Mouse CD230 Monoclonal Antibody | anti-CD230 antibody

Mouse Anti Sheep CD230 (aa144-154)

Cat. Num. AAA225678

• Gene Names: PRNP; Prp; SIP; PRPC
• Reactivity: Sheep, Bovine, Hamster, Mouse, Red Deer; Does not react with: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: CD230; Monoclonal Antibody; Mouse Anti Sheep CD230 (aa144-154); CD230 antibody (ROS-BC6); anti-CD230 antibody

• Host: Mouse
• Reactivity: Sheep, Bovine, Hamster, Mouse, Red Deer; Does not react with: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ROS-BC6
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Liquid; Phosphate Buffered Saline
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 256

• Applicable Applications for anti-CD230 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Immunogen: A truncated form of recombinant Ovine PRP spanning residues aa94-aa233.
• Fusion Partners: Spleen cells from immunised 129/Ola mice were fused with cells of the SP2/0 myeloma cell line.
• Preservative Stabilizers: 0.09% Sodium Azide (NaN3)
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.

Western Blot (WB)

(Western Blot analysis of various tissues using Mouse anti Sheep CD230, clone ROS-BC6 . Lane 1 PRPc BSE infected ovine medulla, Lane 2 PRPc uninfected ovine medulla, Lane 3 Scrapie infected ovine medulla, Lane 4 PRPsc scrapie infected ovine spleen, Lane 5 PRPsc BSE infected ovine medulla, Lane 6 PRPsc BSE infected ovine spleen. Image courtesy of The Roslin Institute)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of CD230. Ovine PBMC's staining using Mouse anti Sheep CD230, clone ROS-BC6 as the primary antibody followed by an anti mouse IgG conjugated to APC. Gated on sytox blue negative live cells and the PBMC region determined by FSC v. SSC profile. Image courtesy of The Roslin Institute.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of ovine PBMC's using Mouse anti Sheep CD230, clone ROS-BC6 . Images courtesy of the Roslin Institute)

Related Product Information for anti-CD230 antibody

Mouse anti Sheep CD230 (Prpsc) antibody, clone ROS-BC6 recognizes the prion disease form of prion protein known as CD230. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals and known as Scrapie (sheep and goats), BSE (cattle), CWD (cervine/deer) and CJD (humans). These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioral changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques.
CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties.

NCBI and Uniprot Product Information

• NCBI GI #: 57164159
• NCBI GeneID: 493887
• NCBI Accession #: NP_001009481.1
• NCBI GenBank Nucleotide #: NM_001009481.1
• UniProt Accession #: P23907; P10279
• NCBI Official Full Name: major prion protein
• NCBI Official Symbol: PRNP
• NCBI Official Synonym Symbols: Prp; SIP; PRPC
• NCBI Protein Information: major prion protein
• UniProt Protein Name: Major prion protein
• UniProt Gene Name: PRNP
• UniProt Synonym Gene Names: PRP; SIP; PrP
• UniProt Entry Name: PRIO_SHEEP

Uniprot Description

Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains ().