Western Blot (WB)
(Western blot analysis of extracts of Rat brain tissue sample, using ROS Antibody(MBS9603202).)
Rabbit anti-Human, Rat ROS Polyclonal Antibody | anti-ROS antibody
ROS Antibody
Gene Names
ROS1; ROS; MCF3; c-ros-1
Reactivity
Human, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ROS; Polyclonal Antibody; ROS Antibody; c ros 1; c ros; c ros oncogene 1 receptor tyrosine kinase; c Ros receptor tyrosine kinase; c ros1; MCF 3; MCF3; Oncogene ROS; OTTHUMP00000017814; OTTHUMP00000017815; OTTHUMP00000040389; Proto oncogene c ros 1 protein; Proto oncogene c Ros; Proto oncogene tyrosine protein kinase ROS; Proto oncogene tyrosine protein kinase ROS precursor; Proto-oncogene c-Ros-1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c ros oncogene 1; Ros 1; ROS 1C; ROS proto oncogene 1; receptor tyrosine kinase; ROS_HUMAN; ROS1; ROS1C; Transmembrane tyrosine specific protein kinase; v ros avian UR2 sarcoma virus oncogene homolog 1; v ros UR2 sarcoma virus oncogene homolog 1; anti-ROS antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ROS antibody detects endogenous levels of ROS
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
2347
Applicable Applications for anti-ROS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human ROS
Subcellular Location
Membrane.
Tissue Specificity
Expressed in brain. Expression is increased in primary gliomas.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of Rat brain tissue sample, using ROS Antibody(MBS9603202).)
Western Blot (WB)
(Western blot analysis of extracts of Rat brain tissue sample, using ROS Antibody(MBS9603202).)
Related Product Information for anti-ROS antibody
Function: Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2.
Subunit Structure: Interacts with PTPN6 (via SH2 1 domain); the interaction is direct and promotes ROS1 dephosphorylation (By similarity). Interacts with PTPN11; may activate the PI3 kinase-mTOR signaling pathway. Interacts with VAV3; constitutive interaction mediating VAV3 phosphorylation.
Post-translational Modifications: Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11.
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Subunit Structure: Interacts with PTPN6 (via SH2 1 domain); the interaction is direct and promotes ROS1 dephosphorylation (By similarity). Interacts with PTPN11; may activate the PI3 kinase-mTOR signaling pathway. Interacts with VAV3; constitutive interaction mediating VAV3 phosphorylation.
Post-translational Modifications: Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11.
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 263 kD
Predicted: 264 kDa
Predicted: 264 kDa
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase ROS
NCBI Official Synonym Full Names
ROS proto-oncogene 1, receptor tyrosine kinase
NCBI Official Symbol
ROS1
NCBI Official Synonym Symbols
ROS; MCF3; c-ros-1
NCBI Protein Information
proto-oncogene tyrosine-protein kinase ROS
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase ROS
Protein Family
UniProt Gene Name
ROS1
UniProt Synonym Gene Names
MCF3; ROS
NCBI Description
This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2.
Research Articles on ROS
Similar Products
Product Notes
The ROS ros1 (Catalog #AAA9603202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROS Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ROS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ROS ros1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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