$380/7 mL (RTU) | $360/0.1 mL (Concentrate) | $585/0.5 mL (Concentrate) | $890/1 mL (Concentrate) | $470/0.2 mL (Concentrate) | $550/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,745/5x1 mL (Concentrate)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using C3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat liver using C3 antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using C3 antibody at dilution of 1:200 (40x lens).)
Western Blot (WB) (Western Blot:Sample: Recombinant PRM1, Human.)
Western Blot (WB) (Western blot analysis of extracts ofvarious cell lines, using GPX4 antibody (MBS126523) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human esophageal cancer using GPX4 Antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse spleen using GPX4 Antibody at dilution of 1:200 (40x lens).)
Testing Data (Immunoperoxidase of polyclonal antibody to CTSDS (1:200) on paraformaldehyde-fixed paraffin embedded mouse lung)
Testing Data (Immunogold lableing using melanocytes and anti-CTSDS Ab)
Immunofluorescence (IF) (Immunofluorescence- anti CTSD at 1/100 dilution in NHI/3T3; cells were fixed with methanol and permeabilized with 0.1% saponin)
Immunofluorescence (IF) (Immunofluorescence - anti-CTSD Ab at 1/100 dilution in RAW264.7 cells; cells were fixed with PFA and permeabilized with 0.05% saponin)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GAPDH antibody (MBS125002) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (MBS128212) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Immunofluorescence (IF) (Immunofluorescence analysis of NIH-3T3 cells using GAPDH Monoclonal Antibody (MBS125002) at dilution of 1:6 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (Immunofluorescence analysis of U-2 OS cells using GAPDH Monoclonal Antibody (MBS125002) at dilution of 1:6 (40x lens). Blue: DAPI for nuclear staining. )
Immunofluorescence (IF) (Immunofluorescence analysis of C6 cells using GAPDH Monoclonal Antibody (MBS125002) at dilution of 1:6 (40x lens). Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western blotAll lanes: Izumo sperm-egg fusion protein 4 antibody at 2ug/ml+rat gonad tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 27,25,18,19 kDaObserved band size: 27 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS1495486 at dilution 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG2 cells using MBS1495486 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human kidney using MBS7001878 at dilution of 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: PC3 whole cell lysate,Hela whole cell lysate,Rat brain tissue,Mouse heart tissue,Mouse liver tissue,Mouse kidney tissue,Mouse brain tissueAll lanes: GPI antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64 KDaObserved band size: 64 KDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7001878 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS7001878 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of PC3 cells using MBS7001878 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunoprecipitation (IP) (Immunoprecipitating GPI in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of MBS7001878 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7001878 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
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