Testing Data
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric)
Synonyms
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate); OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric); OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) assay kit
Preparation and Storage
Store the entire kit at -80 degree C. Avoid multiple freeze/thaws by aliquoting. The Enzyme Cofactor is light sensitive and should be maintained in amber tubes
Testing Data
Testing Data
Testing Data #2
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Testing Data #3
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Related Product Information for OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) assay kit
Background: Reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) work together to damage cells and have been implicated in the pathogenesis of several disease states. RNI are a family of molecules derived from nitric oxide (NO) and superoxide anion (O2-), produced via nitric oxide synthase (NOS) and NADPH oxidase, respectively. Nitric oxide is an established mediator in vascular diseases, diabetes, renal ischemia, atherosclerosis, inflammatory diseases, and cancer. However, because of its extremely short half life, direct quantitation of NO production remains challenging and unsuitable for most detection systems. Therefore, the common method for measurement of total NO has become the sum of its final oxidized products, nitrite (NO2-) and nitrate (NO3-) (Figure 1). OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme (Figure 2). Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 muM (in 50 muL sample volume).
Product Categories/Family for OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) assay kit
References
1. Moncada, S. (1992) Acta Physiol. Scand. 145, 201-227.
2. Nathan, C. (1992) FASEB J. 6, 3051-3064.
3. Schreck, R. and Baeuerle, P.A. (1991) Trends Cell Biol. 1, 39-42.
4. Ignarro, L.J. (1990) Pharmacol. Toxicol. 67, 1-7.
5. Green, L.C., et al. (1982) Anal. Biochem. 126, 131-138.
2. Nathan, C. (1992) FASEB J. 6, 3051-3064.
3. Schreck, R. and Baeuerle, P.A. (1991) Trends Cell Biol. 1, 39-42.
4. Ignarro, L.J. (1990) Pharmacol. Toxicol. 67, 1-7.
5. Green, L.C., et al. (1982) Anal. Biochem. 126, 131-138.
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Product Notes
The OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) (Catalog #AAA168933) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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