Rabbit Peroxiredoxin 5 Polyclonal Antibody | anti-PRDX5 antibody

Anti-Peroxiredoxin 5 antibody

Cat. Num. AAA176336

• Gene Names: PRDX5; PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
• Reactivity: Human, Rat; Predicted to work with mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Peroxiredoxin 5; Polyclonal Antibody; Anti-Peroxiredoxin 5 antibody; Peroxiredoxin-5; mitochondrial; peroxiredoxin 5; ACR 1 antibody; ACR1 antibody; Alu co repressor 1 antibody; Alu co-repressor 1 antibody; Alu corepressor 1 antibody; antioxidant enzyme B166 antibody; AOEB166 antibody; B166 antibody; liver tissue 2D-page spot 71B antibody; MGC117264 antibody; MGC142283 antibody; MGC142285 antibody; mitochondrial antibody; Peroxiredoxin 5 mitochondrial antibody; Peroxiredoxin V antibody; Peroxiredoxin-5 antibody; Peroxiredoxin5 antibody; peroxisomal antioxidant enzyme antibody; PLP antibody; PMP 20 antibody; PMP20 antibody; PRDX 5 antibody; PRDX 6 antibody; PRDX5 antibody; PRDX5_HUMAN antibody; PRDX6 antibody; Prx V antibody; Prx-V antibody; PRXV antibody; SBBI10 antibody; thioredoxin peroxidase PMP20 antibody; Thioredoxin reductase antibody; TPx type VI antibody; anti-PRDX5 antibody

• Host: Rabbit
• Reactivity: Human, Rat; Predicted to work with mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 214

• Applicable Applications for anti-PRDX5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot:; Concentration: 0.1-0.5ug/ml; Tested Species:Hu, Rat; Predicted Species: Ms; Antigen Retrieval: N/A; Immunohistochemistry (Paraffin embedded Section):; Concentration: 0.5-1ug/ml; Tested Species:Hu, Rat; Predicted Species: Ms; Antigen Retrieval: by Heat; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin section.; Other applications have not been tested.; Optimal dilutions to be determined by end user.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 5 (166-181aa DDSLVSIFGNRRLKRF), identical to the related mouse and rat sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN₃.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Peroxiredoxin 5 antibody, MBS176336, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Lung Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Kidney Tissue LysateLane 5: HELA Cell LysateLane 6: 293T Cell LysateLane 7: MCF-7 Cell LysateLane 8: A549 Cell Lysate)

Immunohistochemistry (IHC)

(Anti-Peroxiredoxin 5 antibody, MBS176336, IHC(P)IHC(P): Human Prostatic Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-Peroxiredoxin 5 antibody, MBS176336, IHC(P)IHC(P): Rat Liver Tissue )

Related Product Information for anti-PRDX5 antibody

Description: Rabbit IgG polyclonal antibody for Peroxiredoxin-5, mitochondrial(PRDX5) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: PRDX5(peroxiredoxin 5) also known as AOEB166, ACR1,B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped on 11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.

NCBI and Uniprot Product Information

• NCBI GI #: 317373539
• NCBI GeneID: 25824
• UniProt Accession #: P30044; Q14CK0; Q6IAF2; Q9UBU5; Q9UJU4; Q9UKX4; A6NC19; A6NG06; B7ZLJ4; B7ZVW3
• Molecular Weight: 12,897 Da
• NCBI Official Full Name: Peroxiredoxin-5, mitochondrial
• NCBI Official Synonym Full Names: peroxiredoxin 5
• NCBI Official Symbol: PRDX5
• NCBI Official Synonym Symbols: PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
• NCBI Protein Information: peroxiredoxin-5, mitochondrial; TPx type VI; peroxiredoxin V; Alu co-repressor 1; thioredoxin reductase; antioxidant enzyme B166; thioredoxin peroxidase PMP20; liver tissue 2D-page spot 71B; peroxisomal antioxidant enzyme; epididymis secretory protein Li 55
• UniProt Protein Name: Peroxiredoxin-5, mitochondrial
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX5
• UniProt Synonym Gene Names: ACR1; AOEB166; Prx-V
• UniProt Entry Name: PRDX5_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: peroxisomal matrix; extracellular space; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; mitochondrial matrix; cytoplasm; peroxisome; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: antioxidant activity; protein dimerization activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; receptor binding

Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; negative regulation of transcription from RNA polymerase III promoter; positive regulation of collagen biosynthetic process; negative regulation of oxidoreductase activity; response to oxidative stress; negative regulation of caspase activity; inflammatory response; negative regulation of apoptosis