Typical Testing Data/Standard Curve (for reference only)
Ferric Reducing Antioxidant Power Assay Kit | FRAP assay kit
OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit
Synonyms
Ferric Reducing Antioxidant Power; OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit; FRAP assay kit
Preparation and Storage
Upon receipt, store the kit components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FRAP assay kit
Principle of the Assay: The OxiSelect™ Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within a sample. Ferric iron (Fe3+) is initially reduced by electron-donating antioxidants present within the sample to its ferrous form (Fe2+). The iron-colorimetric probe complex develops a dark blue color product upon reduction which can be measured at 540-600 nm (see Figure 1). Samples can be compared to the iron standard for determining antioxidant potential. This assay is analytically sensitive to approximately 4 uM of Fe2+ iron equivalents, or a FRAP value of 2.
Background/Introduction: Oxidative stress is a physiological condition where there is an imbalance between concentrations of reactive oxygen species (ROS) and antioxidants. However, excessive ROS accumulation will lead to inflammation and cellular injury, such as damage to DNA, proteins, and lipid membranes. The cellular damage caused by ROS has been implicated in the development of many disease states, such as cancer, diabetes, cardiovascular disease, atherosclerosis, and neurodegenerative diseases. Under normal physiological conditions, cellular ROS generation is counterbalanced by the action of cellular antioxidant enzymes, macro or micro molecules, as well as other redox molecules. Antioxidants are molecules that contain one or more free electrons that can be donated to stabilize ROS. Antioxidants include both hydrophilic and lipophilic molecules for metabolizing ROS. These may be localized transiently within different tissues or cells. Because of their potential harmful effects, excessive ROS must be promptly neutralized and eliminated from the cells by this variety of antioxidant defense mechanisms. OxiSelect™ Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples such as serum, plasma, urine, saliva, tears, tissue homogenates, cell extracts, and purified food or drug extracts. The assay is based on the highly-cited work of Benzie and Strain (ref. 1 and 2) in which iron reacts with a colorimetric probe to produce a blue product. The reaction is driven by the electron donating reducing power of antioxidants. The kit employs a ferrous iron standard which allows the user to determine the antioxidant content present within their sample. Each kit provides sufficient reagents to perform up to 200 assays, including standard curve and unknown samples. The FRAP assay offers a simple and efficient analytical method for assessing age, disease, diet, or other physiological changes to antioxidant status.
Background/Introduction: Oxidative stress is a physiological condition where there is an imbalance between concentrations of reactive oxygen species (ROS) and antioxidants. However, excessive ROS accumulation will lead to inflammation and cellular injury, such as damage to DNA, proteins, and lipid membranes. The cellular damage caused by ROS has been implicated in the development of many disease states, such as cancer, diabetes, cardiovascular disease, atherosclerosis, and neurodegenerative diseases. Under normal physiological conditions, cellular ROS generation is counterbalanced by the action of cellular antioxidant enzymes, macro or micro molecules, as well as other redox molecules. Antioxidants are molecules that contain one or more free electrons that can be donated to stabilize ROS. Antioxidants include both hydrophilic and lipophilic molecules for metabolizing ROS. These may be localized transiently within different tissues or cells. Because of their potential harmful effects, excessive ROS must be promptly neutralized and eliminated from the cells by this variety of antioxidant defense mechanisms. OxiSelect™ Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples such as serum, plasma, urine, saliva, tears, tissue homogenates, cell extracts, and purified food or drug extracts. The assay is based on the highly-cited work of Benzie and Strain (ref. 1 and 2) in which iron reacts with a colorimetric probe to produce a blue product. The reaction is driven by the electron donating reducing power of antioxidants. The kit employs a ferrous iron standard which allows the user to determine the antioxidant content present within their sample. Each kit provides sufficient reagents to perform up to 200 assays, including standard curve and unknown samples. The FRAP assay offers a simple and efficient analytical method for assessing age, disease, diet, or other physiological changes to antioxidant status.
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Product Notes
The FRAP (Catalog #AAA169262) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ferric Reducing Antioxidant Power, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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