions and chromatin modeling (1,2). The expression of TNFAIP1 is developmentally regulated in a tissue-specific manner and can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells (3). TNFAIP1 may be involved in DNA repair, DNA synthesis, and cell apoptosis. TNFAIP1 is suggested to play a role in the process of cancer and in the innate immunity against the Hepatitis B virus (4,5).

Product Categories/Family for anti-TNFAIP1 antibody

Apoptosis; Cancer; Innate Immunity

References

Liu M, Sun Z, Zhou A, et al. Functional characterization of the promoter region of human TNFAIP1 gene. Mol. Biol. Rep. 2010; 37:1699-705. Hu X, Yan F, Wang F, et al. TNFAIP1 interacts with KCTD10 to promote the degradation of KCTD10 proteins and inhibit the transcriptional activities of NF-kappaB and AP-1. Mol. Biol. Rep. 2012; 39:9911-9. Zhang CL, Wang C, Yan WJ, et al. Knockdown of TNFAIP1 inhibits growth and induces apoptosis in osteosarcoma cells through inhibition of the nuclear factor-?B pathway. Oncol. Rep. 2014; 32:1149-55. Kim DM, Chung KS, Choi SJ, et al. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer 2009; 125:2520-7.

NCBI and Uniprot Product Information

• NCBI GI #: 10863937
• NCBI GeneID: 7126
• NCBI Accession #: NP_066960
• NCBI GenBank Nucleotide #: NM_021137.4
• UniProt Accession #: Q13829; Q5TZQ1; B7Z6M4
• Molecular Weight: Predicted: 35 kDa; Observed: 35 kDa
• NCBI Official Full Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• NCBI Official Synonym Full Names: TNF alpha induced protein 1
• NCBI Official Symbol: TNFAIP1
• NCBI Official Synonym Symbols: B12; B61; EDP1; BTBD34; hBACURD2
• NCBI Protein Information: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• UniProt Protein Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• Protein Family: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein
• UniProt Gene Name: TNFAIP1
• UniProt Synonym Gene Names: BACURD2; EDP1; hBACURD2
• UniProt Entry Name: BACD2_HUMAN

NCBI Description

This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFAIP1: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity. Belongs to the BACURD family.

Protein type: Ubiquitin conjugating system; DNA replication

Chromosomal Location of Human Ortholog: 17q22-q23

Cellular Component: cytoplasm; endosome; nucleolus

Molecular Function: GTP-Rho binding; protein binding; protein domain specific binding; ubiquitin-protein ligase activity

Biological Process: apoptosis; cell migration; DNA replication; embryonic development; immune response; negative regulation of Rho protein signal transduction; positive regulation of DNA replication; proteasomal ubiquitin-dependent protein catabolic process; protein homooligomerization; protein ubiquitination; stress fiber formation