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Reactivity
Gene Name
Isotype
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Application
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164 results for "Immunity Inflammation" - showing 1-50
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (SC-05).)
IgA Secretory Component / ECM1, Monoclonal Antibody (Cat# AAA438279)
Full Name
IgA Secretory Component / ECM1 Mouse Monoclonal Antibody
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Sequence)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA2086081)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>95%
Pricing
$250/0.01 mg | $545/0.05 mg | $845/0.1 mg | $1,045/0.2 mg | $2,045/0.5 mg | $3,025/1 mg | $7,405/5 mg
GAPDH, Polyclonal Antibody (Cat# AAA9205368)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Figure 1. Western blot analysis of RANK using anti- RANK antibody (MBS1750556).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: HEPG2 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- RANK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANK at approximately 80KD. The expected band size for RANK is at 66KD. )
RANK, Polyclonal Antibody (Cat# AAA1750556)
Full Name
Anti-RANK Picoband Antibody
Gene Names
TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-paraffin human Colon Carcinoma stained with IgA Secretory Component Mab (SPM217).)
IgA Secretory Component / ECM1, Monoclonal Antibody (Cat# AAA438012)
Full Name
IgA Secretory Component / ECM1 Mouse Monoclonal Antibody
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (ECM1/792).)
IgA Secretory Component / ECM1, Monoclonal Antibody (Cat# AAA438891)
Full Name
IgA Secretory Component / ECM1 Mouse Monoclonal Antibody
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105113)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
Mif; GIF; DER6; Glif
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Sequence
Galectin 3 (GAL3), Active Protein (Cat# AAA2105089)
Full Name
Active Galectin 3 (GAL3)
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>98%
Pricing
$185/0.01 mg | $355/0.05 mg | $540/0.1 mg | $665/0.2 mg | $1,285/0.5 mg | $1,915/1 mg | $4,695/5 mg
Activity Data
(Figure. Cell proliferation of TF-1 cells after stimulated with LIF.Leukemia Inhibitory Factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. To test the effect of LIF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human LIF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with LIF for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant LIF for 72h. The result was shown in Figure 2. It was obvious that LIF significantly increased cell viability of TF-1 cells.(A) TF-1 cells cultured in 1640, stimulated with 0.5ng/mL LIF for 72h; (B) Unstimulated TF-1 cells cultured in 1640 for 72h.)
Leukemia Inhibitory Factor (LIF), Active Protein (Cat# AAA2097262)
Full Name
Active Leukemia Inhibitory Factor (LIF)
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $310/0.05 mg | $480/0.1 mg | $585/0.2 mg | $1,120/0.5 mg | $1,660/1 mg | $4,075/5 mg
Western Blot (WB)
Myeloperoxidase, Polyclonal Antibody (Cat# AAA540041)
Full Name
Myeloperoxidase Antibody
Reactivity
Human, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105112)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Activity Data
(Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.Result: MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6, the data was shown in Figure 1.)
Interleukin 6 (IL6), Active Protein (Cat# AAA2097342)
Full Name
Active Interleukin 6 (IL6)
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Activity Data
(Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.)
Growth Regulated Oncogene Beta (GROb), Active Protein (Cat# AAA2097295)
Full Name
Active Growth Regulated Oncogene Beta (GROb)
Gene Names
Cxcl2; Mip-2; Scyb2
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Immunohistochemistry (IHC)
CD163, Polyclonal Antibody (Cat# AAA540877)
Full Name
CD163 Antibody
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human, Monkey
Applications
EIA, IHC, IF, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Secretory Component Rabbit Recombinant Monoclonal (ECM1/2889R).)
Secretory Component/ECM1, Monoclonal Antibody (Cat# AAA4380544)
Full Name
Secretory Component/ECM1
Gene Names
ECM1; URBWD
Reactivity
Human, Rat. Others not known.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (MBS1750500).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD. )
PF4/Cxcl4, Polyclonal Antibody (Cat# AAA1750500)
Full Name
Anti-PF4/Cxcl4 Picoband antibody
Gene Names
Pf4; PF-4; Pf4a; Cxcl4; RATPF4A
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Sequence
High Mobility Group Protein 1 (HMG1), Active Protein (Cat# AAA2105091)
Full Name
Active High Mobility Group Protein 1 (HMG1)
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>95%
Pricing
$250/0.01 mg | $555/0.05 mg | $865/0.1 mg | $1,065/0.2 mg | $2,095/0.5 mg | $3,100/1 mg | $7,590/5 mg
Western Blot (WB)
(Western blot analysis of BRI3 using Jurkat whole cell lysates)
BRI3, Polyclonal Antibody (Cat# AAA9609601)
Full Name
BRI3 Antibody
Gene Names
BRI3; I3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6, the data was shown in Figure 1.)
Interleukin 6 (IL6), Active Protein (Cat# AAA2097327)
Full Name
Active Interleukin 6 (IL6)
Gene Names
IL6; IL-6; CHIL-6; interleukin-6
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $310/0.05 mg | $480/0.1 mg | $585/0.2 mg | $1,120/0.5 mg | $1,660/1 mg | $4,075/5 mg
Western Blot (WB)
(The anti-HK1 Pab is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.)
HK1 (Hexokinase), Polyclonal Antibody (Cat# AAA9204225)
Full Name
HK1 (Hexokinase) Antibody (N-term)
Gene Names
HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Rat
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (MBS1750574).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD40L at approximately 36KD. The expected band size for CD40L is at 29KD. )
CD40L, Polyclonal Antibody (Cat# AAA1750574)
Full Name
Anti-CD40L Picoband antibody
Gene Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
CD163, Polyclonal Antibody (Cat# AAA540774)
Full Name
CD163 Antibody C-epitope
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human, Monkey
Applications
EIA, IF, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Activity Data
(IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.)
Interleukin 20 (IL20), Active Protein (Cat# AAA2097236)
Full Name
Active Interleukin 20 (IL20)
Gene Names
IL20; IL-20; IL10D; ZCYTO10
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$185/0.01 mg | $360/0.05 mg | $550/0.1 mg | $675/0.2 mg | $1,305/0.5 mg | $1,940/1 mg | $4,765/5 mg
Western Blot (WB)
TLR10, Polyclonal Antibody (Cat# AAA540461)
Full Name
TLR10 Antibody
Gene Names
TLR10; CD290
Reactivity
Chimpanzee, Human, Monkey, Pig, Rat
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Sequence
Elastase 2, Neutrophil, Active Protein (Cat# AAA2105086)
Full Name
Active Elastase 2, Neutrophil (ELA2)
Applications
Cell culture, Activity Assays
Purity
> 98%
Pricing
$185/0.01 mg | $355/0.05 mg | $540/0.1 mg | $665/0.2 mg | $1,285/0.5 mg | $1,915/1 mg | $4,695/5 mg
Activity Data
(Macrophage migration inhibitory factor (MIF), also known as glycosylation- inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG- coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MIF with MHCDG.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105111)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-alpha-4 integrin antibody 21.6 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
alpha-4 Integrin, Monoclonal Recombinant Antibody (Cat# AAA488448)
Full Name
Anti-alpha-4 Integrin [21.6]
Gene Names
ITGA4; IA4; CD49D
Reactivity
Human
Applications
EIA, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-alpha-4 integrin antibody 21.6 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 (MBS488449) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488449, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
alpha-4 Integrin, Monoclonal Recombinant Antibody (Cat# AAA488449)
Full Name
Anti-alpha-4 Integrin [21.6]
Gene Names
ITGA4; IA4; CD49D
Reactivity
Human
Applications
EIA, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)-Formallin
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Mouse Monoclonal Antibody (rECM1/792).)
Secretory Component/ECM1, Monoclonal Antibody (Cat# AAA4381537)
Full Name
Secretory Component/ECM1
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)-Formallin
(Formalin-fixed, paraffin-embedded human liver stained with ECM1 Recombinant Mouse Monoclonal Antibody (rSPM217). )
Secretory Component/ECM1, Monoclonal Antibody (Cat# AAA4381630)
Full Name
Secretory Component/ECM1
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
OPG, Polyclonal Antibody (Cat# AAA535773)
Full Name
OPG antibody
Gene Names
TNFRSF11B; OPG; TR1; OCIF; PDB5
Reactivity
human OPG
Applications
EIA, WB
Pricing
$425/0.05 mg | $1,905/5x0.05 mg
IFI27, Antibody (Cat# AAA540037)
Full Name
IFI27 Antibody
Gene Names
IFI27; P27; ISG12; FAM14D; ISG12A
Reactivity
Human
Applications
EIA, IMM, IHC, WB, DB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
TLR5, Polyclonal Antibody (Cat# AAA540533)
Full Name
TLR5 Antibody
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Cat, Human, Mouse, Pig
Applications
EIA, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
TLR11, Polyclonal Antibody (Cat# AAA540124)
Full Name
TLR11 Antibody Affinity Purified
Gene Names
Tlr11; Gm287
Reactivity
Chimpanzee, Human, Monkey
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
IL-25, Monoclonal Antibody (Cat# AAA670216)
Full Name
Mouse Anti-Human IL-25-UNLB
Gene Names
IL25; IL17E
Reactivity
Human
Applications
ELISA
Pricing
$365/0.5 mg | $1,495/5x0.5 mg
Immunoassay (IA)
(The calibration curve of a sandwich assay for measurement of C3a(desArg) using MBS838793 as the capture antibody and MBS838118 as the biotinylated detection antibody.)
C3a desArg, Monoclonal Antibody (Cat# AAA838793)
Full Name
C3a desArg antibody
Reactivity
Human
Applications
EIA, WB
Purity
C3a desArg antibody was purified from cell culture supernatant by protein A/G chromatography
Pricing
$1,765/0.4 mL | $7,935/5x0.4 mL
Immunohistochemistry (IHC)
TLR13, Polyclonal Antibody (Cat# AAA5400086)
Full Name
TLR13 Antibody
Gene Names
Tlr13; Gm713; AI666735
Reactivity
Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Flow Cytometry (FC/FACS)
(Staining of non-transfected (left) and human CD289-transfected (right) HEK 293T cells with 1.0 ug of Rat IgG2a kappa Isotype Control (PE) (open histogram) or 1.0 ug of CD289 antibody (PE) (filled histogram). Total viable cells were used for analysis.)
Toll-like receptor 9, Monoclonal Antibody (Cat# AAA833532)
Full Name
Toll-like receptor 9 antibody (PE)
Applications
FC/FACS
Pricing
$1,010/0.1 mg | $4,545/5x0.1 mg
CCR2, Polyclonal Antibody (Cat# AAA540147)
Full Name
CCR2 Antibody
Reactivity
Human
Applications
EIA, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Flow Cytometry (FC/FACS)
(Staining of thioglycolate-induced peritoneal exudate cells with TLR4/MD-2 Complex antibody (PE). Appropriate Isotype Controls were used (open histogram). Cells in the large scatter population were used for analysis.)
Toll-like receptor 4, Monoclonal Antibody (Cat# AAA834441)
Full Name
Toll-like receptor 4 antibody
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
User optimized
Purity
Toll-like receptor 4 antibody was purified by affinity chromatography.
Pricing
$365/0.05 mg | $1,625/5x0.05 mg
Western Blot (WB)
(Cell lysates of HeLa(40 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IRF3, Monoclonal Antibody (Cat# AAA831100)
Full Name
IRF3 antibody
Reactivity
Human
Applications
EIA, IF, IP, WB
Purity
IRF3 antibody was purified by protein-G affinity chromatography
Pricing
$435/0.1 mL | $1,945/5x0.1 mL
IL23 P19, Polyclonal Antibody (Cat# AAA540708)
Full Name
IL23 P19 Antibody
Gene Names
IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control (PE) (open histogram) or CD284 antibody (PE) (filled histogram). Cells in the monocyte gate were used for analysis.)
Toll-like receptor 4, Monoclonal Antibody (Cat# AAA832508)
Full Name
Toll-like receptor 4 antibody (PE)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
FC/FACS
Pricing
$755/100 Tests | $3,400/5x100 Tests
Western Blot (WB)
TLR9, Polyclonal Antibody (Cat# AAA540152)
Full Name
TLR9 Antibody
Gene Names
TLR9; CD289
Reactivity
Chimpanzee, Human, Monkey, Rat
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Testing Data #1
(Staining of formalin fixed, paraffin embedded human adrenal medullary pheochromocytes with Rabbit anti Human SPHK1 (MBS221451))
SPHK1, Polyclonal Antibody (Cat# AAA221451)
Full Name
RABBIT ANTI HUMAN SPHK1 (N-TERMINAL)
Gene Names
SPHK1; SPHK
Applications
Immunohistology Paraffin*
Pricing
$625/0.05 mg | $2,670/5x0.05 mg
Western Blot
(Western Blot analysis using TGF beta 1 antibodyWestern Blot showing TGF beta1 antibody used against truncated TGF beta1 recombinant protein.)
TGF beta 1, Monoclonal Antibody (Cat# AAA830707)
Full Name
TGF beta 1 antibody
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Applications
EIA, WB
Pricing
$375/0.1 mL | $1,680/5x0.1 mL
IL-25, Monoclonal Antibody (Cat# AAA670255)
Full Name
Mouse Anti-Human IL-25-UNLB
Gene Names
IL25; IL17E
Reactivity
Human
Applications
ELISA
Pricing
$365/0.5 mg | $1,495/5x0.5 mg
Western Blot (WB)
Lysozyme, Polyclonal Antibody (Cat# AAA540526)
Full Name
Lysozyme Antibody
Gene Names
SPACA3; CT54; LYC3; LYZC; LYZL3; SLLP1; ALLP17
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
TLR7, Polyclonal Antibody (Cat# AAA540166)
Full Name
TLR7 Antibody Affinity Purified
Gene Names
TLR7; TLR7-like
Reactivity
Cat, Human, Mouse, Pig
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Flow Cytometry (FC/FACS)
(Human peripheral blood monocytes were stained with CD282 antibody followed by Anti-Mouse IgG (FITC). (Gated on normal peripheral monocytes))
Toll-like receptor 2, Monoclonal Antibody (Cat# AAA833258)
Full Name
Toll-like receptor 2 antibody
Applications
Functional Assay
Purity
Toll-like receptor 2 antibody was purified by affinity chromatography.
Pricing
$335/0.025 mg | $1,495/5x0.025 mg
