Rabbit SPHK1 Polyclonal Antibody | anti-SPHK1 antibody

RABBIT ANTI HUMAN SPHK1 (N-TERMINAL)

Cat. Num. AAA221451

• Gene Names: SPHK1; SPHK
• Applications: Immunohistochemistry
• Synonyms: SPHK1; Polyclonal Antibody; RABBIT ANTI HUMAN SPHK1 (N-TERMINAL); anti-SPHK1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the N-terminal region human SphK1, also known sphingosine kinase 1. SphK1 catalyzes the phosphorylation of sphingosine 1-phosphate; an important lipid mediator with multiple functions. In light of this, SphK1 has been implicated in regulation of Ca2+ homoeostasis, inflammation, immunity and cancer growth. However, its central function is thought to be in the development of the cardiovascular and central nervous system. SphK1 has a molecular weight of 42kD.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 384

• Applicable Applications for anti-SPHK1 antibody: Immunohistology Paraffin*
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 5; Maximum Dilution: 15ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide: N-terminal domain of human SphK1, conjugated to KLH.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to SphK1 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human adrenal medullary pheochromocytes with Rabbit anti Human SPHK1 (MBS221451))

Related Product Information for anti-SPHK1 antibody

MBS221451 detects an epitope within the N-terminal region human SphK1, also known sphingosine kinase 1. SphK1 catalyzes the phosphorylation of sphingosine 1-phosphate; an important lipid mediator with multiple functions. In light of this, SphK1 has been implicated in regulation of Ca2+ homoeostasis, inflammation, immunity and cancer growth. However, its central function is thought to be in the development of the cardiovascular and central nervous system. SphK1 has a molecular weight of 42kD.

NCBI and Uniprot Product Information

• NCBI GI #: 217272883
• NCBI GeneID: 8877
• NCBI Accession #: NP_001136073.1
• NCBI GenBank Nucleotide #: NM_001142601.1
• UniProt Accession #: Q9NYA1; Q8N632; Q96GK1; Q9HD92; Q9NY70; Q9NYL3
• Molecular Weight: 43,944 Da
• NCBI Official Full Name: sphingosine kinase 1 isoform 3
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1; SK 1; SPK 1
• UniProt Protein Name: Sphingosine kinase 1
• UniProt Gene Name: SPHK1
• UniProt Synonym Gene Names: SPHK; SPK; SK 1; SPK 1
• UniProt Entry Name: SPHK1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.91; Lipid Metabolism - sphingolipid; Kinase, lipid

Chromosomal Location of Human Ortholog: 17q25.2

Cellular Component: synaptic vesicle; axon; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: D-erythro-sphingosine kinase activity; calmodulin binding; protein binding; DNA binding; protein phosphatase 2A binding; sphinganine kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: protein folding; positive regulation of NF-kappaB import into nucleus; female pregnancy; signal transduction; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; activation of NF-kappaB transcription factor; 'de novo' posttranslational protein folding; response to magnesium ion; positive regulation of fibroblast proliferation; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of smooth muscle contraction; inflammatory response; blood vessel development; sphingolipid metabolic process; sphingolipid biosynthetic process; cyclooxygenase pathway; calcium-mediated signaling; sphingosine metabolic process; lipid phosphorylation; cellular response to starvation; positive regulation of cell growth; response to ATP; positive regulation of angiogenesis; cellular protein metabolic process; positive regulation of protein ubiquitination; brain development; positive regulation of protein amino acid phosphorylation; response to progesterone stimulus; vascular endothelial growth factor receptor signaling pathway; sphingoid catabolic process; negative regulation of apoptosis; positive regulation of cell migration; response to amine stimulus