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Testing Data
Antioxidant Assay Kit
Total Antioxidant Power Kit (Replaces TA01)
Synonyms
Antioxidant; Total Antioxidant Power Kit (Replaces TA01); Total Antioxidant Power Kit; Antioxidant assay kit
Sequence Length
207
Preparation and Storage
1. Store the components of this kit at the temperatures specified on the labels.
2. Unopened reagents are stable until the indicated kit expiration date.
3. Aliquot the Trolox Standard after reconstitution and freeze at At -80 degree C.
2. Unopened reagents are stable until the indicated kit expiration date.
3. Aliquot the Trolox Standard after reconstitution and freeze at At -80 degree C.
Testing Data
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Related Product Information for Antioxidant assay kit
Principle of the Assay: The reduction potential of the sample or standard effectively converts Cu+2 to Cu+1, thus changing the ion's absorption characteristics. This reduced form of copper will selectively form a stable 2:1 complex with the chromogenic reagent with an absorption maximum at ca. 450 nm12-16. A known concentration of Trolox is used to create a calibration curve, with the data being expressed as mM Trolox equivalents or in uM copper reducing equivalents. A study was performed to evaluate the correlation between the concentration of principal antioxidants in human serum and the value obtained for total antioxidant power using this method with the results presented in Figure 1. Multivariate analysis of the obtained results yields a very high significance. The results obtained with the Total Antioxidant Power Assay for a series of serum samples were also compared to the resistance to oxidation of the serum lipids in these samples. The results of this study also show that these two parameters are highly correlated. The higher the total antioxidant power, the more protected the serum lipids are from oxidation.
Background/Introduction: Oxidative stress has been implicated in a number of diseases such as atherosclerosis1, chronic inflammatory disease2, chronic renal failure3, and cancer4. It is a condition where an imbalance exists between the production of reactive oxidizing species and the body's ability to neutralize these intermediates, resulting in cellular damage. The body has designed several physiological responses to oxidative stress including counterbalances5 such as enzymes and variously functionalized molecules (see examples below) that effectively neutralize these damaging species. These antioxidants can be either water or lipid soluble, and are localized transiently throughout various tissues, cells and cell types. Given the multiplicity of antioxidant pathways, their centrality in the prevention of oxidant stress, and the influences of lifestyle and nutritional supplements on an individual's antioxidant capacity, it is important to be able to quantitatively measure the total antioxidant capacity or antioxidant power with biological specimens6-11.
Background/Introduction: Oxidative stress has been implicated in a number of diseases such as atherosclerosis1, chronic inflammatory disease2, chronic renal failure3, and cancer4. It is a condition where an imbalance exists between the production of reactive oxidizing species and the body's ability to neutralize these intermediates, resulting in cellular damage. The body has designed several physiological responses to oxidative stress including counterbalances5 such as enzymes and variously functionalized molecules (see examples below) that effectively neutralize these damaging species. These antioxidants can be either water or lipid soluble, and are localized transiently throughout various tissues, cells and cell types. Given the multiplicity of antioxidant pathways, their centrality in the prevention of oxidant stress, and the influences of lifestyle and nutritional supplements on an individual's antioxidant capacity, it is important to be able to quantitatively measure the total antioxidant capacity or antioxidant power with biological specimens6-11.
References
1. Brook, R.; (2008) Clin. Sci. (Lond). 115:175-187
2. Cerutti, P. and Trump, B.; (1991) Cancer Cell 3:1-7
3. Cottone, S., et al.; (2008) J. Nephrol. 21:175-179
4. Trachootham, D., et al.; (2008) Antioxid. Redox Signal. 10:1343-1374
5. Frei, B., et al.; (1992) Molecular Biology of Free Radical Scavenging System 23-45
6. Ciencewicki, J., et al.; (2008) J. Allergy Clin. Immunol. 122:456-468
7. Kieth, M., et al.; (1998) J. Am. Coll. Cardio. 31:1352-1362
8. Van-Zoeren-Grobben, et al.; (1997) Acta Pediatrics 86:1356-1362
9. Allard, J.P., et al.; (1998) Am. J. Clin. Nutr. 67:143-147
10. Santini S.A., et al.; (1997) Diabetes 46:1853-1858
11. Fatokun, A., Stone, T. and Smith, R.; (2008) Front. Biosci. 13:3288-3311
1
2. Apak, R., et al.; (2004) J Agric Food Chem 52:7970-7981
1
3. Apak, R., et al.; (2005) Free Radic Res 39:949-961
1
4. Apak, R., et al.; (2007) Molecules 12:1496-1547
1
5. Apak, R., et al.; (2008) Microchim Acta 160:413-419
1
6. Apak, R., et al.; (2008) Anal. Chim. Acta 630:28-39
2. Cerutti, P. and Trump, B.; (1991) Cancer Cell 3:1-7
3. Cottone, S., et al.; (2008) J. Nephrol. 21:175-179
4. Trachootham, D., et al.; (2008) Antioxid. Redox Signal. 10:1343-1374
5. Frei, B., et al.; (1992) Molecular Biology of Free Radical Scavenging System 23-45
6. Ciencewicki, J., et al.; (2008) J. Allergy Clin. Immunol. 122:456-468
7. Kieth, M., et al.; (1998) J. Am. Coll. Cardio. 31:1352-1362
8. Van-Zoeren-Grobben, et al.; (1997) Acta Pediatrics 86:1356-1362
9. Allard, J.P., et al.; (1998) Am. J. Clin. Nutr. 67:143-147
10. Santini S.A., et al.; (1997) Diabetes 46:1853-1858
11. Fatokun, A., Stone, T. and Smith, R.; (2008) Front. Biosci. 13:3288-3311
1
2. Apak, R., et al.; (2004) J Agric Food Chem 52:7970-7981
1
3. Apak, R., et al.; (2005) Free Radic Res 39:949-961
1
4. Apak, R., et al.; (2007) Molecules 12:1496-1547
1
5. Apak, R., et al.; (2008) Microchim Acta 160:413-419
1
6. Apak, R., et al.; (2008) Anal. Chim. Acta 630:28-39
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
antioxidant
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Product Notes
The Antioxidant (Catalog #AAA480477) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Antioxidant, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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