$225/7 mL (RTU) | $190/0.1 mL (Concentrate) | $285/0.5 mL (Concentrate) | $355/1 mL (Concentrate) | $355/15 mL (RTU) | $200/3 mL (RTU) | $1,600/5x1 mL (Concentrate)
Western Blot (WB) (Cell lysates of NIH-3T3, HepG2, Jurkat, HeLa and SH-SY5Y (each 40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. )
Immunofluorescence (IF) (ICC/IF analysis of CRP in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CRP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Testing Data (Cathepsin B-positiveTHP-1 cells fluorescered after staining withMR-(RR)2. Redfluorophoreconcentrates insidelysosomes (Dr. Brian W.Lee, ICT).)
Testing Data (Healthy cells fluoresce red,dying cells fluoresce green.Jurkat cells were stained withICT's MitoPT JC-1 dye andanalyzed with a fluorescencemicroscope containing a longband path filter (excitation at 490nm and emission >510 nm). Innon-apoptotic healthy cells (2cells at left), the reagent aggregates inside intact mitochondria and fluorescesred. As the mitochondrial membrane potential drops and cells enter apoptosis,the dye disperses throughout the cell. The reagent then assumes a monomericform and fluoresces green (3 cells on right).)
Testing Data #2 (Using a flow cytometer to analyze cells labeled with MitoPT JC-1, theinstrument will measure apoptosis by monitoring the amount of redfluorescence in each region. Healthy cells with intact mitochondria fluorescered due to aggregated MitoPT JC-1 and appear in R2. As the mitochondrialmembrane permeability collapses and cells enter apoptosis, MitoPT JC-1reagent is dispersed throughout the cell, converting to a green fluorescentmonomeric form. The amount of red fluorescence drops as these cells enterR3. In this example, Jurkat cells were either treated with DMSO (negative,non-induced cells) or with staurosporine (apoptotic, induced cells) for 4 hoursand then labeled with MitoPT JC-1 for 15 minutes.)
Western Blot (WB) (Expression analysis of 14-3-3. Anti-14-3-3 antibody (11-1023) was used at 4 µg/ml on (1) MCF-7 and (2) A431 lysates.)
Western Blot (WB) (CHUK Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CHUK antibody detected the CHUK protein (arrow).)
Testing Data (Cathepsin L-positive Jurkat cellsfluoresce red after staining withMR-(FR)2. Red fluorophoreconcentrates inside lysosomes (Dr.Brian W. Lee, ICT).)
Immunofluorescence (IF)/Immunocytochemistry (ICC) (MBS9610384 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(MBS9610384 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Immunohistochemistry (IHC) (MBS9610384 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody)
Western Blot (WB) (Western blot analysis of extracts from various samples, using Cleaved-Caspase 3 (Asp175), p17 Ab.Lane 1: Rat liver, blocked with antigen-specific peptides.Lane 2: Rat liver.Lane 3: Rat spleen.)
Western Blot (WB) (Western blotAll lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2ug/mlLane 1: Hale whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysateLane 5: Jurkats whole cell lysateLane 6: MDA-MB-231 whole cell lysateLane 7: LO2 whole cell lysateLane 8: U251 whole cell lysateLane 9: MCF-7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB) (Western blotAll lanes: GAPDH antibody at 2ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Mouse kidney tissueLane 5: Rat muscle tissueLane 6: Rat lung tissueLane 7: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB) (Western BlotPositive WB detected in: hepG2 cell,mouse brain,mouse skeletal muscleAll lanes: GAPDH antibody at 2.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB) (Western BlotPositive WB detected in:Hela whole cell lysate,A549 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,K562 whole cell lysateAll lanes: GAPDH antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse liver using MBS969811 at dilution 1:50)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS969811 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS969811 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of MCF-7 cells using MBS969811 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Testing Data (Fig.1: Control Cells were stained or unstained as indicated using the Annexin V-FITC Apoptosis Detection Kit.)
Testing Data (Fig.2: Adherent RAW cells were treated with 0.05 ?g/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Celltase and stained with the Annexin VFITC Apoptosis Detection Kit.)