Western Blot (WB) (Western blotAll lanes: PRDX5 antibody at 4ug/mlLane 1:mouse brain tissueLane 2:Hela whole cell lysateLane 3:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 23,18,13 kDaObserved band size: 23 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS1496160 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human epityphlon using MBS1496160 at dilution 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of PC3 cells using MBS1496160 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: PRDX2 antibody at 9ug/mlLane 1: Mouse liver tissueLane 2: Mouse spleen tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 22,16 kDaObserved band size: 22 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS7046127 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7046127 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7046127 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western BlotPositive WB detected in:Hela whole cell lysate,HepG2 whole cell lysate,MCF-7 whole cell lysate,Mouse heart tissue,Mouse liver tissue,Mouse brain tissue,Mouse lung tissue,Mouse kidney tissue,Mouse stomach tissueAll lanes: PRDX3 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 28,26 kDaObserved band size: 28 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer tissue tissue using MBS7048919 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human renal tissue using MBS7048919 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7048919 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunoprecipitation (IP) (Immunoprecipitating PRDX3 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of MBS7048919 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7048919 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Western Blot (WB) (Western blotAll lanes: PRDX4 antibody at 8ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 31kDaObserved band size: 31kDa)
Western Blot (WB) (Western Blot analysis of Hela, 293T and MCF7 cell, Human fetal brain tissue and Jurkat cell using PRDX3 Polyclonal Antibody at dilution of 1:800)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human breast cancer using PRDX3 Polyclonal Antibody at dilution of 1:50)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using PRDX3 Polyclonal Antibody at dilution of 1:50)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Placenta stained with Superoxide Dismutase 1 Mouse Monoclonal Antibody (SOD1/2089).)
SDS-Page (SDS-PAGE Analysis Purified Superoxide Dismutase 1 Mouse Monoclonal Antibody (SOD1/2089).Confirmation of Integrity and Purity of Antibody.)
Testing Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using Superoxide Dismutase 1 Mouse Monoclonal Antibody (SOD1/2089).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-Page (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Western Blot (WB) (Western blot analysis of extracts from mouse brain, using PRDX6 Antibody.)
Western Blot (WB) (Western blot analysis of Mouse brain lysate, using PRDX6 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of extracts from various samples, using Peroxiredoxin 6 Ab.Lane 1: Rat lung, blocked with antigen-specific peptides,Lane 2: Rat lung,Lane 3: Mouse liver.)
Immunohistochemistry (IHC) (MBS9607421 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9607421 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Immunofluorescence (IF) (MBS9607421 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9607421 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.The nuclear counter stain is DAPI(blue).)
Western Blot (WB) (Western blot analysis of extracts of mouse liver tissue, using PRX5 antibody.)
Immunohistochemistry (IHC) (MBS9603535 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PRDX6 at 1:1000 dilution.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung cancer using PRDX6 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human stomach cancer using PRDX6 antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using PRDX6 antibody.)
Testing Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Peroxiredoxin 4 Monoclonal Antibody (CPTC-PRDX4-2).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ATOX1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using ATOX1 antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western blot analysis of Peroxiredoxin 3 expression in HeLa (A), MCF7 (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of Peroxiredoxin 3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of Peroxiredoxin 3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PRDX5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using PRDX5 antibody at dilution of 1:200 (40x lens).)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PRDX2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using PRDX2 antibody.)
Immunoprecipitation (IP) (PRDX3 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25079 of 10 ug. Western blot was performed from the immunopre-cipitate using M25079 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 27 KDaObserved band size : 27 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PRDX3 with M25079 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Western Blot (WB) (Anti- Peroxiredoxin 5 Picoband antibody, MBS177800, Western blottingAll lanes: Anti Peroxiredoxin 5 (MBS177800) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 22KDObserved bind size: 22KD)
Immunohistochemistry (IHC) (MBS9607082 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of Mouse lung lysate, using PRDX4 Antibody. The lane on the left is treated with the antigen-specific peptide.)
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