BAFF active protein

Recombinant Human BAFF (BLyS)

Cat. Num. AAA142184

• Gene Names: TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: BAFF; Recombinant Human BAFF (BLyS); BAFF Human; B-cell Activating Factor Human Recombinant; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20; TNFSF13B; B-cell Activating Factor; BAFF active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKENKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRCIQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
• Sequence Length: 266

• Solubility: It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The activity is determined by a mouse splenocyte survival assay. The ED50 for this effect is 0.5-2.0 ug/ml.
• Preparation and Storage: Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution BAFF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Related Product Information for BAFF active protein

Description: BAFF Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17007 Dalton. The BAFF is purified by proprietary chromatographic techniques.

Introduction: BAFF binds to tnfrsf13b/taci and tnfrsf17/bcma. Tnfsf13/april binds to the same 2 receptors, together, they form a 2 ligands -2 receptors pathway involved in the stimulation of b- and t-cell function and the regulation of humoral immunity. A third b-cell specific baff-receptor (baffr/br3) promotes the survival of mature b-cells and the b-cell response.B Lymphocyte Stimulator functions as a potent B-cell growth factor in costimulation assays. Administration of BAFF Human recombinant to mice disrupts splenic B-cell and T-cell zones and results in elevated levels of serum immunoglobulin.

Product Categories/Family for BAFF active protein

Cytokines and Growth Factors; Cytokines; B-Cell Activating Factor

NCBI and Uniprot Product Information

• NCBI GI #: 224548983
• NCBI GeneID: 10673
• NCBI Accession #: NP_001139117.1
• NCBI GenBank Nucleotide #: NM_001145645.2
• UniProt Accession #: Q9Y275; Q6FHD6; Q7Z5J2; E0ADT7
• Molecular Weight: 17,578 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13B isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13b
• NCBI Official Symbol: TNFSF13B
• NCBI Official Synonym Symbols: DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13B; ApoL related ligand TALL-1; B-cell-activating factor; B-lymphocyte stimulator; Delta4 BAFF; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; delta BAFF; dendritic cell-derived TNF-like molecule; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor-like protein ZTNF4
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13B
• UniProt Gene Name: TNFSF13B
• UniProt Synonym Gene Names: BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
• UniProt Entry Name: TN13B_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

BAFF: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral; Cell cycle regulation; Cytokine

Chromosomal Location of Human Ortholog: 13q32-q34

Cellular Component: extracellular space; perinuclear region of cytoplasm; cytoplasm; plasma membrane; integral to membrane

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; B cell costimulation; B cell homeostasis; immunoglobulin secretion; T cell costimulation; positive regulation of cell proliferation; positive regulation of germinal center formation; positive regulation of B cell proliferation; immune response; positive regulation of T cell proliferation; signal transduction