Rabbit LC3 Polyclonal Antibody | anti-MAP1LC3B antibody

LC3 Antibody (APG8A/B)

Cat. Num. AAA9210748

• Gene Names: MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• Reactivity: Human; Predicted: Zebrafish, Bovine,Mouse, Rat
• Applications: Western Blot, ELISA, Immunohistochemistry
• Synonyms: LC3; Polyclonal Antibody; LC3 Antibody (APG8A/B); Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; anti-MAP1LC3B antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Zebrafish, Bovine,Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from human LC3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification

• Applicable Applications for anti-MAP1LC3B antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:500; IHC-P~~1:50~100
• Function: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles(autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed: 28017329). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamilyis essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with there ticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomesupon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed: 31006538, PubMed: 31006537).
• Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid- anchor.Note=LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy(PubMed:28017329). Localizes also todiscrete punctae along the ciliary axoneme.
• Tissue Location: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine ,100ng/ml, using APG8a/b Antibody (Y38)(MBS9210748)(upper) orBeta-actin (lower).)

Related Product Information for anti-MAP1LC3B antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a and MAP1LC3b are light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecules are cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Product Categories/Family for anti-MAP1LC3B antibody

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction; Autophagy

References

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
Greenberg JT. Dev Cell. 8(6):799-801. (2005)
Levine B. Cell. 120(2):159-62. (2005)
Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
He H., et al. J. Biol. Chem. 278:29278-29287(2003)
Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 12383056
• NCBI GeneID: 81631
• NCBI Accession #: NP_073729.1
• NCBI GenBank Nucleotide #: NM_022818.4
• UniProt Accession #: Q9GZQ8; Q6NW02
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3B
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 beta
• NCBI Official Symbol: MAP1LC3B
• NCBI Official Synonym Symbols: LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3B
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3B
• UniProt Synonym Gene Names: MAP1ALC3; MAP1A/MAP1B LC3 B
• UniProt Entry Name: MLP3B_HUMAN

NCBI Description

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Uniprot Description

LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome.

Protein type: Autophagy; Microtubule-binding; Vesicle; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 16q24.2

Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; axoneme; cytoplasmic vesicle; intracellular; cytosol

Molecular Function: protein binding; microtubule binding; GABA receptor binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation