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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
649 results for " ROS" - showing 1-50
Typical Testing Data/Standard Curve (for reference only)
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate), Assay Kit (Cat# AAA168933)
Full Name
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric)
Pricing
$520/100 Assays | $1,905/5x100 Assays
Testing Data
OxiSelect In Vitro ROS/RNS, Assay Kit (Cat# AAA168257)
Full Name
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
Pricing
$395/20 Assays | $690/96 Assays | $2,630/5x96 Assays
Typical Testing Data/Standard Curve (for reference only)
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate), Assay Kit (Cat# AAA168898)
Full Name
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Fluorometric)
Pricing
$570/100 Assays | $2,010/5x100 Assays
Typical Testing Data/Standard Curve (for reference only)
OxiSelect Intracellular ROS, Assay Kit (Cat# AAA168048)
Full Name
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
Pricing
$405/20 Assays | $715/96 Assays | $2,730/5x96 Assays
Testing Data
OxiSelect Intracellular Nitric Oxide (NO), Assay Kit (Cat# AAA168222)
Full Name
OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)
Pricing
$565/96 Assays | $2,100/5x96 Assays
Testing Data
OxiSelect Myeloperoxidase Activity, Assay Kit (Cat# AAA168030)
Full Name
OxiSelect Myeloperoxidase Activity Assay Kit (Colorimetric)
Pricing
$635/200 Assays | $2,905/5x200 Assays
guanylate cyclase 2D, membrane (retina-specific), ELISA Kit (Cat# AAA9331774)
Full Name
Human Retinal guanylyl cyclase 1, GUCY2D ELISA Kit
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of ROS (pY2114) expression in SKOVCAR (A), Raw264.7 (B), PC12 (C) whole cell lysates.)
ROS (pY2114), Polyclonal Antibody (Cat# AAA821029)
Full Name
Anti-ROS (pY2114) Phospho Antibody
Gene Names
ROS1; ROS; MCF3; c-ros-1
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Pig, Rabbit
Applications
WB
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$185/0.03 mL | $275/0.1 mL | $380/0.2 mL | $1,650/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)
Reactive oxygen species modulator 1, ELISA Kit (Cat# AAA2882091)
Full Name
Human Reactive oxygen species modulator 1 ELISA Kit
Gene Names
ROMO1; MTGM; MTGMP; C20orf52; bA353C18.2
Reactivity
Human
Pricing
$380/48-Strip-Wells | $510/96-Strip-Wells | $2,210/5x96-Strip-Wells | $4,070/10x96-Strip-Wells
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9205943 cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Cleaved LC3B, Polyclonal Antibody (Cat# AAA9205943)
Full Name
Cleaved LC3B Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
ROMO1, ELISA Kit (Cat# AAA2515649)
Full Name
Human ROMO1(Reactive oxygen species modulator 1)ELISA Kit
Gene Names
ROMO1; MTGM; MTGMP; C20orf52; bA353C18.2
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Activity Data
(PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.)
Parathyroid Hormone (PTH), Active Protein (Cat# AAA2097252)
Full Name
Active Parathyroid Hormone (PTH)
Gene Names
Pth; Pth1; Pthr1; PTH-(1-84)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Western Blot (WB)
(Western Blot analysis of various tissues using Mouse anti Sheep CD230, clone ROS-BC6 . Lane 1 PRPc BSE infected ovine medulla, Lane 2 PRPc uninfected ovine medulla, Lane 3 Scrapie infected ovine medulla, Lane 4 PRPsc scrapie infected ovine spleen, Lane 5 PRPsc BSE infected ovine medulla, Lane 6 PRPsc BSE infected ovine spleen. Image courtesy of The Roslin Institute)
CD230, Monoclonal Antibody (Cat# AAA225678)
Full Name
Mouse Anti Sheep CD230 (aa144-154)
Gene Names
PRNP; Prp; SIP; PRPC
Reactivity
Sheep, Bovine, Hamster, Mouse, Red Deer
Does not react with: Human
Does not react with: Human
Applications
FC/FACS, IHC, WB
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Testing Data
(Flow Cytometry analysis of ovine PBMC's using Mouse anti Sheep CD230, clone ROS-BH1 . Images courtesy of The Roslin Institute)
CD230, Monoclonal Antibody (Cat# AAA225677)
Full Name
Mouse Anti Sheep CD230 (aa143-154)
Gene Names
PRNP; Prp; SIP; PRPC
Reactivity
Sheep, Bovine, Human, Hamster, Mouse, Red Deer
Applications
FC/FACS, IHC, WB
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Testing Data
(Mouse anti Chicken CSF1R (CD115) immunofluorescence staining of chicken bursa of Fabricius. Cryostat sections of 6 week old chicken bursa of Fabricius. A) Clone ROS-AV170 identifies strong membrane-bound immunoreaction on the cell surface of the bursal secretory dendritic cells (BSDC). B) Double immunofluorescence staining with Mouse anti vimentin, clone 3B4 (green), (product code OBT3104) and Mouse anti Chicken CSF1R, clone ROS-AV170 (red), (product code This product) shows that colocalization of vimentin with CSF1R occurs only in bursal dendritic cells. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary)
CSF1R, Monoclonal Antibody (Cat# AAA224833)
Full Name
MOUSE ANTI CHICKEN CSF1R
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Chicken
Applications
IHC, FC/FACS, IF
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of Rat brain tissue sample, using ROS Antibody(MBS9603202).)
ROS, Polyclonal Antibody (Cat# AAA9603202)
Full Name
ROS Antibody
Gene Names
ROS1; ROS; MCF3; c-ros-1
Reactivity
Human, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant ROS1, Human.)
C-Ros Oncogene 1, Polyclonal Antibody (Cat# AAA2027852)
Full Name
Polyclonal Antibody to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1)
Gene Names
ROS1; ROS; MCF3; c-ros-1
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
SDS-Page
(Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-6: Mouse heart tissue, Human heart tissue, HEPG2 cell, PC-3 cell, 231 cell and 293T cell lysates, Primary antibody: MBS9430580 (ROMO1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
ROMO1, Polyclonal Antibody (Cat# AAA9430580)
Full Name
ROMO1 Antibody
Gene Names
ROMO1; MTGM; MTGMP; C20orf52; bA353C18.2
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Antigen affinity purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Immunofluorescence (IF)
(MBS9604601 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
APEX1, Polyclonal Antibody (Cat# AAA9604601)
Full Name
APEX1 Antibody
Gene Names
APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215797)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$375/0.1 mg | $1,535/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of Hela, using APEX1 antibody. The lane on the left is treated with the antigen-specific peptide.)
APEX1, Polyclonal Antibody (Cat# AAA9606912)
Full Name
APEX1 Antibody
Gene Names
APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Anti-CEACAM Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CEACAM, Polyclonal Antibody (Cat# AAA9204586)
Full Name
CEACAM Antibody (N-term)
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Flow Cytometry (FC/FACS)
(Staining of dog bone marrow derived macrophages (BMDMs) using Mouse anti pig CSF1-R, clone ROS 8G11 . Image courtesy of the Roslin Institute)
CSF1R, Monoclonal Antibody (Cat# AAA224795)
Full Name
MOUSE ANTI PIG CSF1R: FITC
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Pig
Applications
FC/FACS
Pricing
$560/0.1 mg | $2,365/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
LC3, Polyclonal Antibody (Cat# AAA9210748)
Full Name
LC3 Antibody (APG8A/B)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human
Predicted: Zebrafish, Bovine,Mouse, Rat
Predicted: Zebrafish, Bovine,Mouse, Rat
Applications
WB, EIA, IHC
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Peroxiredoxin 4 Monoclonal Antibody (CPTC-PRDX4-2).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Peroxiredoxin 4, Monoclonal Antibody (Cat# AAA4380528)
Full Name
Peroxiredoxin 4 (Prognostic Marker for Lung SqCC)
Gene Names
PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n
Reactivity
Human. Others not tested.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF)
PGCG, Polyclonal Antibody (Cat# AAA540095)
Full Name
PGCG Antibody
Reactivity
Human, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, WB
Pricing
$490/0.1 mg | $2,065/5x0.1 mg
Flow Cytometry (FC/FACS)
(Staining of dog bone marrow derived macrophages (BMDMs) using Mouse anti pig CSF1-R, clone ROS 8G11 . Image courtesy of the Roslin Institute)
CSF1R, Monoclonal Antibody (Cat# AAA224825)
Full Name
MOUSE ANTI PIG CSF1R: APC
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Pig
Applications
FC/FACS
Pricing
$630/100 Tests | $2,680/5x100 Tests
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215791)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Testing Data
(Flow cytometry analysis of CD230. Ovine PBMC's staining using Mouse anti Sheep CD230, clone ROS-1H9 as the primary antibody followed by anti mouse IgG conjugated to APC. Gated on sytox blue negative live cells and the PBMC region determined by FSC v. SSC profile. Image courtesy of The Roslin Institute.)
CD230, Monoclonal Antibody (Cat# AAA225679)
Full Name
Mouse Anti Sheep CD230 (aa140-aa145)
Gene Names
PRNP; Prp; SIP; PRPC
Reactivity
Sheep, Bovine, Human, Hamster, Mouse, Red Deer
Applications
FC/FACS, IHC, WB
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-ROS1 Antibody (C-term) at 1:1000 dilutionLane 1: U-87 MG whole cell lysatesLane 2: NCI-H1299 whole cell lysatesLane 3: Karpas-299 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 264 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ROS1, Polyclonal Antibody (Cat# AAA9232057)
Full Name
ROS1 Antibody (C-term)
Gene Names
ROS1; ROS; MCF3; c-ros-1
Reactivity
Human
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215792)
Full Name
RAT ANTI MOUSE DECTIN-1:Low Endotoxin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, FN, IP
Pricing
$830/0.5 mg | $3,580/5x0.5 mg
Immunohistochemistry (IHC)
(MBS9603451 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PRPH2, Polyclonal Antibody (Cat# AAA9603451)
Full Name
PRPH2 Antibody
Gene Names
PRPH2; DS; RDS; RP7; rd2; AVMD; PRPH; AOFMD; CACD2; MDBS1; TSPAN22
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215795)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$695/0.5 mg | $2,975/5x0.5 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GUCY2D antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
GUCY2D, Polyclonal Antibody (Cat# AAA128992)
Full Name
GUCY2D Polyclonal Antibody
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Rhodopsin / RHO, Monoclonal Antibody (Cat# AAA249482)
Full Name
Anti-Rhodopsin / RHO Antibody (clone B630) IHC-plus
Gene Names
RHO; RP4; OPN2; CSNBAD1
Reactivity
Mouse, Bovine, Rat, Pig, Human
Applications
IHC, ICC, IF, WB
Purity
Protein G purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Western Blot (WB)
(Western Blot using anti-Rhodopsin antibody Rho 1D4 Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of Rho 1D4 at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat (Rattus norvegicus) Rhodopsin is 39.0kDa, though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al., PMID: 16492772, PMID: 10798675). successfully detected monomeric, dimeric and multimeric Rhodopsin in rat eye lysate.)
Rhodopsin, Monoclonal Recombinant Antibody (Cat# AAA488416)
Full Name
Anti-Rhodopsin [Rho 1D4]
Gene Names
RHO; RP4; OPN2; CSNBAD1
Reactivity
Mouse, Rat, Cow, Human, Zebrafish, Amphibians
Applications
WB, EIA, IP, IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215800)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$210/0.025 mg | $800/5x0.025 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215789)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$525/0.25 mg | $2,210/5x0.25 mg
Western Blot (WB)
PGCD, Polyclonal Antibody (Cat# AAA540092)
Full Name
PGCD Antibody
Reactivity
Human, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215798)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215793)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of mouse eye, using GUCY2F antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
GUCY2F, Polyclonal Antibody (Cat# AAA9134248)
Full Name
GUCY2F Polyclonal Antibody
Gene Names
GUCY2F; CYGF; GC-F; GUC2F; GUC2DL; RETGC-2; ROS-GC2
Reactivity
Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215799)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215790)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215796)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215794)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$280/0.05 mg | $1,115/5x0.05 mg
Western Blot (WB)
(GUCY2D Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the GUCY2D antibody detected the GUCY2D protein (arrow).)
GUCY2D, Polyclonal Antibody (Cat# AAA9206384)
Full Name
GUCY2D Antibody (Center)
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western Blot using anti-Rhodopsin antibody Rho 1D4 Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of Rho 1D4 (MBS488415) at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat (Rattus norvegicus) Rhodopsin is 39.0kDa, though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al., PMID: 16492772, PMID: 10798675). MBS488415 successfully detected monomeric, dimeric and multimeric Rhodopsin in rat eye lysate.)
Rhodopsin, Monoclonal Recombinant Antibody (Cat# AAA488415)
Full Name
Anti-Rhodopsin [Rho 1D4]
Gene Names
RHO; RP4; OPN2; CSNBAD1
Reactivity
Mouse, Rat, Cow, Human, Zebrafish, Amphibians
Applications
WB, EIA, IP, IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Reactive Oxygen Species (ROS) Detection, Assay Kit (Cat# AAA9719224)
Full Name
Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit
Pricing
$205/100 Tests | $175/50 Tests | $935/5x100 Tests
Immunoflurescence (IF)/Immunocytochemistry (ICC)
(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(BF8013) and rabbit anti-beta tubulin Ab(AF7011) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
p53, Monoclonal Antibody (Cat# AAA9629032)
Full Name
p53 Mouse monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse
Applications
WB, IF, ICC
Purity
Affinity-chromatography.
Pricing
$280/0.1 mL | $325/0.2 mL | $1,305/5x0.2 mL
