Rabbit RPA1 Polyclonal Antibody | anti-RPA1 antibody

RPA1 antibody

Cat. Num. AAA9407737

• Gene Names: RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: RPA1; Polyclonal Antibody; RPA1 antibody; HSSB; MST075; REPA1; RF-A; RP-A; RPA70; anti-RPA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total RPA1 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 616

• Applicable Applications for anti-RPA1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human RPA1.
• Target Name: RPA1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of A431 cell lysate using RPA1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using RPA1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-RPA1 antibody

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol a, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).

Product Categories/Family for anti-RPA1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506583
• NCBI GeneID: 6117
• NCBI Accession #: NP_002936.1
• NCBI GenBank Nucleotide #: NM_002945.3
• UniProt Accession #: P27694; Q59ES9; A8K0Y9
• Molecular Weight: 68,138 Da
• NCBI Official Full Name: replication protein A 70 kDa DNA-binding subunit
• NCBI Official Synonym Full Names: replication protein A1, 70kDa
• NCBI Official Symbol: RPA1
• NCBI Official Synonym Symbols: HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• NCBI Protein Information: replication protein A 70 kDa DNA-binding subunit
• UniProt Protein Name: Replication protein A 70 kDa DNA-binding subunit
• Protein Family: Replication factor
• UniProt Gene Name: RPA1
• UniProt Synonym Gene Names: REPA1; RPA70; RP-A p70; RF-A protein 1
• UniProt Entry Name: RFA1_HUMAN

Uniprot Description

RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: nucleoplasm; male germ cell nucleus; lateral element; PML body; DNA replication factor A complex; nucleus

Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding

Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; base-excision repair; homeostasis of number of cells within a tissue; nucleotide-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; positive regulation of cell proliferation; nucleotide-excision repair, DNA damage removal; hemopoiesis; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA-dependent DNA replication; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle