Human sperm associated antigen 5 ELISA Kit | SPAG5 elisa kit

Human Sperm-associated antigen 5, SPAG5 ELISA Kit

Cat. Num. AAA9342448

• Gene Names: SPAG5; MAP126; DEEPEST; hMAP126
• Reactivity: Human
• Synonyms: sperm associated antigen 5; Human Sperm-associated antigen 5; SPAG5 ELISA Kit; Human Sperm-associated antigen 5 (SPAG5) ELISA kit; DEEPEST; MAP126; hMAP126; astrin; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; SPAG5 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human SPAG5 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SPAG5 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPAG5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPAG5 and SPAG5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPAG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SPAG5 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SPAG5 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPAG5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPAG5 and SPAG5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPAG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 73623035
• NCBI GeneID: 10615
• NCBI Accession #: NP_006452.3
• NCBI GenBank Nucleotide #: NM_006461.3
• UniProt Accession #: Q96R06; O95213; Q9BWE8; Q9NT17; Q9UFE6
• Molecular Weight: 134,422 Da
• NCBI Official Full Name: sperm-associated antigen 5
• NCBI Official Synonym Full Names: sperm associated antigen 5
• NCBI Official Symbol: SPAG5
• NCBI Official Synonym Symbols: MAP126; DEEPEST; hMAP126
• NCBI Protein Information: sperm-associated antigen 5; astrin; mitotic spindle associated protein p126; mitotic spindle-associated protein p126; mitotic spindle coiled-coil related protein
• UniProt Protein Name: Sperm-associated antigen 5
• Protein Family: Sperm-associated antigen
• UniProt Gene Name: SPAG5
• UniProt Synonym Gene Names: MAP126
• UniProt Entry Name: SPAG5_HUMAN

NCBI Description

This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008]

Uniprot Description

SPAG5: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: kinetochore; nucleoplasm; spindle pole; cytoplasm; microtubule organizing center; midbody; nucleus

Molecular Function: protein binding

Biological Process: regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; cell division; spindle organization and biogenesis; chromosome segregation