Hamster Bcl-3 Monoclonal Antibody | anti-BCL3 antibody

Bcl-3 Monoclonal Antibody

Cat. Num. AAA190067

• Gene Names: BCL3; BCL4; D19S37
• Applications: ELISA, Immunoblot, Western Blot
• Synonyms: Bcl-3; Monoclonal Antibody; Bcl-3 Monoclonal Antibody; anti-BCL3 antibody

• Host: Hamster
• Clonality: Monoclonal
• Specificity: Bcl-3 [Human and mouse Bcl-3]; This antibody recognizes human and mouse Bcl-3.
• Form/Format: 100 ug IgG in PBS, pH 7.4, purified by Protein G affinity chromatography.

• Applicable Applications for anti-BCL3 antibody: ELISA, Immunoblot
• Application Notes: Immunoblotting: use at 2 ug/ml. A band of approx. 46-52 kDa is detected.; ELISA: end-user should determine optimal concentration for each sample.; Positive control: Jurkat T cell lysate.
• Immunogen: Recombinant mouse Bcl-3-6His.
• Stabilizer: None
• Preservatives: None. Available on request
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-BCL3 antibody

The gene encoding Bcl-3 was first identified as the gene altered in some cases of chronic B cell lymphocytic leukemia and was later found to belong to the I kappa B subfamily of inhibitors of NF kappa B transcription factors. Bcl-3 does not have DNA-binding activity, but it interacts with NF kappa B1 and NF kappa B2 which, when bound to DNA, repress gene transcription. Therefore, Bcl-3 may promote gene expression that is repressed by members of the NF kappa B family. Bcl-3 RNA is highly expressed in spleen and liver with lower levels of expression in heart, skeletal muscle, lung, kidney, and testes.

Product Categories/Family for anti-BCL3 antibody

Apoptosis Antibodies

References

Mitchell, TC et al. (2001) Nature Immunology 2: 397-402.
Watanabe, N et al. (1997) EMBO J 16: 3609-20.
Nolan, GP et al. (1993) Molecular and Cellular Biology 13: 3557-66.

NCBI and Uniprot Product Information

• NCBI GI #: 164664508
• NCBI GeneID: 602
• NCBI Accession #: NP_005169.2
• NCBI GenBank Nucleotide #: NM_005178.4
• UniProt Accession #: P20749
• Molecular Weight: 47,584 Da
• NCBI Official Full Name: B-cell lymphoma 3 protein
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 3
• NCBI Official Symbol: BCL3
• NCBI Official Synonym Symbols: BCL4; D19S37
• NCBI Protein Information: B-cell lymphoma 3 protein; BCL-3; OTTHUMP00000200151; OTTHUMP00000200152; proto-oncogene BCL3; B-cell leukemia/lymphoma 3; B-cell lymphoma 3-encoded protein; chronic lymphatic leukemia protein
• UniProt Protein Name: B-cell lymphoma 3 protein
• Protein Family: B-cell lymphoma 3 protein
• UniProt Gene Name: BCL3
• UniProt Synonym Gene Names: BCL4; D19S37
• UniProt Entry Name: BCL3_HUMAN

NCBI Description

This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. [provided by RefSeq]

Uniprot Description

Function: Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation

By similarity. Ref.4

Subunit structure: Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR. Interacts with CYLD

By similarity. Ref.6 Ref.7

Subcellular location: Nucleus. Cytoplasm

By similarity. Cytoplasm › perinuclear region

By similarity. Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation

Post-translational modification: Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation

By similarity.Activated by phosphorylation.

Involvement in disease: Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.

Sequence similarities: Contains 7 ANK repeats.

Caution: It is uncertain whether Met-1 or Met-9 is the initiator.

Sequence caution: The sequence AAA51815.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA51816.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH64993.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.