Human calreticulin ELISA Kit | CALR elisa kit

Human Calreticulin, CRT ELISA Kit

Cat. Num. AAA703504

• Gene Names: CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
• Reactivity: Human
• Synonyms: calreticulin; Human Calreticulin; CRT ELISA Kit; CRT; FLJ26680; RO; SSA; cC1qR; Sicca syndrome antigen A (autoantigen Ro; calreticulin) ; autoantigen Ro; CALR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CRT. No significant cross-reactivity or interference between human CRT and analogues was observed.
• Sequence Length: 417

• Samples: Serum, plasma, tissue homogenates, urine
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CALR elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4757900
• NCBI GeneID: 811
• NCBI Accession #: NP_004334.1
• NCBI GenBank Nucleotide #: NM_004343.3
• UniProt Accession #: P27797; Q6IAT4; Q9UDG2
• Molecular Weight: 48,142 Da
• NCBI Official Full Name: calreticulin
• NCBI Official Synonym Full Names: calreticulin
• NCBI Official Symbol: CALR
• NCBI Official Synonym Symbols: RO; CRT; SSA; cC1qR; HEL-S-99n
• NCBI Protein Information: calreticulin; CRP55; ERp60; HACBP; grp60; calregulin; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
• UniProt Protein Name: Calreticulin
• Protein Family: Calreticulin
• UniProt Gene Name: CALR
• UniProt Synonym Gene Names: CRTC; ERp60
• UniProt Entry Name: CALR_HUMAN

NCBI Description

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis 2 PublicationsManual assertion based on experiment in:Ref.20