Human ROMO1 ELISA Kit | ROMO1 elisa kit

Human ROMO1(Reactive oxygen species modulator 1)ELISA Kit

Cat. Num. AAA2515649

• Gene Names: ROMO1; MTGM; MTGMP; C20orf52; bA353C18.2
• Reactivity: Human
• Synonyms: ROMO1; Human ROMO1(Reactive oxygen species modulator 1)ELISA Kit; ROMO1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanROMO1. No significant cross-reactivity or interference between HumanROMO1 and analogues was observed.
• Sequence Length: 79

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ROMO1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanROMO1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ROMO1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ROMO1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ROMO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ROMO1.You can calculate the concentration of ROMO1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18152785
• NCBI GeneID: 140823
• NCBI Accession #: NP_542786.1
• NCBI GenBank Nucleotide #: NM_080748.2
• Molecular Weight: 5,900 Da
• NCBI Official Full Name: reactive oxygen species modulator 1
• NCBI Official Synonym Full Names: reactive oxygen species modulator 1
• NCBI Official Symbol: ROMO1
• NCBI Official Synonym Symbols: MTGM; MTGMP; C20orf52; bA353C18.2
• NCBI Protein Information: reactive oxygen species modulator 1; PCM19; ROS modulator 1; epididymis tissue protein Li 175; glyrichin; mitochondrial targeting GXXXG protein; mitochondrial targeting GxxxG motif protein; protein MGR2 homolog
• UniProt Protein Name: Reactive oxygen species modulator 1
• Protein Family: Reactive oxygen species modulator
• UniProt Gene Name: ROMO1
• UniProt Synonym Gene Names: C20orf52; ROS modulator 1; MTGM
• UniProt Entry Name: ROMO1_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial membrane protein that is responsible for increasing the level of reactive oxygen species (ROS) in cells. The protein also has antimicrobial activity against a variety of bacteria by inducing bacterial membrane breakage. [provided by RefSeq, Nov 2014]

Uniprot Description

ROMO1: Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Belongs to the MGR2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Biological Process: defense response to bacterium; positive regulation of cell proliferation; replicative cell aging