Bovine Adipose Triglyceride Lipase ELISA Kit | ATGL elisa kit

Bovine Adipose Triglyceride Lipase ELISA Kit

Cat. Num. AAA755287

• Gene Names: PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
• Reactivity: Bovine
• Synonyms: Adipose Triglyceride Lipase; Bovine Adipose Triglyceride Lipase ELISA Kit; ATGL elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This ATGL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ATGL. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATGL elisa kit

Principle of the Assay: ATGL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATGL antibody and an ATGL-HRP conjugate. The assay sample and buffer are incubated together with ATGL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATGL concentration since ATGL from samples and ATGL-HRP conjugate compete for the anti-ATGL antibody binding site. Since the number of sites is limited, as more sites are occupied by ATGL from the sample, fewer sites are left to bind ATGL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATGL concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 58759051
• NCBI GeneID: 57104
• Molecular Weight: 19,875 Da
• NCBI Official Full Name: adipose triglyceride lipase
• NCBI Official Synonym Full Names: patatin-like phospholipase domain containing 2
• NCBI Official Symbol: PNPLA2
• NCBI Official Synonym Symbols: ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 2; TTS2.2; desnutrin; IPLA2-zeta; triglyceride hydrolase; adipose triglyceride lipase; transport-secretion protein 2.2; pigment epithelium-derived factor; calcium-independent phospholipase A2; patatin-like phospholipase domain containing protein 2
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 2
• UniProt Gene Name: PNPLA2
• UniProt Synonym Gene Names: ATGL; FP17548; TTS2
• UniProt Entry Name: PLPL2_HUMAN

NCBI Description

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

Uniprot Description

PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipase; Membrane protein, integral; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; lipid particle; cytosol

Molecular Function: triacylglycerol lipase activity

Biological Process: sequestering of lipid; phospholipid metabolic process; glycerophospholipid biosynthetic process; triacylglycerol catabolic process

Disease: Neutral Lipid Storage Disease With Myopathy